Click here to switch to the map view.

The map label for this gene is envZ [H]

Identifier: 218708220

GI number: 218708220

Start: 149898

End: 151193

Strand: Direct

Name: envZ [H]

Synonym: VS_0157

Alternate gene names: 218708220

Gene position: 149898-151193 (Clockwise)

Preceding gene: 218708219

Following gene: 218708225

Centisome position: 4.54

GC content: 48.92

Gene sequence:

>1296_bases
ATGCGCATACGTAGCTCCTTTACTCAGTCGATAGTGCTCTTCTTAACCCTGTTGGTTGCCAGCCAAATTTTTTCGTATTA
CGCGGTGTTCAATTATGCATTGATGCCGAGTTTGCAGCAGTTTAATAAGATTTTGGCTCACGAACTCAACCTAGTATTGG
ACCAAGGCAGTGATATCGAGATTGATGCGCCACTGCGTCAGCGTGTACTTGAGCAGCTCGGGGTTACGGTTCATGCAAAG
GACAGTGAAGCGTCGGGCGAGTACTACCATGCGGTCGCGATAGACCTGATGAGTGAGGAGATGACCAAAGAGTTGGGCTC
TGAGACCGAAGTGCGATTGGTTTTGGGCAAAGAAAGTTACGTGTTGTGGATGGACATTGCTCAGTTGCCTAACTCTTTGA
TTCGTATTCCCCTGTCTGAGCTGCAAGAAGAAGACTTTGCGCCTCTATTTCGTAACAGTCTGATCATGGCGTTGTTGATT
GTTGCTGGTGGGTGGCTGTTTATCCGCTTGCAAAATCGGCCGTTGATAGCCCTAGAGAAAGCCGCGCAAGGTGTTGGGCG
AGGTGATATTCCACCGCCCTTACCAGTACAAGGTGCACAAGAGATTCGCTCGGTAACTCGAGCCTTTAATCAGATGTCGA
AAGGCATTCAAGAACTCGAAGAAGATAGAGCCCTACTAATGGCGGGTATCAGCCATGATCTGCGTACACCACTCACGCGT
ATTCGCTTGGCGACTGAGATGATGTCGCCAGAAGATAGCTATCTAGCGGAAGGGATCATCAGTGATACTGAAGAGTGCAA
TGAGATCATCAGTCAGTTTATGGATTACCTAAAGCCTGTGGACCGAGACTCATTCCAGGCGGTATTTGTCGATGATATTG
CGCGCGAGGTCTCTAGCTCTGAAGGAGGCTATGAGGTTCAGATTGAAACCGATATTCCAGAAACCATGAAACCTGCTTTG
GGTAACCCGATCGCGATGAAGCGTGCGGTGAGTAATTTGGTGGTAAATGCGCTGCGCTACGGTAATGGTTGGGTCAAAGT
ATCGACTGGCATGACAGCAGATAACAAACTGGCTTGGGTAACGGTAGAAGATAATGGTCCGGGTATTCCACAAGACCAAA
TTGGTAAGTTGTTTGAACCGTTCACTCGTGGTGACACGGCTCGTGGTAGTGAAGGGACAGGTTTAGGTTTAGCGATCGTG
AAACGTATTGTCAGCCAACACCAAGGTGCGGTGGTGGTGAATAACCGCAGTGAGGGTGGCTTGAAGGCGCAGATCAGTTT
CCCTGTGAAACCTTAG

Upstream 100 bases:

>100_bases
CCTTTTTCCGGCGCTATTTATGATCATTTACTTACTGTGATTGGTATTACAGCTATAGTTCCCACTATATTCCTTTATCA
TCCCTCACTTTAGGTCTTCT

Downstream 100 bases:

>100_bases
GCTATACGCACGAAAAAGGCTTCCTTGTAGAAGCCTTTTTACGTTTTAAGCAAGATATGAGGGCGAATTACGCCTTAGAA
TACACATCGCGCTCACCCAG

Product: osmolarity sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MRIRSSFTQSIVLFLTLLVASQIFSYYAVFNYALMPSLQQFNKILAHELNLVLDQGSDIEIDAPLRQRVLEQLGVTVHAK
DSEASGEYYHAVAIDLMSEEMTKELGSETEVRLVLGKESYVLWMDIAQLPNSLIRIPLSELQEEDFAPLFRNSLIMALLI
VAGGWLFIRLQNRPLIALEKAAQGVGRGDIPPPLPVQGAQEIRSVTRAFNQMSKGIQELEEDRALLMAGISHDLRTPLTR
IRLATEMMSPEDSYLAEGIISDTEECNEIISQFMDYLKPVDRDSFQAVFVDDIAREVSSSEGGYEVQIETDIPETMKPAL
GNPIAMKRAVSNLVVNALRYGNGWVKVSTGMTADNKLAWVTVEDNGPGIPQDQIGKLFEPFTRGDTARGSEGTGLGLAIV
KRIVSQHQGAVVVNNRSEGGLKAQISFPVKP

Sequences:

>Translated_431_residues
MRIRSSFTQSIVLFLTLLVASQIFSYYAVFNYALMPSLQQFNKILAHELNLVLDQGSDIEIDAPLRQRVLEQLGVTVHAK
DSEASGEYYHAVAIDLMSEEMTKELGSETEVRLVLGKESYVLWMDIAQLPNSLIRIPLSELQEEDFAPLFRNSLIMALLI
VAGGWLFIRLQNRPLIALEKAAQGVGRGDIPPPLPVQGAQEIRSVTRAFNQMSKGIQELEEDRALLMAGISHDLRTPLTR
IRLATEMMSPEDSYLAEGIISDTEECNEIISQFMDYLKPVDRDSFQAVFVDDIAREVSSSEGGYEVQIETDIPETMKPAL
GNPIAMKRAVSNLVVNALRYGNGWVKVSTGMTADNKLAWVTVEDNGPGIPQDQIGKLFEPFTRGDTARGSEGTGLGLAIV
KRIVSQHQGAVVVNNRSEGGLKAQISFPVKP
>Mature_431_residues
MRIRSSFTQSIVLFLTLLVASQIFSYYAVFNYALMPSLQQFNKILAHELNLVLDQGSDIEIDAPLRQRVLEQLGVTVHAK
DSEASGEYYHAVAIDLMSEEMTKELGSETEVRLVLGKESYVLWMDIAQLPNSLIRIPLSELQEEDFAPLFRNSLIMALLI
VAGGWLFIRLQNRPLIALEKAAQGVGRGDIPPPLPVQGAQEIRSVTRAFNQMSKGIQELEEDRALLMAGISHDLRTPLTR
IRLATEMMSPEDSYLAEGIISDTEECNEIISQFMDYLKPVDRDSFQAVFVDDIAREVSSSEGGYEVQIETDIPETMKPAL
GNPIAMKRAVSNLVVNALRYGNGWVKVSTGMTADNKLAWVTVEDNGPGIPQDQIGKLFEPFTRGDTARGSEGTGLGLAIV
KRIVSQHQGAVVVNNRSEGGLKAQISFPVKP

Specific function: Member of the two-component regulatory system envZ/ompR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates ompR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789808, Length=433, Percent_Identity=48.2678983833718, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1790346, Length=270, Percent_Identity=31.1111111111111, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787894, Length=275, Percent_Identity=28.7272727272727, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786783, Length=294, Percent_Identity=27.891156462585, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788393, Length=275, Percent_Identity=26.1818181818182, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082128, Length=296, Percent_Identity=25.3378378378378, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786912, Length=250, Percent_Identity=27.6, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788549, Length=218, Percent_Identity=27.5229357798165, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693157, Length=241, Percent_Identity=28.2157676348548, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790436, Length=214, Percent_Identity=26.1682242990654, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790861, Length=282, Percent_Identity=26.241134751773, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786600, Length=248, Percent_Identity=28.2258064516129, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789149, Length=232, Percent_Identity=28.0172413793103, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787374, Length=292, Percent_Identity=26.7123287671233, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081816, Length=331, Percent_Identity=23.8670694864048, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788279, Length=257, Percent_Identity=25.6809338521401, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789403, Length=302, Percent_Identity=27.1523178807947, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI48994928, Length=220, Percent_Identity=27.7272727272727, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47636; Mature: 47636

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIRSSFTQSIVLFLTLLVASQIFSYYAVFNYALMPSLQQFNKILAHELNLVLDQGSDIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEE
IDAPLRQRVLEQLGVTVHAKDSEASGEYYHAVAIDLMSEEMTKELGSETEVRLVLGKESY
ECCHHHHHHHHHHCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCE
VLWMDIAQLPNSLIRIPLSELQEEDFAPLFRNSLIMALLIVAGGWLFIRLQNRPLIALEK
EEEEEHHHCCHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHH
AAQGVGRGDIPPPLPVQGAQEIRSVTRAFNQMSKGIQELEEDRALLMAGISHDLRTPLTR
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IRLATEMMSPEDSYLAEGIISDTEECNEIISQFMDYLKPVDRDSFQAVFVDDIAREVSSS
HHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
EGGYEVQIETDIPETMKPALGNPIAMKRAVSNLVVNALRYGNGWVKVSTGMTADNKLAWV
CCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEE
TVEDNGPGIPQDQIGKLFEPFTRGDTARGSEGTGLGLAIVKRIVSQHQGAVVVNNRSEGG
EEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
LKAQISFPVKP
EEEEEECCCCC
>Mature Secondary Structure
MRIRSSFTQSIVLFLTLLVASQIFSYYAVFNYALMPSLQQFNKILAHELNLVLDQGSDIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEE
IDAPLRQRVLEQLGVTVHAKDSEASGEYYHAVAIDLMSEEMTKELGSETEVRLVLGKESY
ECCHHHHHHHHHHCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCE
VLWMDIAQLPNSLIRIPLSELQEEDFAPLFRNSLIMALLIVAGGWLFIRLQNRPLIALEK
EEEEEHHHCCHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHH
AAQGVGRGDIPPPLPVQGAQEIRSVTRAFNQMSKGIQELEEDRALLMAGISHDLRTPLTR
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IRLATEMMSPEDSYLAEGIISDTEECNEIISQFMDYLKPVDRDSFQAVFVDDIAREVSSS
HHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
EGGYEVQIETDIPETMKPALGNPIAMKRAVSNLVVNALRYGNGWVKVSTGMTADNKLAWV
CCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEE
TVEDNGPGIPQDQIGKLFEPFTRGDTARGSEGTGLGLAIVKRIVSQHQGAVVVNNRSEGG
EEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
LKAQISFPVKP
EEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6292200; 2997120; 9278503; 8132603; 2277041; 2824492; 1323560; 9817206; 10426948 [H]