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The map label for this gene is clpB [H]

Identifier: 218708596

GI number: 218708596

Start: 587805

End: 590378

Strand: Direct

Name: clpB [H]

Synonym: VS_0562

Alternate gene names: 218708596

Gene position: 587805-590378 (Clockwise)

Preceding gene: 218708595

Following gene: 218708597

Centisome position: 17.82

GC content: 43.75

Gene sequence:

>2574_bases
ATGCGTCTCGATCGATTCACTAGTAAATTCCAAATTGCGATATCTGATGCTCAGTCGCTCGCATTAGGTCGTGATCACCA
ATATATAGAACCTGTACACCTAATGGTGTCTTTGCTTAATCAAGATGGCAGTGCGATTCGTCCATTGCTCACTATGTTGA
ATATTGATGTTGTTCAATTGCGTTCTAAATTGAGCGAAATATTAGACCGAGTCCCAAAAGTAAGCGGTATCGGTGGTGAT
GTTCAGCTCTCTAACGCAATGGGAACGCTATTCAACCTATGTGACAAGGTCGCGCAAAAGCGTCAAGACAGTTACATATC
TTCAGAAGTATTCCTACTCGCTGCAATAGAAGACAAAGGACCTCTAGGTAACTTACTTAAAGAGCTAGGCCTTACTGAGC
AAAAATTATCTCAAGCTATCGAGCAAGTTCGTGGTGGTCAAAAGGTTGATGACCCGAATGCGGAAGATAGACGCCAAGCT
TTAGAGAAGTTCACCATTGATCTGACCGAGCGAGCAGAGCAAGGCAAGCTAGATCCTGTGATTGGTCGTGATGATGAAAT
TCGTCGCACGATTCAAGTCCTTCAACGTCGTACTAAGAACAACCCTGTCATTATCGGTCAACCCGGTGTGGGTAAAACTG
CGATTGTCGAAGGTTTGGCTCAGCGTATTATCAATAATGAAGTGCCTGAAGGCTTAAGAGGCCGACGAGTGCTTTCATTA
GACATGGGCTCATTAGTTGCGGGTGCTAAATATCGTGGTGAATTCGAAGAGCGTCTAAAATCAGTGCTTAATGAATTATC
GAAAGAAGAAGGCAATGTCATTCTCTTTATCGATGAAATTCATACTATGGTCGGCGCAGGTAAAGGCGAAGGCTCTATGG
ATGCGGGTAACATGCTGAAACCAGCATTAGCTCGTGGTGAACTGCATTGTGTCGGCGCAACAACGCTCGATGAATACCGA
CAATATATAGAGAAAGACCCGGCTTTAGAGCGTAGATTCCAAAAAGTAATAGTGGATGAACCCACCGTTGAAGACACGGT
AGCGATTCTTCGTGGTTTGAAAGAACGTTATGAGCTTCACCATCATGTAGAAATTACCGATCCTGCTATCGTAGCGGCGG
CAACGCTATCTCACCGATACGTTTCAGATCGTCAGTTACCAGATAAGGCGATTGATCTCATTGATGAAGCGGCTTCGAGT
ATTCGAATGCAGATAGATTCAAAGCCCGAGTCATTGGATAAGTTAGAGCGCAAAATCATTCAGTTGAAGATCGAACAGCA
AGCTCTGACGAATGAAAATGATGAGGCCAGTGAAAAACGCCTGCGCACGCTACAAAGCGAACTTTCTGAGAAAGAACGAG
ATTTCGCAGAACTTGAAGAAGTTTGGAACGCTGAAAAAGCGGCGCTGTCTGGTACGCAACATATTAAATCAGAGCTAGAA
CAAGCTCGAATGGATATGGATTTTGCACGACGCGCTGGTGACCTTAACCGTATGTCTGAATTGCAATATGGTCGAATCCC
AGAATTGGAGAAGCAGTTAGATCTCGCGACTCAAGCTGAAATGCAAGAAATGACCTTATTACGTAATAAGGTGACCGACG
CTGAGATCGCCGATGTCCTTTCAAAGCAAACCGGTATTCCTGTTTCTAAAATGCTAGAAGCAGAAAAGGAGAAGCTTCTG
AAAATGGAAGGTGTTCTGCATAAGCGGGTTATCGGTCAAGGGGAAGCGGTCGAAGTGGTATCGAATGCGATTCGTCGTAG
CCGTGCTGGTTTGTCTGATCCGAATAAACCGATTGGTTCGTTCTTGTTCTTAGGGCCAACAGGTGTCGGTAAAACGGAAC
TGTGTAAAACATTAGCTAACTTTATGTTCGATAGTGAAGATGCGATGGTTCGTATTGATATGTCTGAGTTCATGGAAAAA
CACTCTGTTGCTCGTTTAGTTGGTGCTCCCCCTGGTTATGTGGGTTATGAAGAAGGTGGGTACTTAACCGAAGCTGTTCG
TCGAAAACCTTATTCTGTCATTCTGCTTGATGAGGTAGAGAAAGCTCACCCAGATGTTTTCAATATTCTATTGCAGGTTT
TGGATGATGGACGATTAACTGACGGACAAGGTCGCACCGTCGATTTTAGAAATACCGTGGTAATCATGACATCGAACCTA
GGTTCAACTCGTATTCAAGAGAACTTCAATACATTGGATTACCAAGGCATTAAAAATGAGGTTATGGAAGTTGTGGGTAA
ACATTTCCGACCTGAGTTTTTGAATCGAGTTGATGAAAGTGTCGTGTTCCATCCATTAGGCCAAGAGCACATCGAGTCAA
TTGCCGCTATTCAGCTTGAGCATTTGAAAATGCGAATGGAAGACAATGGTTATGAACTCGAAGTTTCAGAAAAAGCGCTC
AAGCTAATCTCTCAAGTTGGTTTTGATCCTGTATATGGTGCAAGGCCTCTTAAAAGAGCAATTCAGCAGAGTGTTGAGAA
CCCATTAGCGAAAGCGATACTTGCGGGGAAAATCAATCCGGATAAGAAAGTGCAGCTATTAGTGAATAACGACCGAATTA
TTGCTCACCAATAA

Upstream 100 bases:

>100_bases
CATATCATCTCTATTCTTCATCCTTATCCCTTGAAAATCTGCGTTACCGTATCCATCTTCTAACCATAAGATAAACATAT
CTAAGAGTAGGAAGGTTGGC

Downstream 100 bases:

>100_bases
TCAAGGTAAGAACGATAGGTACGATTAGTCGAAATTAAGACGCAATCGTACCTATTTTGAACGCTTTGGGTGAATAATGT
CCGAACGATCGCCTGTGGCG

Product: chaperone ClpB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVSLLNQDGSAIRPLLTMLNIDVVQLRSKLSEILDRVPKVSGIGGD
VQLSNAMGTLFNLCDKVAQKRQDSYISSEVFLLAAIEDKGPLGNLLKELGLTEQKLSQAIEQVRGGQKVDDPNAEDRRQA
LEKFTIDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
DMGSLVAGAKYRGEFEERLKSVLNELSKEEGNVILFIDEIHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVIVDEPTVEDTVAILRGLKERYELHHHVEITDPAIVAAATLSHRYVSDRQLPDKAIDLIDEAASS
IRMQIDSKPESLDKLERKIIQLKIEQQALTNENDEASEKRLRTLQSELSEKERDFAELEEVWNAEKAALSGTQHIKSELE
QARMDMDFARRAGDLNRMSELQYGRIPELEKQLDLATQAEMQEMTLLRNKVTDAEIADVLSKQTGIPVSKMLEAEKEKLL
KMEGVLHKRVIGQGEAVEVVSNAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELCKTLANFMFDSEDAMVRIDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSTRIQENFNTLDYQGIKNEVMEVVGKHFRPEFLNRVDESVVFHPLGQEHIESIAAIQLEHLKMRMEDNGYELEVSEKAL
KLISQVGFDPVYGARPLKRAIQQSVENPLAKAILAGKINPDKKVQLLVNNDRIIAHQ

Sequences:

>Translated_857_residues
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVSLLNQDGSAIRPLLTMLNIDVVQLRSKLSEILDRVPKVSGIGGD
VQLSNAMGTLFNLCDKVAQKRQDSYISSEVFLLAAIEDKGPLGNLLKELGLTEQKLSQAIEQVRGGQKVDDPNAEDRRQA
LEKFTIDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
DMGSLVAGAKYRGEFEERLKSVLNELSKEEGNVILFIDEIHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVIVDEPTVEDTVAILRGLKERYELHHHVEITDPAIVAAATLSHRYVSDRQLPDKAIDLIDEAASS
IRMQIDSKPESLDKLERKIIQLKIEQQALTNENDEASEKRLRTLQSELSEKERDFAELEEVWNAEKAALSGTQHIKSELE
QARMDMDFARRAGDLNRMSELQYGRIPELEKQLDLATQAEMQEMTLLRNKVTDAEIADVLSKQTGIPVSKMLEAEKEKLL
KMEGVLHKRVIGQGEAVEVVSNAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELCKTLANFMFDSEDAMVRIDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSTRIQENFNTLDYQGIKNEVMEVVGKHFRPEFLNRVDESVVFHPLGQEHIESIAAIQLEHLKMRMEDNGYELEVSEKAL
KLISQVGFDPVYGARPLKRAIQQSVENPLAKAILAGKINPDKKVQLLVNNDRIIAHQ
>Mature_857_residues
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVSLLNQDGSAIRPLLTMLNIDVVQLRSKLSEILDRVPKVSGIGGD
VQLSNAMGTLFNLCDKVAQKRQDSYISSEVFLLAAIEDKGPLGNLLKELGLTEQKLSQAIEQVRGGQKVDDPNAEDRRQA
LEKFTIDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
DMGSLVAGAKYRGEFEERLKSVLNELSKEEGNVILFIDEIHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDPALERRFQKVIVDEPTVEDTVAILRGLKERYELHHHVEITDPAIVAAATLSHRYVSDRQLPDKAIDLIDEAASS
IRMQIDSKPESLDKLERKIIQLKIEQQALTNENDEASEKRLRTLQSELSEKERDFAELEEVWNAEKAALSGTQHIKSELE
QARMDMDFARRAGDLNRMSELQYGRIPELEKQLDLATQAEMQEMTLLRNKVTDAEIADVLSKQTGIPVSKMLEAEKEKLL
KMEGVLHKRVIGQGEAVEVVSNAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELCKTLANFMFDSEDAMVRIDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSTRIQENFNTLDYQGIKNEVMEVVGKHFRPEFLNRVDESVVFHPLGQEHIESIAAIQLEHLKMRMEDNGYELEVSEKAL
KLISQVGFDPVYGARPLKRAIQQSVENPLAKAILAGKINPDKKVQLLVNNDRIIAHQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=302, Percent_Identity=37.4172185430464, Blast_Score=203, Evalue=7e-52,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=75.7292882147024, Blast_Score=1348, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=398, Percent_Identity=39.1959798994975, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6320464, Length=709, Percent_Identity=50.3526093088858, Blast_Score=744, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=867, Percent_Identity=41.7531718569781, Blast_Score=665, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95926; Mature: 95926

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVSLLNQDGSAIRPLLTMLNIDVVQL
CCCHHHCCHHEEEECCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RSKLSEILDRVPKVSGIGGDVQLSNAMGTLFNLCDKVAQKRQDSYISSEVFLLAAIEDKG
HHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PLGNLLKELGLTEQKLSQAIEQVRGGQKVDDPNAEDRRQALEKFTIDLTERAEQGKLDPV
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGSLVAGAKYRGEFEERLKSVLNELSKEEGNVILFIDEIHTMVGAGKGEGSMDAGNMLK
CHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRQYIEKDPALERRFQKVIVDEPTVEDTVAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAATLSHRYVSDRQLPDKAIDLIDEAASSIRMQIDSKPESLDKLERKII
HCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEHCCCCCHHHHHHHHHHH
QLKIEQQALTNENDEASEKRLRTLQSELSEKERDFAELEEVWNAEKAALSGTQHIKSELE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHH
QARMDMDFARRAGDLNRMSELQYGRIPELEKQLDLATQAEMQEMTLLRNKVTDAEIADVL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SKQTGIPVSKMLEAEKEKLLKMEGVLHKRVIGQGEAVEVVSNAIRRSRAGLSDPNKPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKTLANFMFDSEDAMVRIDMSEFMEKHSVARLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSTRIQENFNTLDYQGIKNEVMEVVGKHFRPEFLNRVDESVVFHPLGQEHIESIAAIQLE
CCHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
HLKMRMEDNGYELEVSEKALKLISQVGFDPVYGARPLKRAIQQSVENPLAKAILAGKINP
HHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DKKVQLLVNNDRIIAHQ
CCEEEEEEECCEEEECC
>Mature Secondary Structure
MRLDRFTSKFQIAISDAQSLALGRDHQYIEPVHLMVSLLNQDGSAIRPLLTMLNIDVVQL
CCCHHHCCHHEEEECCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RSKLSEILDRVPKVSGIGGDVQLSNAMGTLFNLCDKVAQKRQDSYISSEVFLLAAIEDKG
HHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PLGNLLKELGLTEQKLSQAIEQVRGGQKVDDPNAEDRRQALEKFTIDLTERAEQGKLDPV
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINNEVPEGLRGRRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGSLVAGAKYRGEFEERLKSVLNELSKEEGNVILFIDEIHTMVGAGKGEGSMDAGNMLK
CHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRQYIEKDPALERRFQKVIVDEPTVEDTVAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAATLSHRYVSDRQLPDKAIDLIDEAASSIRMQIDSKPESLDKLERKII
HCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEHCCCCCHHHHHHHHHHH
QLKIEQQALTNENDEASEKRLRTLQSELSEKERDFAELEEVWNAEKAALSGTQHIKSELE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHH
QARMDMDFARRAGDLNRMSELQYGRIPELEKQLDLATQAEMQEMTLLRNKVTDAEIADVL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SKQTGIPVSKMLEAEKEKLLKMEGVLHKRVIGQGEAVEVVSNAIRRSRAGLSDPNKPIGS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKTLANFMFDSEDAMVRIDMSEFMEKHSVARLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSTRIQENFNTLDYQGIKNEVMEVVGKHFRPEFLNRVDESVVFHPLGQEHIESIAAIQLE
CCHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
HLKMRMEDNGYELEVSEKALKLISQVGFDPVYGARPLKRAIQQSVENPLAKAILAGKINP
HHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DKKVQLLVNNDRIIAHQ
CCEEEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]