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The map label for this gene is malX [H]

Identifier: 218708228

GI number: 218708228

Start: 160917

End: 162500

Strand: Reverse

Name: malX [H]

Synonym: VS_0165

Alternate gene names: 218708228

Gene position: 162500-160917 (Counterclockwise)

Preceding gene: 218708229

Following gene: 218708227

Centisome position: 4.93

GC content: 44.63

Gene sequence:

>1584_bases
ATGAGAGATAAGGTTCAAGCACTTGCTGGAGGTATGATGGTATCCATCATCGTACTGGTTATCGCAGGTATATTTATTGG
TGTGGGCGCTGGTGTTGTAAACCAAATGTCTGCTACCGACTCTGTTATTTGGGCATTATTTAAGCTGATGTTAGATCTTG
GCTTGATGGTCATGCGCAATCTACCGCCTTTCTTTGCGATTGGTCTAGCGTTCGCGCTGGCGAAATCTGAAAAAGGTTGG
GCAGCCTTCACTGGCTTTACCATGTTCATGTGTTTCAATGTCGCTATCGGATCGATCGCTGCATTTCACGGATGGACTCA
AGAAACTACAAGCGTTGCGTCATTAATGAATGATGCTGGCTACGACAAAGTAGCAGCGCAAAACTTCAACTCTTTATGGG
GTGAGTCTTTAGGTATCTTCGCTTACAACATGGGGATCTTTAGCGGTATTCTTGTTGGTTGCATTACGGCTTGGATCCAC
AACCGTTACTACAAATTCGAAATGCCTGCACTCTTGAGCTTCTTCTCTGGCCCTCGTGCCGTAGTGATTTTCGCTTACTT
CGCTATCATGCCTATCGCGCTGTTTGTTTACTACGCTTGGCCGCCGATCGCTGGGGCACTGCAGTCCATCACTACCTTGA
TTACTAGCTCAGGTATTTTTGGCTCTTTCCTATTTGGCGTATTTGACAAAGGCTTATTGCCATTTGGCTTACATCACCTT
ATCGCCTTCCCTATTGAATACACTCGTGTTGGTGGCGTAATGGAGATTGATGGTGTTGTTTACGAAGGTGTCCGTAACAT
CATGAATGGCCAAATGAGTTCACCAGAGGCGACGAGCTACATTACACATAACTTTACCAGTGGCCGTATTCCAATCCAAA
TTGGTGGCCTAACGGGTGCTGCTTATGCAATGTACGTAACAGCAAAAACAGCCAATCGTAAAAAGGTAGCCGCTATCATG
GTGCCTGCAGTATTTACGGCTGCTGTTATCGGTATTACCGAGCCACTAGAATATACCTTCCTATTCGTCCAACCATTCCT
ATTCTTTGCAGTACATGTTCCGCTAAATGGTCTTGCTTATGTGATCACCGAAATGATGGGTGTTTCAATTCACGGAAATC
AGCTGCTATTTATGGTGCCTAACCTACTACAACCTGAAAAGGTTCATGCTTGGGCTCTATTGTGGATTATCCCACTATAC
TTCGCGATTTACTTCTACACATTCAAGTTCTTCATCCTGAAGTTTGATTCAAAAACTCCAGGTCGCGAAGAAGAAGATGG
TGATATCGCCCTGTATTCAAAAGAAGACTTTAAAAAGAAAAAAGAGCAGACCTCAAAAGGACTGCCTGTTGAAATCATTA
AAGCGCTGGGTGGGGCTGACAACATTGAGAACGTGACAAACTGTGCAACTCGACTGCGTGTTTCGCTGCATGACGAATCA
CTAACGGCAAGCGATAAAGTTTGGAAACAACAGCTCAACGCAATTGGCGTCGTCAGAATGAATAAAGGCATTCAAGTGAT
ATATGGAGCCAACGTTATTACGATTGCCTCTGGTATCAAAGAAGCCCTTGGTGTCGACTCTTAG

Upstream 100 bases:

>100_bases
GACTCGTGTCCATGAGCCCAATGAAAGGCTCAGCCTCGAGTCATTAAAAAGAGCAATAACAATATACGTGAAAGTTCTCA
TATCACTTTAGGAGTAAATT

Downstream 100 bases:

>100_bases
CTAAGCGCAACTTCCGTAACCCTACTGTTACGAATCTATAGAAACCAATAACTGACAATTCCTCCTGTGACTACAAGTCA
CAGGAAGGGATTCGTGCACC

Product: putative PTS system maltose and glucose-specific transporter subunits IIABC

Products: NA

Alternate protein names: Maltose and glucose permease IIC component; PTS system maltose- and glucose-specific EIIC component; Maltose- and glucose-specific phosphotransferase enzyme IIB component; PTS system maltose- and glucose-specific EIIB component [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MRDKVQALAGGMMVSIIVLVIAGIFIGVGAGVVNQMSATDSVIWALFKLMLDLGLMVMRNLPPFFAIGLAFALAKSEKGW
AAFTGFTMFMCFNVAIGSIAAFHGWTQETTSVASLMNDAGYDKVAAQNFNSLWGESLGIFAYNMGIFSGILVGCITAWIH
NRYYKFEMPALLSFFSGPRAVVIFAYFAIMPIALFVYYAWPPIAGALQSITTLITSSGIFGSFLFGVFDKGLLPFGLHHL
IAFPIEYTRVGGVMEIDGVVYEGVRNIMNGQMSSPEATSYITHNFTSGRIPIQIGGLTGAAYAMYVTAKTANRKKVAAIM
VPAVFTAAVIGITEPLEYTFLFVQPFLFFAVHVPLNGLAYVITEMMGVSIHGNQLLFMVPNLLQPEKVHAWALLWIIPLY
FAIYFYTFKFFILKFDSKTPGREEEDGDIALYSKEDFKKKKEQTSKGLPVEIIKALGGADNIENVTNCATRLRVSLHDES
LTASDKVWKQQLNAIGVVRMNKGIQVIYGANVITIASGIKEALGVDS

Sequences:

>Translated_527_residues
MRDKVQALAGGMMVSIIVLVIAGIFIGVGAGVVNQMSATDSVIWALFKLMLDLGLMVMRNLPPFFAIGLAFALAKSEKGW
AAFTGFTMFMCFNVAIGSIAAFHGWTQETTSVASLMNDAGYDKVAAQNFNSLWGESLGIFAYNMGIFSGILVGCITAWIH
NRYYKFEMPALLSFFSGPRAVVIFAYFAIMPIALFVYYAWPPIAGALQSITTLITSSGIFGSFLFGVFDKGLLPFGLHHL
IAFPIEYTRVGGVMEIDGVVYEGVRNIMNGQMSSPEATSYITHNFTSGRIPIQIGGLTGAAYAMYVTAKTANRKKVAAIM
VPAVFTAAVIGITEPLEYTFLFVQPFLFFAVHVPLNGLAYVITEMMGVSIHGNQLLFMVPNLLQPEKVHAWALLWIIPLY
FAIYFYTFKFFILKFDSKTPGREEEDGDIALYSKEDFKKKKEQTSKGLPVEIIKALGGADNIENVTNCATRLRVSLHDES
LTASDKVWKQQLNAIGVVRMNKGIQVIYGANVITIASGIKEALGVDS
>Mature_527_residues
MRDKVQALAGGMMVSIIVLVIAGIFIGVGAGVVNQMSATDSVIWALFKLMLDLGLMVMRNLPPFFAIGLAFALAKSEKGW
AAFTGFTMFMCFNVAIGSIAAFHGWTQETTSVASLMNDAGYDKVAAQNFNSLWGESLGIFAYNMGIFSGILVGCITAWIH
NRYYKFEMPALLSFFSGPRAVVIFAYFAIMPIALFVYYAWPPIAGALQSITTLITSSGIFGSFLFGVFDKGLLPFGLHHL
IAFPIEYTRVGGVMEIDGVVYEGVRNIMNGQMSSPEATSYITHNFTSGRIPIQIGGLTGAAYAMYVTAKTANRKKVAAIM
VPAVFTAAVIGITEPLEYTFLFVQPFLFFAVHVPLNGLAYVITEMMGVSIHGNQLLFMVPNLLQPEKVHAWALLWIIPLY
FAIYFYTFKFFILKFDSKTPGREEEDGDIALYSKEDFKKKKEQTSKGLPVEIIKALGGADNIENVTNCATRLRVSLHDES
LTASDKVWKQQLNAIGVVRMNKGIQVIYGANVITIASGIKEALGVDS

Specific function: MalX encodes a phosphotransferase system enzyme II that can recognize glucose and maltose as substrates even though these sugars may not represent the natural substrates of the system [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787908, Length=523, Percent_Identity=32.3135755258126, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1787343, Length=528, Percent_Identity=29.3560606060606, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1786894, Length=384, Percent_Identity=33.0729166666667, Blast_Score=182, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011301 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 57664; Mature: 57664

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHEEEEEECCCEEEEECCCCEEHHHHHHHHHCCCC
>Mature Secondary Structure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CCHHHHHHHHHHHHEEEEEECCCEEEEECCCCEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1856179; 9097039; 9278503; 2670898 [H]