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The map label for this gene is queA

Identifier: 218708620

GI number: 218708620

Start: 622173

End: 623225

Strand: Direct

Name: queA

Synonym: VS_0598

Alternate gene names: 218708620

Gene position: 622173-623225 (Clockwise)

Preceding gene: 218708616

Following gene: 218708621

Centisome position: 18.86

GC content: 45.77

Gene sequence:

>1053_bases
ATGCAAGTTTCAGATTTTCACTTTGACCTACCAGATGAACTCATTGCTCGCTACCCTCAAGAGGAGCGCACAGCAAGCCG
CCTACTTAAATTAGATGGCAACAATGGCAACCTAGCTGATGGTTCGTTTAAAGACGTTTTAGACTTGGTTGAGCCAGGCG
ATCTTGTTGTTTTTAATAACACTCGCGTGATTCCTGCTCGTGTATTCGGTCGCAAGGCATCAGGCGGTAAGCTTGAAGTG
TTGGTTGAACGTATGCTGGATGAGAAAAGCATTCTTGCGCATGTTCGTTGTTCTAAATCACCGAAACCGGGTACCAAATT
GTTCCTTGGTGAGAACGATGAGTATGAAGCTGAAATGGTGGCACGTCATGATGCGTTATTTGAAATCCATTTCACATCCG
ATCAAAGCGTTTTAGAGATTCTTAACAGTGTTGGTCACATGCCACTGCCTCCTTACATCGATCGTCCAGATGAAGACGCC
GATAAAGAGCGCTACCAAACTGTCTATAATGAAAAGCCGGGTGCAGTTGCTGCGCCAACAGCAGGCCTTCACTTTGATGA
CAAGCTAATGGCTGACATGAAAGAAAAAGGTGTTGAGTTTGCTTACGTGACGCTTCACGTTGGTGCTGGTACGTTCCAGC
CAGTAAAAGTCGATAATATCAATGACCACCACATGCATGCTGAGTACGTTGAAGTACCGCAAGAAGTGGTAGATGCCGTT
GCAGCTGCAAAAGCTCGCGGCGGACGCATCATTGCCGTTGGTACAACCTCTGTGCGTTCACTAGAAAGTGCGGCTCAAGA
TGCAGTGAAAAATGGCACTGAGTTAGTTCCTTTCTTTGGTGACACAGAAATCTTCATCTTCCCAGGTTATGAATATCAGC
TGGTGGATTGCTTAATTACCAACTTCCACTTACCAGAATCAACGCTGATTATGTTGGTGAGTGCATTTGCTGGCTACGAC
AATGTGATGGGTGCATACGATCACGCAGTGAAGAGCGAATACCGTTTCTTCAGCTACGGTGATGCGATGTTCATCAATAA
GAAAACGAGCTAA

Upstream 100 bases:

>100_bases
GTTTCTGGTAACGATTTACTTACCGAAACGATTGTTCGGCAACTTCGACTTAATATTCGTCAACGGCATTAATATCATCA
ACTAAGACAAGACTCGACAC

Downstream 100 bases:

>100_bases
TTTTAGCTGGTTATAAACGATATAAGCAGAATTTACGGGTGCTAGCAGTAGGCTAGGAGTCGGGCAGGGTGTCTGTCTAA
TCTCTCGCAAGGAATACGCG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MQVSDFHFDLPDELIARYPQEERTASRLLKLDGNNGNLADGSFKDVLDLVEPGDLVVFNNTRVIPARVFGRKASGGKLEV
LVERMLDEKSILAHVRCSKSPKPGTKLFLGENDEYEAEMVARHDALFEIHFTSDQSVLEILNSVGHMPLPPYIDRPDEDA
DKERYQTVYNEKPGAVAAPTAGLHFDDKLMADMKEKGVEFAYVTLHVGAGTFQPVKVDNINDHHMHAEYVEVPQEVVDAV
AAAKARGGRIIAVGTTSVRSLESAAQDAVKNGTELVPFFGDTEIFIFPGYEYQLVDCLITNFHLPESTLIMLVSAFAGYD
NVMGAYDHAVKSEYRFFSYGDAMFINKKTS

Sequences:

>Translated_350_residues
MQVSDFHFDLPDELIARYPQEERTASRLLKLDGNNGNLADGSFKDVLDLVEPGDLVVFNNTRVIPARVFGRKASGGKLEV
LVERMLDEKSILAHVRCSKSPKPGTKLFLGENDEYEAEMVARHDALFEIHFTSDQSVLEILNSVGHMPLPPYIDRPDEDA
DKERYQTVYNEKPGAVAAPTAGLHFDDKLMADMKEKGVEFAYVTLHVGAGTFQPVKVDNINDHHMHAEYVEVPQEVVDAV
AAAKARGGRIIAVGTTSVRSLESAAQDAVKNGTELVPFFGDTEIFIFPGYEYQLVDCLITNFHLPESTLIMLVSAFAGYD
NVMGAYDHAVKSEYRFFSYGDAMFINKKTS
>Mature_350_residues
MQVSDFHFDLPDELIARYPQEERTASRLLKLDGNNGNLADGSFKDVLDLVEPGDLVVFNNTRVIPARVFGRKASGGKLEV
LVERMLDEKSILAHVRCSKSPKPGTKLFLGENDEYEAEMVARHDALFEIHFTSDQSVLEILNSVGHMPLPPYIDRPDEDA
DKERYQTVYNEKPGAVAAPTAGLHFDDKLMADMKEKGVEFAYVTLHVGAGTFQPVKVDNINDHHMHAEYVEVPQEVVDAV
AAAKARGGRIIAVGTTSVRSLESAAQDAVKNGTELVPFFGDTEIFIFPGYEYQLVDCLITNFHLPESTLIMLVSAFAGYD
NVMGAYDHAVKSEYRFFSYGDAMFINKKTS

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=70.5202312138728, Blast_Score=513, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_VIBSL (B7VJR7)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416241.1
- GeneID:   7160218
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0598
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38872; Mature: 38872

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSDFHFDLPDELIARYPQEERTASRLLKLDGNNGNLADGSFKDVLDLVEPGDLVVFNN
CCCCCCCCCCCHHHHHHCCCHHHHHHHEEEEECCCCCCCCCCHHHHHHHCCCCCEEEECC
TRVIPARVFGRKASGGKLEVLVERMLDEKSILAHVRCSKSPKPGTKLFLGENDEYEAEMV
CEEEEHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHH
ARHDALFEIHFTSDQSVLEILNSVGHMPLPPYIDRPDEDADKERYQTVYNEKPGAVAAPT
HHCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCC
AGLHFDDKLMADMKEKGVEFAYVTLHVGAGTFQPVKVDNINDHHMHAEYVEVPQEVVDAV
CCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHH
AAAKARGGRIIAVGTTSVRSLESAAQDAVKNGTELVPFFGDTEIFIFPGYEYQLVDCLIT
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCEEEEHHHH
NFHLPESTLIMLVSAFAGYDNVMGAYDHAVKSEYRFFSYGDAMFINKKTS
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCEEEEECCCC
>Mature Secondary Structure
MQVSDFHFDLPDELIARYPQEERTASRLLKLDGNNGNLADGSFKDVLDLVEPGDLVVFNN
CCCCCCCCCCCHHHHHHCCCHHHHHHHEEEEECCCCCCCCCCHHHHHHHCCCCCEEEECC
TRVIPARVFGRKASGGKLEVLVERMLDEKSILAHVRCSKSPKPGTKLFLGENDEYEAEMV
CEEEEHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHH
ARHDALFEIHFTSDQSVLEILNSVGHMPLPPYIDRPDEDADKERYQTVYNEKPGAVAAPT
HHCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCC
AGLHFDDKLMADMKEKGVEFAYVTLHVGAGTFQPVKVDNINDHHMHAEYVEVPQEVVDAV
CCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHH
AAAKARGGRIIAVGTTSVRSLESAAQDAVKNGTELVPFFGDTEIFIFPGYEYQLVDCLIT
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCEEEEHHHH
NFHLPESTLIMLVSAFAGYDNVMGAYDHAVKSEYRFFSYGDAMFINKKTS
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA