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The map label for this gene is yfhA [H]

Identifier: 218708552

GI number: 218708552

Start: 537614

End: 538948

Strand: Direct

Name: yfhA [H]

Synonym: VS_0518

Alternate gene names: 218708552

Gene position: 537614-538948 (Clockwise)

Preceding gene: 218708551

Following gene: 218708553

Centisome position: 16.29

GC content: 43.9

Gene sequence:

>1335_bases
ATGGGAACTCAATTTAAGATGGATTCCTTACCAGGTTCTCTTATCGTCGTTGGTGGTGCGTACGAACCCTGGTTATCAGT
GTTAGAACAAGTGGGTTGGCAATGTACCCAGTGTGCAGATTTACGAAAAGCCGATGCCTTAATTACTGATATTGGCCCCT
GCATTGGCATTGTGGATCTTAGTCATGATGAGTTTAGCCTTAATGGTATCGCTAACCTCGTGAGCAACAACAAGCAAGTT
CGATGGCTTGCTTTTATCCGTGAATCGCAATTGAGCTCAGATACAATCTGCCAATTTATCGTTAACTTCTGTATCGACTT
TTTCACGGCACCAATCCCTGATGCTCAGCTATTGAGTACCATTGGTCATCAACTGGGTATGCTCAAACTTGAGCAGAAAG
TATGGCCAAACTACGGTATTAACAACAATATGGGCCTACTCGGTGATTCTGTGGCGGTGAAACGCTTGAGAGACCAAGTC
AAGCGAATTGGCCCCACAGATGTCAGTATTTTGATTTACGGTGAGAGTGGGACAGGAAAAGAGACAATTGCACGTTCCAT
TCATCAAAATTCATCGCGAGCTCAGAAACCATTTTTAACTGTGAATTGCCGTGCTCTATCTGAAATGAGAATAGAGACTG
AGATGTTTGGTATTTCAGCCCCAGCAACGGCAGCGCCTTGTATGTTGGAAGAAGCCGATGGCGGAACCCTCTTACTCAAC
GATGTCCTAGCGATGCCTCGTAATCAACAATTGAACCTTTTACGCTTTTTACAAGAAGGGAAAATTGAAACCACGAAGGG
AGCTAAGTCGGTTGATGTTCGCATTTTGGCTGCTAACTCTTCTGATATTGAAAAGGCATTGATCGAGGGCGACTTCAATG
AAGAGCTTTATCATTACATCAATGTTCTGCGTATTCATGTTCCTAGCTTAAAAGAACGTGTGAGTGATATTTCAGTGCTA
GCGAATCATTTTCTGCGCGAGTACTCAAAAGAGTTTAATGCGCAAGCGAAGAGCTTCTCAGACGATGCTATCCGCTCGAT
GAGTCGCTACCATTGGCCTGGCAACGTTCGTGAATTGATGAATCAGATTAAGCGTGTTGTGTTGATGTCTGATGCTGTCA
TCATTGAGGATCATCAACTGGATTTACCAAAACAGAATGATGAACGCCGCAGTCTGAAAAGTATACGTGAACGTTCAGAG
CGAGATGCTTTACTCATTGTGTTGGAATCTTATGGTGGTCAAGTGTCTTTGGCCGCTAAGGAGCTTGGAGTTTCACGCGC
AACTATGTACCGCTTACTCAATAAACATGGTCTTATATCTGAGGGCGTTGTTTAG

Upstream 100 bases:

>100_bases
TCGGATCTGAATACGATCTTGCGGCAACTTTGTACCCGTATGATTCGTTTAACTCGTGTTAGCCTGATTGAAAAAAATAA
AAACAGAATAAGGAAGAAGG

Downstream 100 bases:

>100_bases
AACGTTCTATTCATTGACTCTATCCGGTGAGTGAAAATTCAAGGGCCACGTATCATACGTGGCTTTTTTGTTTCTGAAGG
ATAGTCCGCTGTTCTATAGA

Product: Sigma-54 dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MGTQFKMDSLPGSLIVVGGAYEPWLSVLEQVGWQCTQCADLRKADALITDIGPCIGIVDLSHDEFSLNGIANLVSNNKQV
RWLAFIRESQLSSDTICQFIVNFCIDFFTAPIPDAQLLSTIGHQLGMLKLEQKVWPNYGINNNMGLLGDSVAVKRLRDQV
KRIGPTDVSILIYGESGTGKETIARSIHQNSSRAQKPFLTVNCRALSEMRIETEMFGISAPATAAPCMLEEADGGTLLLN
DVLAMPRNQQLNLLRFLQEGKIETTKGAKSVDVRILAANSSDIEKALIEGDFNEELYHYINVLRIHVPSLKERVSDISVL
ANHFLREYSKEFNAQAKSFSDDAIRSMSRYHWPGNVRELMNQIKRVVLMSDAVIIEDHQLDLPKQNDERRSLKSIRERSE
RDALLIVLESYGGQVSLAAKELGVSRATMYRLLNKHGLISEGVV

Sequences:

>Translated_444_residues
MGTQFKMDSLPGSLIVVGGAYEPWLSVLEQVGWQCTQCADLRKADALITDIGPCIGIVDLSHDEFSLNGIANLVSNNKQV
RWLAFIRESQLSSDTICQFIVNFCIDFFTAPIPDAQLLSTIGHQLGMLKLEQKVWPNYGINNNMGLLGDSVAVKRLRDQV
KRIGPTDVSILIYGESGTGKETIARSIHQNSSRAQKPFLTVNCRALSEMRIETEMFGISAPATAAPCMLEEADGGTLLLN
DVLAMPRNQQLNLLRFLQEGKIETTKGAKSVDVRILAANSSDIEKALIEGDFNEELYHYINVLRIHVPSLKERVSDISVL
ANHFLREYSKEFNAQAKSFSDDAIRSMSRYHWPGNVRELMNQIKRVVLMSDAVIIEDHQLDLPKQNDERRSLKSIRERSE
RDALLIVLESYGGQVSLAAKELGVSRATMYRLLNKHGLISEGVV
>Mature_443_residues
GTQFKMDSLPGSLIVVGGAYEPWLSVLEQVGWQCTQCADLRKADALITDIGPCIGIVDLSHDEFSLNGIANLVSNNKQVR
WLAFIRESQLSSDTICQFIVNFCIDFFTAPIPDAQLLSTIGHQLGMLKLEQKVWPNYGINNNMGLLGDSVAVKRLRDQVK
RIGPTDVSILIYGESGTGKETIARSIHQNSSRAQKPFLTVNCRALSEMRIETEMFGISAPATAAPCMLEEADGGTLLLND
VLAMPRNQQLNLLRFLQEGKIETTKGAKSVDVRILAANSSDIEKALIEGDFNEELYHYINVLRIHVPSLKERVSDISVLA
NHFLREYSKEFNAQAKSFSDDAIRSMSRYHWPGNVRELMNQIKRVVLMSDAVIIEDHQLDLPKQNDERRSLKSIRERSER
DALLIVLESYGGQVSLAAKELGVSRATMYRLLNKHGLISEGVV

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=386, Percent_Identity=32.3834196891192, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788905, Length=293, Percent_Identity=37.2013651877133, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1788550, Length=428, Percent_Identity=33.6448598130841, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1790299, Length=257, Percent_Identity=36.9649805447471, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI87082117, Length=335, Percent_Identity=32.8358208955224, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=31.7757009345794, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1789087, Length=315, Percent_Identity=32.6984126984127, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1786524, Length=314, Percent_Identity=31.8471337579618, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87081872, Length=316, Percent_Identity=33.5443037974684, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787583, Length=300, Percent_Identity=32, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=30.6962025316456, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87081858, Length=304, Percent_Identity=26.9736842105263, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789828, Length=233, Percent_Identity=25.7510729613734, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49504; Mature: 49373

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTQFKMDSLPGSLIVVGGAYEPWLSVLEQVGWQCTQCADLRKADALITDIGPCIGIVDL
CCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
SHDEFSLNGIANLVSNNKQVRWLAFIRESQLSSDTICQFIVNFCIDFFTAPIPDAQLLST
CCCCCHHHHHHHHHCCCCCEEEHEEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IGHQLGMLKLEQKVWPNYGINNNMGLLGDSVAVKRLRDQVKRIGPTDVSILIYGESGTGK
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
ETIARSIHQNSSRAQKPFLTVNCRALSEMRIETEMFGISAPATAAPCMLEEADGGTLLLN
HHHHHHHHCCCCHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEH
DVLAMPRNQQLNLLRFLQEGKIETTKGAKSVDVRILAANSSDIEKALIEGDFNEELYHYI
HHHCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHH
NVLRIHVPSLKERVSDISVLANHFLREYSKEFNAQAKSFSDDAIRSMSRYHWPGNVRELM
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHCCCCCHHHHHH
NQIKRVVLMSDAVIIEDHQLDLPKQNDERRSLKSIRERSERDALLIVLESYGGQVSLAAK
HHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEHH
ELGVSRATMYRLLNKHGLISEGVV
HHCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GTQFKMDSLPGSLIVVGGAYEPWLSVLEQVGWQCTQCADLRKADALITDIGPCIGIVDL
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
SHDEFSLNGIANLVSNNKQVRWLAFIRESQLSSDTICQFIVNFCIDFFTAPIPDAQLLST
CCCCCHHHHHHHHHCCCCCEEEHEEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IGHQLGMLKLEQKVWPNYGINNNMGLLGDSVAVKRLRDQVKRIGPTDVSILIYGESGTGK
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
ETIARSIHQNSSRAQKPFLTVNCRALSEMRIETEMFGISAPATAAPCMLEEADGGTLLLN
HHHHHHHHCCCCHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEH
DVLAMPRNQQLNLLRFLQEGKIETTKGAKSVDVRILAANSSDIEKALIEGDFNEELYHYI
HHHCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHH
NVLRIHVPSLKERVSDISVLANHFLREYSKEFNAQAKSFSDDAIRSMSRYHWPGNVRELM
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHCCCCCHHHHHH
NQIKRVVLMSDAVIIEDHQLDLPKQNDERRSLKSIRERSERDALLIVLESYGGQVSLAAK
HHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEHH
ELGVSRATMYRLLNKHGLISEGVV
HHCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]