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The map label for this gene is lysS [H]

Identifier: 218708551

GI number: 218708551

Start: 535805

End: 537337

Strand: Direct

Name: lysS [H]

Synonym: VS_0517

Alternate gene names: 218708551

Gene position: 535805-537337 (Clockwise)

Preceding gene: 218708550

Following gene: 218708552

Centisome position: 16.24

GC content: 47.42

Gene sequence:

>1533_bases
ATGACTGATGCTGTTCAAAACGAAAATACACAAGAAGCTTCTTCACCTGAAGAGAACAAACTAATCGCTGAGCGCCGTAG
CAAGCTGGATCATATCCGCCAAAACTGCAAAGCGAACGGTCACCCAAATGACTTCCGTCGTGAGCACCTAGCTGGCGACC
TTCAAGCGGAATTCGGTGAGAAGACTAAGGAAGAGCTAGAAGAGCTTAACCACATCGTTGCGATCGCAGGTCGTATTATG
GCGAAGCGTGGTCCATTTCTAGCGATTCAAGAAACTTCTGGTCGTATCCAAGCGTACGCAGCGAAAGACGTTCAAAAAGT
GCTGAAAGAGAAGTACCAAGGCCTAGATATCGGTGACATCATCGGTGTTAAAGGTGCGCTTCATAAGTCTGGTAAAGGTG
ACCTTTACGTGAACATGGAAGAGTTTGAATTGCTAACGAAAGCACTTCGTCCTCTGCCAGAGAAGTTCCACGGTCTAACT
GACCAAGAAATGCGTTACCGTCAGCGTTACGTTGACCTAATCGTGAACGAAGACTCTCGTAACACATTCATCGTGCGTTC
TAAGCTTGTGTCTTCAATCCGTAACTTCATGAGCTCAAAAGGCTACCTAGAAGTTGAAACGCCAATGATGCACGTGATCC
CAGGCGGTGCAACAGCACGTCCATTCATCACTCATCACAACGCATTAGACATCGACATGTACCTACGTGTTGCACCTGAG
CTTTACCTTAAGCGTTTAGTAGTTGGTGGTTTTGACCGTGTATTCGAGATCAACCGTAACTTCCGTAACGAAGGTCTTTC
TCCTCGTCATAACCCAGAGTTCACAATGATGGAATTCTACCAAGCGTACTCTGACTACAAAGATCTAATGGATCTAACGG
AAGAGATGCTAAGCACAGCGGCGATGGACGTTCTTGGTTCTACTTCTATGCCTTACGGCGACGAAACGGTTGAGTTCGGT
GGCACTTACGCTCGCATGAGCATGTTCGATGCAATCAAACACTACACACCTGAAAATGCAAACATTCAAGCTCTGACTGA
AGATGATCTTCAGAACAGAGAATTGATGGTTAAAATTGCAGAAGAAGTGGGTCTTTACGTTGAGAAGTTCTGGACATGTG
GTCAGCTTCTTGAAGAGATCTTTGGTGAAACGGCTGAGCCTCAGCTAATTCAACCAACGTTCATCACGGGTTACCCAGCG
GACATCTCTCCACTGGCTCGTCGTAGCGACAGCAACCCATTCTTCACAGACCGCTTTGAGTTCTTCATCGGTGGCCGTGA
AGTAGCGAACGGTTTCTCTGAGCTTAACGATGCACAAGACCAAGACGAGCGTTTCAAAGCACAAGTTAACGCAAAAGACG
CGGGTGATGACGAAGCTATGTACTACGATGCGGACTACATTACTGCACTAGAGCACGGCCTACCGCCAACAGCGGGTCAA
GGTATTGGTATCGATCGTCTAGCGATGCTATTTACTAACACGCACACAATCCGTGACGTGATCTTGTTCCCGGCAATGCG
CCCACAAGCGTAA

Upstream 100 bases:

>100_bases
AAGCTTTATCAACTATTAAGCTTTACCAATAATATTGGTAAAAATGCCTATATTTTCAAAAATATAGCTGTCCAAATTAT
AAAAGCAGGGTACATCTCAA

Downstream 100 bases:

>100_bases
TTTTCGCTTATGGGGTGTTACTGCCCGTGTAATTTAAAAGCCACCTTCGGGTGGCTTTTTCGGTTTTTAGCGTCAAAACC
TAGCTCGTACCGCACTCTTG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTDAVQNENTQEASSPEENKLIAERRSKLDHIRQNCKANGHPNDFRREHLAGDLQAEFGEKTKEELEELNHIVAIAGRIM
AKRGPFLAIQETSGRIQAYAAKDVQKVLKEKYQGLDIGDIIGVKGALHKSGKGDLYVNMEEFELLTKALRPLPEKFHGLT
DQEMRYRQRYVDLIVNEDSRNTFIVRSKLVSSIRNFMSSKGYLEVETPMMHVIPGGATARPFITHHNALDIDMYLRVAPE
LYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYQAYSDYKDLMDLTEEMLSTAAMDVLGSTSMPYGDETVEFG
GTYARMSMFDAIKHYTPENANIQALTEDDLQNRELMVKIAEEVGLYVEKFWTCGQLLEEIFGETAEPQLIQPTFITGYPA
DISPLARRSDSNPFFTDRFEFFIGGREVANGFSELNDAQDQDERFKAQVNAKDAGDDEAMYYDADYITALEHGLPPTAGQ
GIGIDRLAMLFTNTHTIRDVILFPAMRPQA

Sequences:

>Translated_510_residues
MTDAVQNENTQEASSPEENKLIAERRSKLDHIRQNCKANGHPNDFRREHLAGDLQAEFGEKTKEELEELNHIVAIAGRIM
AKRGPFLAIQETSGRIQAYAAKDVQKVLKEKYQGLDIGDIIGVKGALHKSGKGDLYVNMEEFELLTKALRPLPEKFHGLT
DQEMRYRQRYVDLIVNEDSRNTFIVRSKLVSSIRNFMSSKGYLEVETPMMHVIPGGATARPFITHHNALDIDMYLRVAPE
LYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYQAYSDYKDLMDLTEEMLSTAAMDVLGSTSMPYGDETVEFG
GTYARMSMFDAIKHYTPENANIQALTEDDLQNRELMVKIAEEVGLYVEKFWTCGQLLEEIFGETAEPQLIQPTFITGYPA
DISPLARRSDSNPFFTDRFEFFIGGREVANGFSELNDAQDQDERFKAQVNAKDAGDDEAMYYDADYITALEHGLPPTAGQ
GIGIDRLAMLFTNTHTIRDVILFPAMRPQA
>Mature_509_residues
TDAVQNENTQEASSPEENKLIAERRSKLDHIRQNCKANGHPNDFRREHLAGDLQAEFGEKTKEELEELNHIVAIAGRIMA
KRGPFLAIQETSGRIQAYAAKDVQKVLKEKYQGLDIGDIIGVKGALHKSGKGDLYVNMEEFELLTKALRPLPEKFHGLTD
QEMRYRQRYVDLIVNEDSRNTFIVRSKLVSSIRNFMSSKGYLEVETPMMHVIPGGATARPFITHHNALDIDMYLRVAPEL
YLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYQAYSDYKDLMDLTEEMLSTAAMDVLGSTSMPYGDETVEFGG
TYARMSMFDAIKHYTPENANIQALTEDDLQNRELMVKIAEEVGLYVEKFWTCGQLLEEIFGETAEPQLIQPTFITGYPAD
ISPLARRSDSNPFFTDRFEFFIGGREVANGFSELNDAQDQDERFKAQVNAKDAGDDEAMYYDADYITALEHGLPPTAGQG
IGIDRLAMLFTNTHTIRDVILFPAMRPQA

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=458, Percent_Identity=45.1965065502183, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI5031815, Length=458, Percent_Identity=45.1965065502183, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI45439306, Length=487, Percent_Identity=21.5605749486653, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI40789249, Length=276, Percent_Identity=25.3623188405797, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI226491502, Length=418, Percent_Identity=22.0095693779904, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789256, Length=496, Percent_Identity=62.7016129032258, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=497, Percent_Identity=61.1670020120724, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI87082379, Length=336, Percent_Identity=30.952380952381, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1788173, Length=255, Percent_Identity=25.8823529411765, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=509, Percent_Identity=40.2750491159136, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17535927, Length=509, Percent_Identity=40.2750491159136, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71994340, Length=509, Percent_Identity=40.2750491159136, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32566633, Length=267, Percent_Identity=27.3408239700375, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17551876, Length=357, Percent_Identity=25.4901960784314, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=517, Percent_Identity=41.9729206963249, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324256, Length=385, Percent_Identity=31.6883116883117, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6323011, Length=358, Percent_Identity=22.9050279329609, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24640849, Length=517, Percent_Identity=39.6518375241779, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640851, Length=517, Percent_Identity=39.6518375241779, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24584738, Length=306, Percent_Identity=26.4705882352941, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136276, Length=463, Percent_Identity=22.0302375809935, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57910; Mature: 57779

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAVQNENTQEASSPEENKLIAERRSKLDHIRQNCKANGHPNDFRREHLAGDLQAEFGE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHCH
KTKEELEELNHIVAIAGRIMAKRGPFLAIQETSGRIQAYAAKDVQKVLKEKYQGLDIGDI
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCHHHH
IGVKGALHKSGKGDLYVNMEEFELLTKALRPLPEKFHGLTDQEMRYRQRYVDLIVNEDSR
HCCCHHHCCCCCCCEEEEHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHEECCCCC
NTFIVRSKLVSSIRNFMSSKGYLEVETPMMHVIPGGATARPFITHHNALDIDMYLRVAPE
CEEEHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHH
LYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYQAYSDYKDLMDLTEEMLSTA
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMDVLGSTSMPYGDETVEFGGTYARMSMFDAIKHYTPENANIQALTEDDLQNRELMVKIA
HHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHH
EEVGLYVEKFWTCGQLLEEIFGETAEPQLIQPTFITGYPADISPLARRSDSNPFFTDRFE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCEEECCCCCCCCHHHHCCCCCCCCCCCHH
FFIGGREVANGFSELNDAQDQDERFKAQVNAKDAGDDEAMYYDADYITALEHGLPPTAGQ
HEECCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHCCCCCCCC
GIGIDRLAMLFTNTHTIRDVILFPAMRPQA
CCCHHHHHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDAVQNENTQEASSPEENKLIAERRSKLDHIRQNCKANGHPNDFRREHLAGDLQAEFGE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHCH
KTKEELEELNHIVAIAGRIMAKRGPFLAIQETSGRIQAYAAKDVQKVLKEKYQGLDIGDI
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCHHHH
IGVKGALHKSGKGDLYVNMEEFELLTKALRPLPEKFHGLTDQEMRYRQRYVDLIVNEDSR
HCCCHHHCCCCCCCEEEEHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHEECCCCC
NTFIVRSKLVSSIRNFMSSKGYLEVETPMMHVIPGGATARPFITHHNALDIDMYLRVAPE
CEEEHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHH
LYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYQAYSDYKDLMDLTEEMLSTA
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMDVLGSTSMPYGDETVEFGGTYARMSMFDAIKHYTPENANIQALTEDDLQNRELMVKIA
HHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHH
EEVGLYVEKFWTCGQLLEEIFGETAEPQLIQPTFITGYPADISPLARRSDSNPFFTDRFE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCEEECCCCCCCCHHHHCCCCCCCCCCCHH
FFIGGREVANGFSELNDAQDQDERFKAQVNAKDAGDDEAMYYDADYITALEHGLPPTAGQ
HEECCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHCCCCCCCC
GIGIDRLAMLFTNTHTIRDVILFPAMRPQA
CCCHHHHHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]