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The map label for this gene is pbuG [H]

Identifier: 218708225

GI number: 218708225

Start: 157348

End: 158718

Strand: Direct

Name: pbuG [H]

Synonym: VS_0162

Alternate gene names: 218708225

Gene position: 157348-158718 (Clockwise)

Preceding gene: 218708220

Following gene: 218708226

Centisome position: 4.77

GC content: 46.1

Gene sequence:

>1371_bases
GTGAATACCGACTCCACCCTGAAAGCCCAGAAGGCATCTGGCTCACTTGACACCCTGTTCAAACTCTCAGAGAGAAAGAC
CACGATCAGCACTGAGCTATACGCAGGTTTTATTACCTTCTTAGCGATGAGTTATATCTTGGCGGTTAACCCAGCAATTT
TGGGTGGCATTCCGGGAATGGACAAAGGTGCAGTCTTTACGGCGACCGCTCTTTCTGCTGCTATTGCAACGCTTATCATG
GGCATTTGGGGTAACTATCCAGTCATGCTAGCACCTGGCATGAGCATGAATGGCTTCTTCAAAGGTTTATTGTTAAGTGG
TTCAGTGGCGGTACTTTGGAATGAAGCATTATTTGGCATCTTCCTATCGGGTATTCTTTATCTTGCCTTCTCATTGACTA
ATATCCGTAAGTCGATGATTGAATCGATTCCTGAAGATTTGAAGCTGGCGATTACTGTGTCTCTCGGCTTGTTCATTGCT
TTCTTAGGCCTTAAGAATGCGGGTATCATCGTTTCTAACCCGTTCGTACTGGTTGGTTTAGGTGACATTTCCGATCCAAA
AGTGATCATTGCTTATGTGAGCATCTTCATCGCATTGGGCTGTATGATTCGTGATATTAAGTTGGCCACGTTTATCTCGT
TTGTTTCAGCTATCGTGTTGACCATTCTTGCTGATGTATTCATGGGCACATCAAACGCCCCAATCCCTGACCAATTCGTT
TCAATGCCACCAAGTATGGCAGGCAGCTTTGGCGCTATCTTTGACTTCTCAGCTTTTACGCCTGAGAAAATGTTCGATTT
GTTGTTCATCGTACTTATCTTCTTGATTGTCGATTTCTTCGATGGATTGAGCACGATTGTGGGTGTTGGCCGTGATGCAG
GTATCATCGACAAAGACGGTAAAGTGCCGAATGCAAAATCGGCGCTAGTGGCTGATGCGGGCGGTACTGTGATTGGTTCA
ATTCTAGGTACCACATCGATTACTGCTTTCTCTGAATCGGGTATTGCCTCTTCTCAAGGTGCGAAAACAGGTTTAGCGGC
AGTCATGGTGGCTGGCTTATTCCTAGTGTCTCTATTCCTATACCCAATCTTCTCTATCTTCTCAGCGGCAATGGTCGCGC
CTGCGATGGTTGTGGTAGGCATCTACATGGTGGGTCGTCTTGGTCAGATTAACTGGGAAAAGAAAGAGTCACGCATTGCA
GCTTTCTTCACCATCATGTTCACAGTGCTAAGCTTCTCACCAGCGAACGGTATGGCGATGGGTTTCATCAGCTACGCATT
CACAATGGTTGTTGCAGGTAAGCGCAAAGAAGTGCACCCATTGATCTACGGGCTGTGTGTGGTGTTCTTAACGTACCTGA
TTCTGTTGTAA

Upstream 100 bases:

>100_bases
ACCGAGCCTACCTTGGTGACTACAGTTTTTAGTGTAGAGATCCCTCTATTCTCACAGCATTTGAATCTCAAATTTCTTAA
TTTAATACATAGGTTTTGTT

Downstream 100 bases:

>100_bases
GGTTAGTTAGCCACTAGAAAGTGGCTAAGTAAAAAAACAAGTCGATGGCTCTGCAGTGAATGTGGGGCCATTTTTGTATT
CGTCGCTTCTCTAGAGCGCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MNTDSTLKAQKASGSLDTLFKLSERKTTISTELYAGFITFLAMSYILAVNPAILGGIPGMDKGAVFTATALSAAIATLIM
GIWGNYPVMLAPGMSMNGFFKGLLLSGSVAVLWNEALFGIFLSGILYLAFSLTNIRKSMIESIPEDLKLAITVSLGLFIA
FLGLKNAGIIVSNPFVLVGLGDISDPKVIIAYVSIFIALGCMIRDIKLATFISFVSAIVLTILADVFMGTSNAPIPDQFV
SMPPSMAGSFGAIFDFSAFTPEKMFDLLFIVLIFLIVDFFDGLSTIVGVGRDAGIIDKDGKVPNAKSALVADAGGTVIGS
ILGTTSITAFSESGIASSQGAKTGLAAVMVAGLFLVSLFLYPIFSIFSAAMVAPAMVVVGIYMVGRLGQINWEKKESRIA
AFFTIMFTVLSFSPANGMAMGFISYAFTMVVAGKRKEVHPLIYGLCVVFLTYLILL

Sequences:

>Translated_456_residues
MNTDSTLKAQKASGSLDTLFKLSERKTTISTELYAGFITFLAMSYILAVNPAILGGIPGMDKGAVFTATALSAAIATLIM
GIWGNYPVMLAPGMSMNGFFKGLLLSGSVAVLWNEALFGIFLSGILYLAFSLTNIRKSMIESIPEDLKLAITVSLGLFIA
FLGLKNAGIIVSNPFVLVGLGDISDPKVIIAYVSIFIALGCMIRDIKLATFISFVSAIVLTILADVFMGTSNAPIPDQFV
SMPPSMAGSFGAIFDFSAFTPEKMFDLLFIVLIFLIVDFFDGLSTIVGVGRDAGIIDKDGKVPNAKSALVADAGGTVIGS
ILGTTSITAFSESGIASSQGAKTGLAAVMVAGLFLVSLFLYPIFSIFSAAMVAPAMVVVGIYMVGRLGQINWEKKESRIA
AFFTIMFTVLSFSPANGMAMGFISYAFTMVVAGKRKEVHPLIYGLCVVFLTYLILL
>Mature_456_residues
MNTDSTLKAQKASGSLDTLFKLSERKTTISTELYAGFITFLAMSYILAVNPAILGGIPGMDKGAVFTATALSAAIATLIM
GIWGNYPVMLAPGMSMNGFFKGLLLSGSVAVLWNEALFGIFLSGILYLAFSLTNIRKSMIESIPEDLKLAITVSLGLFIA
FLGLKNAGIIVSNPFVLVGLGDISDPKVIIAYVSIFIALGCMIRDIKLATFISFVSAIVLTILADVFMGTSNAPIPDQFV
SMPPSMAGSFGAIFDFSAFTPEKMFDLLFIVLIFLIVDFFDGLSTIVGVGRDAGIIDKDGKVPNAKSALVADAGGTVIGS
ILGTTSITAFSESGIASSQGAKTGLAAVMVAGLFLVSLFLYPIFSIFSAAMVAPAMVVVGIYMVGRLGQINWEKKESRIA
AFFTIMFTVLSFSPANGMAMGFISYAFTMVVAGKRKEVHPLIYGLCVVFLTYLILL

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082309, Length=456, Percent_Identity=37.0614035087719, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1790150, Length=456, Percent_Identity=36.6228070175439, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1790499, Length=440, Percent_Identity=29.0909090909091, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI48994909, Length=443, Percent_Identity=27.9909706546275, Blast_Score=156, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 48270; Mature: 48270

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTDSTLKAQKASGSLDTLFKLSERKTTISTELYAGFITFLAMSYILAVNPAILGGIPGM
CCCCCCCHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCC
DKGAVFTATALSAAIATLIMGIWGNYPVMLAPGMSMNGFFKGLLLSGSVAVLWNEALFGI
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHH
FLSGILYLAFSLTNIRKSMIESIPEDLKLAITVSLGLFIAFLGLKNAGIIVSNPFVLVGL
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCEEEECCEEEEEC
GDISDPKVIIAYVSIFIALGCMIRDIKLATFISFVSAIVLTILADVFMGTSNAPIPDQFV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SMPPSMAGSFGAIFDFSAFTPEKMFDLLFIVLIFLIVDFFDGLSTIVGVGRDAGIIDKDG
CCCCHHCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KVPNAKSALVADAGGTVIGSILGTTSITAFSESGIASSQGAKTGLAAVMVAGLFLVSLFL
CCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YPIFSIFSAAMVAPAMVVVGIYMVGRLGQINWEKKESRIAAFFTIMFTVLSFSPANGMAM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
GFISYAFTMVVAGKRKEVHPLIYGLCVVFLTYLILL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNTDSTLKAQKASGSLDTLFKLSERKTTISTELYAGFITFLAMSYILAVNPAILGGIPGM
CCCCCCCHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCC
DKGAVFTATALSAAIATLIMGIWGNYPVMLAPGMSMNGFFKGLLLSGSVAVLWNEALFGI
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHH
FLSGILYLAFSLTNIRKSMIESIPEDLKLAITVSLGLFIAFLGLKNAGIIVSNPFVLVGL
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCEEEECCEEEEEC
GDISDPKVIIAYVSIFIALGCMIRDIKLATFISFVSAIVLTILADVFMGTSNAPIPDQFV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SMPPSMAGSFGAIFDFSAFTPEKMFDLLFIVLIFLIVDFFDGLSTIVGVGRDAGIIDKDG
CCCCHHCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KVPNAKSALVADAGGTVIGSILGTTSITAFSESGIASSQGAKTGLAAVMVAGLFLVSLFL
CCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YPIFSIFSAAMVAPAMVVVGIYMVGRLGQINWEKKESRIAAFFTIMFTVLSFSPANGMAM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
GFISYAFTMVVAGKRKEVHPLIYGLCVVFLTYLILL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377; 11591660 [H]