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The map label for this gene is tgt [H]

Identifier: 218708621

GI number: 218708621

Start: 623420

End: 624556

Strand: Direct

Name: tgt [H]

Synonym: VS_0599

Alternate gene names: 218708621

Gene position: 623420-624556 (Clockwise)

Preceding gene: 218708620

Following gene: 218708622

Centisome position: 18.9

GC content: 44.77

Gene sequence:

>1137_bases
GTGAAATTAAAATACGAACTTAAAAAAACTAATAGCGGCGCACGTCGTGGTCAACTTCAGTTTGAACGCGGTACCGTTGA
AACCCCAGCATTCATGCCTGTAGGTACTTACGGTACGGTTAAAGGCATGACACCTGAAGAAGTAAAAGACACAGGTGCTG
AAATCCTATTAGGTAATACATTCCACCTATGGCTACGTCCTGGTCAAGAAATCATGAAACTGCACGGTGATTTGCACGAT
TTCATGAACTGGAAAGGTCCTATCTTGACTGATTCAGGTGGCTTCCAAGTATTCAGCCTAGGCGCAACGCGTAAAATCAC
TGAAGAAGGTGTTCATTTCCGTAACCCTGTAAACGGTGACAAGATCTTCATGGACGCTGAGAAGTCGATGGAAATCCAAA
AGGATCTAGGCTCAGACATCGTAATGATCTTTGACGAATGTACGCCTTACCCTGCGACGCATAAAGAAGCTAAAGACTCG
ATGGAGATGTCTCTTCGTTGGGCTCAGCGTTCACGCGATCACTTTGATAAGCAAGAAAACCCGAACTCATTGTTCGGTAT
TGTACAAGGTGGCGTATACGAAGACCTTCGTGATGTATCCGTTAAAGGCCTAACAGAAATTGGCTTTGACGGTTATGCAG
TAGGTGGTCTAGCAGTAGGTGAACCAAAAGAAGATATGCACCGCATTCTTGAGCACACATGTCCTCAACTGCCTGAAGAT
AAGCCTCGTTACCTAATGGGTGTAGGCAAACCTGAAGACTTGGTTGAAGGTGTTCGTCGCGGTATTGATATGTTTGACTG
TGTTATGCCAACGCGAAATGCACGTAATGGCCACCTGTTTGTAACGGAAGGTGTGATCAAGATCCGTAATGCGAAGCATA
AAACCGATACAACACCACTAGATTCAGATTGTGACTGTTACACTTGTAAGAACTACAGCAAGTCGTACTTACACCATTTG
GATCGTTGTAACGAAATCCTAGGTGCTCGACTGAACACGATTCACAACCTGCGTTTCTACCAACGAGTAATGTCAGACAT
TCGTCAGTCTATCGATGAAGATCGCTTTGAAGAGTTCGTTGCAGAGTTCTACGCAAGAATGGGGCGTGAAGTGCCACCAC
TAGGTAAAGAATCTTAG

Upstream 100 bases:

>100_bases
TACGCGACCGCTCCTCATAGAACCCACTAGACTGAACTTTTGTTCGGCCTAAGGTTCTGAATTAACAGTCAGATTGTTTC
TCTGGCATATTGGAGGCTTC

Downstream 100 bases:

>100_bases
TATTTCTTTTTTGCGAGCCCTATCTCTCTTCCGAGTGATGGGGCTTGAAAATAAAGGGATACGACCCAATTAGACAAACA
ATTACGAAAATATAATAGAG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKLKYELKKTNSGARRGQLQFERGTVETPAFMPVGTYGTVKGMTPEEVKDTGAEILLGNTFHLWLRPGQEIMKLHGDLHD
FMNWKGPILTDSGGFQVFSLGATRKITEEGVHFRNPVNGDKIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHKEAKDS
MEMSLRWAQRSRDHFDKQENPNSLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRILEHTCPQLPED
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTEGVIKIRNAKHKTDTTPLDSDCDCYTCKNYSKSYLHHL
DRCNEILGARLNTIHNLRFYQRVMSDIRQSIDEDRFEEFVAEFYARMGREVPPLGKES

Sequences:

>Translated_378_residues
MKLKYELKKTNSGARRGQLQFERGTVETPAFMPVGTYGTVKGMTPEEVKDTGAEILLGNTFHLWLRPGQEIMKLHGDLHD
FMNWKGPILTDSGGFQVFSLGATRKITEEGVHFRNPVNGDKIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHKEAKDS
MEMSLRWAQRSRDHFDKQENPNSLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRILEHTCPQLPED
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTEGVIKIRNAKHKTDTTPLDSDCDCYTCKNYSKSYLHHL
DRCNEILGARLNTIHNLRFYQRVMSDIRQSIDEDRFEEFVAEFYARMGREVPPLGKES
>Mature_378_residues
MKLKYELKKTNSGARRGQLQFERGTVETPAFMPVGTYGTVKGMTPEEVKDTGAEILLGNTFHLWLRPGQEIMKLHGDLHD
FMNWKGPILTDSGGFQVFSLGATRKITEEGVHFRNPVNGDKIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHKEAKDS
MEMSLRWAQRSRDHFDKQENPNSLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRILEHTCPQLPED
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTEGVIKIRNAKHKTDTTPLDSDCDCYTCKNYSKSYLHHL
DRCNEILGARLNTIHNLRFYQRVMSDIRQSIDEDRFEEFVAEFYARMGREVPPLGKES

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=367, Percent_Identity=43.0517711171662, Blast_Score=310, Evalue=1e-84,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=80.3763440860215, Blast_Score=646, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=40.8839779005525, Blast_Score=287, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI25146502, Length=249, Percent_Identity=29.718875502008, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920510, Length=361, Percent_Identity=45.4293628808864, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI21355311, Length=256, Percent_Identity=28.90625, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42993; Mature: 42993

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKYELKKTNSGARRGQLQFERGTVETPAFMPVGTYGTVKGMTPEEVKDTGAEILLGNT
CCCEEEECCCCCCCCCCCEEECCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCE
FHLWLRPGQEIMKLHGDLHDFMNWKGPILTDSGGFQVFSLGATRKITEEGVHFRNPVNGD
EEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCHHHHHCCCCCCCCCCCC
KIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHKEAKDSMEMSLRWAQRSRDHFDKQEN
EEEEECHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PNSLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRILEHTCPQLPED
CCCEEHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCC
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTEGVIKIRNAKHKTDTTPL
CCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCC
DSDCDCYTCKNYSKSYLHHLDRCNEILGARLNTIHNLRFYQRVMSDIRQSIDEDRFEEFV
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
AEFYARMGREVPPLGKES
HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLKYELKKTNSGARRGQLQFERGTVETPAFMPVGTYGTVKGMTPEEVKDTGAEILLGNT
CCCEEEECCCCCCCCCCCEEECCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCE
FHLWLRPGQEIMKLHGDLHDFMNWKGPILTDSGGFQVFSLGATRKITEEGVHFRNPVNGD
EEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCHHHHHCCCCCCCCCCCC
KIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHKEAKDSMEMSLRWAQRSRDHFDKQEN
EEEEECHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PNSLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRILEHTCPQLPED
CCCEEHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCC
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTEGVIKIRNAKHKTDTTPL
CCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCC
DSDCDCYTCKNYSKSYLHHLDRCNEILGARLNTIHNLRFYQRVMSDIRQSIDEDRFEEFV
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
AEFYARMGREVPPLGKES
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA