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The map label for this gene is mepA [H]

Identifier: 218708597

GI number: 218708597

Start: 596646

End: 597485

Strand: Direct

Name: mepA [H]

Synonym: VS_0574

Alternate gene names: 218708597

Gene position: 596646-597485 (Clockwise)

Preceding gene: 218708596

Following gene: 218708598

Centisome position: 18.08

GC content: 41.55

Gene sequence:

>840_bases
ATGAGGTTATTTTGTACATTGGCTCTTATCTTTTGTTCGTTTGTTGTATCTGCAACCTCGTGGGAAAAAGAGAAAAGCCC
AACAAGAAATTCAACCTCATCCATAGGTAGTTACGCTAATGGTTGTCTTGACGGGGCGCAAGCTTTGCCAATTAATGGGC
AAGGTTATCAGGTGATCCGCCCTCAGAGAGAGCGCTATTATGGCCACACAGAGTCAATTCAGTTTATTGAAAGGCTAGGT
TTAACTACAAGCAAACAACTCAACACAAATCTATTGGTGGGTGATATTTCTCTGCCTCGTGGAGGGCGTTTTTCTTCGGG
TCATTCAAGTCACCAGACTGGTTTGGATATTGATATATGGTTAAGGCTCACTGATAAAAAACTTTCAGAGAATGAACTTG
CCGTACCTAAGCCATTGAGCGTTGTCGATCTCACTAACTACAAATTGCGTGAATCGAATTGGACCGATGATCATTTTCAA
GTGATTCGCTATGCAGCCAAAGATGAAAAAGTGGTGCGTATTTTTGTTCACCCAGTTATTAAGCACACCCTTTGTGAACA
AGAAAACTCTGATGCCAATGGGAGAGCTTGGTTAGGTAAAATTAGGCCTTGGTGGGGACATCATTCTCATTTTCATGTTC
GTTTAAAATGCCCTAAAGGCAGCTCAAACTGTATAGCTCAAGCAAGCCCTCCTAAAGGTGATGGTTGTGGAGATGAACTT
GCAAGTTGGGCTCCAAAGACCATTCCTGTTCCTCCACCTAAAAAAGTCGCTAAAAGTAAGAAAAAAAAGAGAGTTAACGT
GATGCCAAGTCAATGTTTGGCCTTGATATCAAATAAGTAA

Upstream 100 bases:

>100_bases
GAGATAACTCTCAAGCTATCCCAAAAATATCACTCTCGACATACACCTATTCTCTAATGACGACTAGTATAAAAACAGTG
ATCGTTTATTTGGAATTGTT

Downstream 100 bases:

>100_bases
TCCTGTTCATCGAGAATTTTTGCAGTGGTAATGCCACTGGTTTTCTCGACTCTAAAACTGGAGAAATATCACACTTTTGT
CTAGAGTTTATAAACACGAG

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MRLFCTLALIFCSFVVSATSWEKEKSPTRNSTSSIGSYANGCLDGAQALPINGQGYQVIRPQRERYYGHTESIQFIERLG
LTTSKQLNTNLLVGDISLPRGGRFSSGHSSHQTGLDIDIWLRLTDKKLSENELAVPKPLSVVDLTNYKLRESNWTDDHFQ
VIRYAAKDEKVVRIFVHPVIKHTLCEQENSDANGRAWLGKIRPWWGHHSHFHVRLKCPKGSSNCIAQASPPKGDGCGDEL
ASWAPKTIPVPPPKKVAKSKKKKRVNVMPSQCLALISNK

Sequences:

>Translated_279_residues
MRLFCTLALIFCSFVVSATSWEKEKSPTRNSTSSIGSYANGCLDGAQALPINGQGYQVIRPQRERYYGHTESIQFIERLG
LTTSKQLNTNLLVGDISLPRGGRFSSGHSSHQTGLDIDIWLRLTDKKLSENELAVPKPLSVVDLTNYKLRESNWTDDHFQ
VIRYAAKDEKVVRIFVHPVIKHTLCEQENSDANGRAWLGKIRPWWGHHSHFHVRLKCPKGSSNCIAQASPPKGDGCGDEL
ASWAPKTIPVPPPKKVAKSKKKKRVNVMPSQCLALISNK
>Mature_279_residues
MRLFCTLALIFCSFVVSATSWEKEKSPTRNSTSSIGSYANGCLDGAQALPINGQGYQVIRPQRERYYGHTESIQFIERLG
LTTSKQLNTNLLVGDISLPRGGRFSSGHSSHQTGLDIDIWLRLTDKKLSENELAVPKPLSVVDLTNYKLRESNWTDDHFQ
VIRYAAKDEKVVRIFVHPVIKHTLCEQENSDANGRAWLGKIRPWWGHHSHFHVRLKCPKGSSNCIAQASPPKGDGCGDEL
ASWAPKTIPVPPPKKVAKSKKKKRVNVMPSQCLALISNK

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=260, Percent_Identity=42.3076923076923, Blast_Score=223, Evalue=9e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 31171; Mature: 31171

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFCTLALIFCSFVVSATSWEKEKSPTRNSTSSIGSYANGCLDGAQALPINGQGYQVIR
CHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCEEEEC
PQRERYYGHTESIQFIERLGLTTSKQLNTNLLVGDISLPRGGRFSSGHSSHQTGLDIDIW
CHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEE
LRLTDKKLSENELAVPKPLSVVDLTNYKLRESNWTDDHFQVIRYAAKDEKVVRIFVHPVI
EEEECCCCCCCCCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCEEEEEHHHHHH
KHTLCEQENSDANGRAWLGKIRPWWGHHSHFHVRLKCPKGSSNCIAQASPPKGDGCGDEL
HHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCHHHH
ASWAPKTIPVPPPKKVAKSKKKKRVNVMPSQCLALISNK
HHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MRLFCTLALIFCSFVVSATSWEKEKSPTRNSTSSIGSYANGCLDGAQALPINGQGYQVIR
CHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCEEEEC
PQRERYYGHTESIQFIERLGLTTSKQLNTNLLVGDISLPRGGRFSSGHSSHQTGLDIDIW
CHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEE
LRLTDKKLSENELAVPKPLSVVDLTNYKLRESNWTDDHFQVIRYAAKDEKVVRIFVHPVI
EEEECCCCCCCCCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHCCCCEEEEEHHHHHH
KHTLCEQENSDANGRAWLGKIRPWWGHHSHFHVRLKCPKGSSNCIAQASPPKGDGCGDEL
HHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCHHHH
ASWAPKTIPVPPPKKVAKSKKKKRVNVMPSQCLALISNK
HHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA