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The map label for this gene is fliA [H]

Identifier: 218708840

GI number: 218708840

Start: 862731

End: 863465

Strand: Direct

Name: fliA [H]

Synonym: VS_0840

Alternate gene names: 218708840

Gene position: 862731-863465 (Clockwise)

Preceding gene: 218708839

Following gene: 218708841

Centisome position: 26.15

GC content: 44.35

Gene sequence:

>735_bases
GTGAATAAAGCGCTTACTTACGATCAACATGCTAATCACAATAGCCAGCAGGCTTTTTTTGAGAAGTACTCTGTGTTGGT
TAAGCGTATCGCTCATCATTTGTTGGGGCGATTACCGCCTAATGTATTAGTTGATGACTTGATTCAAGCCGGCATGATTG
GCCTGATTGAAGCGCAACAGAACTATGATGGTACCAAAGGCGCAAGCTTTGAGACGTACGCAGGTATTCGAATTCGTGGG
GCAATGTTGGATGACATTCGCCGCGGGGATTGGGTCCCGAGATCGGTTCATAAAAACAATCGAGAAATCAGTAGTGCAAT
CGCGGAATTAGAGGGCACTCTCAATCGCGATCCCAGTGATGCTGAGGTGGCAAAGCACATGGGGCTGAGTTTAGACCAGT
ATCACAGTGCTTTAACTGATATTAATTGCTCAAAATTGGTAGGGATAGAAGACTTAGGTGTCTCTGATGATGTAATATCT
CCGAATGAAGATTCTCAAGATAATACGCCTTTTCAAGGGGTTGCAGATGAATCATTCCGCCAAGCTTTGATCGACTCGAT
AAAACAACTTCCGGAAAGGGAAGGCCTCGTGCTTTCGCTTTATTACGACGAAGAACTCAATTTAAAAGAGATTGGGGAAG
TATTAGGTGTCAGCGAATCTCGTGTCAGCCAAATACTAAGCCAATCTATGCAGCGTTTACGCACTAAGTTAAGTGCTTGG
ACACAGAACGACTAA

Upstream 100 bases:

>100_bases
AAGGCATTGACCTGGCCAATACCAAAAACACCAAGCGGACACTTGGAGTTTTTTGTTGAAAGGCTGCTGAACCGTACTGA
ATTTATAGAGGAACCATTTG

Downstream 100 bases:

>100_bases
CAACACTGATTTTATTCAGTGGAGGCTAATTTGAACAAAAACATGAAAATTCTCATTGTTGATGATTTTTCAACGATGCG
CCGAATTGTTAAAAACCTAC

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MNKALTYDQHANHNSQQAFFEKYSVLVKRIAHHLLGRLPPNVLVDDLIQAGMIGLIEAQQNYDGTKGASFETYAGIRIRG
AMLDDIRRGDWVPRSVHKNNREISSAIAELEGTLNRDPSDAEVAKHMGLSLDQYHSALTDINCSKLVGIEDLGVSDDVIS
PNEDSQDNTPFQGVADESFRQALIDSIKQLPEREGLVLSLYYDEELNLKEIGEVLGVSESRVSQILSQSMQRLRTKLSAW
TQND

Sequences:

>Translated_244_residues
MNKALTYDQHANHNSQQAFFEKYSVLVKRIAHHLLGRLPPNVLVDDLIQAGMIGLIEAQQNYDGTKGASFETYAGIRIRG
AMLDDIRRGDWVPRSVHKNNREISSAIAELEGTLNRDPSDAEVAKHMGLSLDQYHSALTDINCSKLVGIEDLGVSDDVIS
PNEDSQDNTPFQGVADESFRQALIDSIKQLPEREGLVLSLYYDEELNLKEIGEVLGVSESRVSQILSQSMQRLRTKLSAW
TQND
>Mature_244_residues
MNKALTYDQHANHNSQQAFFEKYSVLVKRIAHHLLGRLPPNVLVDDLIQAGMIGLIEAQQNYDGTKGASFETYAGIRIRG
AMLDDIRRGDWVPRSVHKNNREISSAIAELEGTLNRDPSDAEVAKHMGLSLDQYHSALTDINCSKLVGIEDLGVSDDVIS
PNEDSQDNTPFQGVADESFRQALIDSIKQLPEREGLVLSLYYDEELNLKEIGEVLGVSESRVSQILSQSMQRLRTKLSAW
TQND

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=226, Percent_Identity=48.2300884955752, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1789448, Length=204, Percent_Identity=28.4313725490196, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27237; Mature: 27237

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKALTYDQHANHNSQQAFFEKYSVLVKRIAHHLLGRLPPNVLVDDLIQAGMIGLIEAQQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
NYDGTKGASFETYAGIRIRGAMLDDIRRGDWVPRSVHKNNREISSAIAELEGTLNRDPSD
CCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCH
AEVAKHMGLSLDQYHSALTDINCSKLVGIEDLGVSDDVISPNEDSQDNTPFQGVADESFR
HHHHHHHCCCHHHHHHHHHCCCHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
QALIDSIKQLPEREGLVLSLYYDEELNLKEIGEVLGVSESRVSQILSQSMQRLRTKLSAW
HHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
TQND
CCCC
>Mature Secondary Structure
MNKALTYDQHANHNSQQAFFEKYSVLVKRIAHHLLGRLPPNVLVDDLIQAGMIGLIEAQQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
NYDGTKGASFETYAGIRIRGAMLDDIRRGDWVPRSVHKNNREISSAIAELEGTLNRDPSD
CCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCH
AEVAKHMGLSLDQYHSALTDINCSKLVGIEDLGVSDDVISPNEDSQDNTPFQGVADESFR
HHHHHHHCCCHHHHHHHHHCCCHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
QALIDSIKQLPEREGLVLSLYYDEELNLKEIGEVLGVSESRVSQILSQSMQRLRTKLSAW
HHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
TQND
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]