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The map label for this gene is phoB [H]

Identifier: 218708602

GI number: 218708602

Start: 601122

End: 601811

Strand: Direct

Name: phoB [H]

Synonym: VS_0579

Alternate gene names: 218708602

Gene position: 601122-601811 (Clockwise)

Preceding gene: 218708598

Following gene: 218708603

Centisome position: 18.22

GC content: 44.78

Gene sequence:

>690_bases
ATGTCGAGAAGGATTCTGGTCGTTGAAGATGAAGCACCTATTCGTGAGATGTTGTGTTTTGTACTTGAACAAAAAGGCTA
CCAAGCAGTAGAGGCTGAAGATTACGATACAGCGGTAAACAAGCTATGTGAACCTTTCCCAGATCTAGTATTACTAGATT
GGATGCTACCTGGTGGTAGCGGGATAAACTTTATCAAGCACATGAAGCGTGAAGAGTTAACTCGCAACATCCCAGTGGTG
ATGCTGACGGCTCGTGGTGAAGAAGAAGATAAGGTTCGCGGTTTAGAAGTTGGTGCTGACGACTACATTACCAAACCATT
TTCGCCAAAAGAGTTGGTCGCTCGTCTGAAAGCGGTTATTCGCCGTGTAACACCAACGGCATTGGAAGATGTGATAGATG
TTCAAGGGCTTAAATTAGACCCTGTATCTCACCGCGTTACCGCAAGTGAAGGTCCAGTTGATATGGGCCCAACCGAATTC
AAAATGCTGCATTTCTTTATGACTCACCAAGAGCGAGTATACAGTCGAGAGCAACTGCTCAATAACGTTTGGGGTACCAA
CGTTTACGTTGAAGATCGTACGGTTGATGTTCATATTCGACGTTTACGCAAAGCACTTGAATCTGCTGGTCATGACAAAT
TAATTCAAACGGTTCGCGGCGCAGGCTACCGTTTTTCTACAAAGGCTTAA

Upstream 100 bases:

>100_bases
TTGAGACAGTTATATGAAAATTTGTTTTCAAAGGTCACAAAAGTGTCATAATTAAACCAGATAATGCAATGCGTTGATTA
AATACTAAGGCTATTCAATT

Downstream 100 bases:

>100_bases
CGTGGCTTCACTGCCCAGTTTATGGAGTCCTGAATGGTTGAGAAGTTAACCTGGAAGAAGCTGGCTTGGGAGCTGGCTTT
TTTTTACGCACCTTGGATTA

Product: DNA-binding response regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDTAVNKLCEPFPDLVLLDWMLPGGSGINFIKHMKREELTRNIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTASEGPVDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALESAGHDKLIQTVRGAGYRFSTKA

Sequences:

>Translated_229_residues
MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDTAVNKLCEPFPDLVLLDWMLPGGSGINFIKHMKREELTRNIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTASEGPVDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALESAGHDKLIQTVRGAGYRFSTKA
>Mature_228_residues
SRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDTAVNKLCEPFPDLVLLDWMLPGGSGINFIKHMKREELTRNIPVVM
LTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTASEGPVDMGPTEFK
MLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALESAGHDKLIQTVRGAGYRFSTKA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=81.140350877193, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=38.5964912280702, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=36.6379310344828, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=35.4545454545455, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=35.4260089686099, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=34.6846846846847, Blast_Score=136, Evalue=9e-34,
Organism=Escherichia coli, GI1790860, Length=224, Percent_Identity=36.6071428571429, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1790863, Length=230, Percent_Identity=36.9565217391304, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=32, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=36.6812227074236, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=32.7272727272727, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=32.8888888888889, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=30.8695652173913, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI145693140, Length=238, Percent_Identity=29.8319327731092, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=39.4495412844037, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=29.7520661157025, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26123; Mature: 25992

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDTAVNKLCEPFPDLVLLDWMLPGGS
CCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCC
GINFIKHMKREELTRNIPVVMLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVI
CHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRVTPTALEDVIDVQGLKLDPVSHRVTASEGPVDMGPTEFKMLHFFMTHQERVYSREQLL
HHCCHHHHHHHHHHCCCEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NNVWGTNVYVEDRTVDVHIRRLRKALESAGHDKLIQTVRGAGYRFSTKA
HHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCC
>Mature Secondary Structure 
SRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDTAVNKLCEPFPDLVLLDWMLPGGS
CCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCC
GINFIKHMKREELTRNIPVVMLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVI
CHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRVTPTALEDVIDVQGLKLDPVSHRVTASEGPVDMGPTEFKMLHFFMTHQERVYSREQLL
HHCCHHHHHHHHHHCCCEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NNVWGTNVYVEDRTVDVHIRRLRKALESAGHDKLIQTVRGAGYRFSTKA
HHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]