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The map label for this gene is prfB [H]

Identifier: 218708550

GI number: 218708550

Start: 534795

End: 535706

Strand: Direct

Name: prfB [H]

Synonym: VS_0516

Alternate gene names: 218708550

Gene position: 534795-535706 (Clockwise)

Preceding gene: 218708548

Following gene: 218708551

Centisome position: 16.21

GC content: 46.16

Gene sequence:

>912_bases
TTGGAAGCGGTAGTAGAAACGATCGACCAACTTGACCAAGGTGTTGAGGATGTTGATGGCCTACTAGAGCTTGCGGTTGA
AGAAGAAGATCAAGAAACGTTCGATGAAATTGAGCCAGAATTGGCCGAGCTTGAAGCTAAGTTAGAGAAGCTGGAATTCC
GTCGTATGTTCTCTGGTGATCACGACGCATCAGATTGCTACATCGATTTACAGTCAGGTTCGGGCGGTACAGAAGCTCAA
GACTGGACTTCAATGATGTTGCGTATGTACTTGCGTTGGGCAGATTCGAAAGGCTTCAAGACTGAAGTTATCGAAGTGTC
TGATGGCGATGTTGCTGGCCTTAAAGGCGCAACGGTACGTATCTCTGGTGAGTACGCTTACGGTTGGTTACGCACAGAGA
CTGGTGTTCACCGTCTAGTTCGTAAGTCACCATTTGATTCAAGTGGTCGTCGTCATACTTCATTTGCTTCTGCGTTTATC
TATCCTGAGATTGATGACAACATTACGATCGACATTAATCCTTCTGACCTACGTATTGACGTATACCGTGCCTCTGGCGC
TGGTGGTCAGCACGTAAACACCACGGAATCGGCGGTACGTATTACTCACGTTCCAACTAACACCGTGGTTCAGTGTCAGA
ATGACCGTTCGCAGCATAAGAACAAAGATCAAGCGATGAAGCAGCTACGTGCTAAGCTTTTTGAACTTGAGATTCAAAAG
CAAAATGCTGAAAAACAAGCGAGCGAAGAAACGAAATCAGACATCGGTTGGGGCAGTCAGATCCGCTCTTACGTGCTGGA
TGATTCACGTATCAAAGATTTACGCACCGGCATCGAAAACCGTAATACTCAAGCGGTTCTTGACGGTGACTTAGACAAGT
TTATTGAAGCTAGCCTGAAATCAGGTCTGTAA

Upstream 100 bases:

>100_bases
AAAGAAAGAGCGTCTAGAAGAAGTAAACGCAGAATTAGAACAACCGGATGTATGGAACGAACCTGAGCGTGCTCAAGCGC
TAGGAAAAGAGCGTTCAGCA

Downstream 100 bases:

>100_bases
GCTTTATCAACTATTAAGCTTTACCAATAATATTGGTAAAAATGCCTATATTTTCAAAAATATAGCTGTCCAAATTATAA
AAGCAGGGTACATCTCAAAT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MEAVVETIDQLDQGVEDVDGLLELAVEEEDQETFDEIEPELAELEAKLEKLEFRRMFSGDHDASDCYIDLQSGSGGTEAQ
DWTSMMLRMYLRWADSKGFKTEVIEVSDGDVAGLKGATVRISGEYAYGWLRTETGVHRLVRKSPFDSSGRRHTSFASAFI
YPEIDDNITIDINPSDLRIDVYRASGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELEIQK
QNAEKQASEETKSDIGWGSQIRSYVLDDSRIKDLRTGIENRNTQAVLDGDLDKFIEASLKSGL

Sequences:

>Translated_303_residues
MEAVVETIDQLDQGVEDVDGLLELAVEEEDQETFDEIEPELAELEAKLEKLEFRRMFSGDHDASDCYIDLQSGSGGTEAQ
DWTSMMLRMYLRWADSKGFKTEVIEVSDGDVAGLKGATVRISGEYAYGWLRTETGVHRLVRKSPFDSSGRRHTSFASAFI
YPEIDDNITIDINPSDLRIDVYRASGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELEIQK
QNAEKQASEETKSDIGWGSQIRSYVLDDSRIKDLRTGIENRNTQAVLDGDLDKFIEASLKSGL
>Mature_303_residues
MEAVVETIDQLDQGVEDVDGLLELAVEEEDQETFDEIEPELAELEAKLEKLEFRRMFSGDHDASDCYIDLQSGSGGTEAQ
DWTSMMLRMYLRWADSKGFKTEVIEVSDGDVAGLKGATVRISGEYAYGWLRTETGVHRLVRKSPFDSSGRRHTSFASAFI
YPEIDDNITIDINPSDLRIDVYRASGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELEIQK
QNAEKQASEETKSDIGWGSQIRSYVLDDSRIKDLRTGIENRNTQAVLDGDLDKFIEASLKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=303, Percent_Identity=35.973597359736, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI34577120, Length=318, Percent_Identity=32.3899371069182, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=37.6811594202899, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI2367172, Length=303, Percent_Identity=77.5577557755775, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=39.917695473251, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=252, Percent_Identity=34.9206349206349, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=34.4936708860759, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI19921226, Length=220, Percent_Identity=40, Blast_Score=161, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 34003; Mature: 34003

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAVVETIDQLDQGVEDVDGLLELAVEEEDQETFDEIEPELAELEAKLEKLEFRRMFSGD
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
HDASDCYIDLQSGSGGTEAQDWTSMMLRMYLRWADSKGFKTEVIEVSDGDVAGLKGATVR
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEE
ISGEYAYGWLRTETGVHRLVRKSPFDSSGRRHTSFASAFIYPEIDDNITIDINPSDLRID
EECCEEEEEEECHHHHHHHHHCCCCCCCCCCCHHHHHEEECCCCCCCEEEEECCCCEEEE
VYRASGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELEIQK
EEEECCCCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHH
QNAEKQASEETKSDIGWGSQIRSYVLDDSRIKDLRTGIENRNTQAVLDGDLDKFIEASLK
HCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHH
SGL
CCC
>Mature Secondary Structure
MEAVVETIDQLDQGVEDVDGLLELAVEEEDQETFDEIEPELAELEAKLEKLEFRRMFSGD
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
HDASDCYIDLQSGSGGTEAQDWTSMMLRMYLRWADSKGFKTEVIEVSDGDVAGLKGATVR
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEE
ISGEYAYGWLRTETGVHRLVRKSPFDSSGRRHTSFASAFIYPEIDDNITIDINPSDLRID
EECCEEEEEEECHHHHHHHHHCCCCCCCCCCCHHHHHEEECCCCCCCEEEEECCCCEEEE
VYRASGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELEIQK
EEEECCCCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHH
QNAEKQASEETKSDIGWGSQIRSYVLDDSRIKDLRTGIENRNTQAVLDGDLDKFIEASLK
HCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHH
SGL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA