Click here to switch to the map view.

The map label for this gene is sgbE

Identifier: 218702349

GI number: 218702349

Start: 4264998

End: 4265693

Strand: Direct

Name: sgbE

Synonym: ECIAI39_4095

Alternate gene names: 218702349

Gene position: 4264998-4265693 (Clockwise)

Preceding gene: 218702348

Following gene: 218702351

Centisome position: 83.1

GC content: 57.33

Gene sequence:

>696_bases
ATGTTAGAGCAACTAAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGTTCATCATCTGGTGACGTTCACCTGGGG
CAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTACAACGTGATGACCGCCG
ACGATATGGTAGTGGTTGAGATAGCCACCGGTAAGGTGGTGGAAGGCAGAAAAAAACCCTCGTCCGACACGCCAACGCAT
CTGGCGCTTTACCGTCGCTATGCCGAAATTGGCGGTATTGTGCATACCCACTCGCGCCACGCCACCATCTGGTCGCAGGC
CGGGCTGGATCTCCCCGCCTGGGGCACCACGCACGCTGATTACTTCTATGGTGCCATCCCCTGCACGCGACAGATGACCG
CAGAGGAGATCAACGGCGAGTATGAATACCAGACCGGCGAAGTGATCATTAAAACCTTCGAAGAACGTGGGTTGAACCCG
GCACAAATCCCGGCGGTACTGGTGCATTCTCACGGCCCGTTCGCCTGGGGAAAAAACGCCGCCGATGCGGTGCATAACGC
CGTGGTGCTGGAAGAGTGCGCCTATATGGGCCTGTTCTCGCACCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAAC
TGCTGGATAAGCACTACCTGCGTAAACATGGGACCAATGCCTATTACGGGCAGTAA

Upstream 100 bases:

>100_bases
TTCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGCTGGATTGAAGCGCGT
ATGCAGGAGGCCGGATTTAT

Downstream 100 bases:

>100_bases
TCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGC
GTTAAACCAGTGTGTTTGCG

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: Phosphoribulose isomerase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MLEQLKADVLAANLALPVHHLVTFTWGNVSAVDETRQWMVIKPSGVEYNVMTADDMVVVEIATGKVVEGRKKPSSDTPTH
LALYRRYAEIGGIVHTHSRHATIWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIKTFEERGLNP
AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSHQLAPQLPAMQNELLDKHYLRKHGTNAYYGQ

Sequences:

>Translated_231_residues
MLEQLKADVLAANLALPVHHLVTFTWGNVSAVDETRQWMVIKPSGVEYNVMTADDMVVVEIATGKVVEGRKKPSSDTPTH
LALYRRYAEIGGIVHTHSRHATIWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIKTFEERGLNP
AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSHQLAPQLPAMQNELLDKHYLRKHGTNAYYGQ
>Mature_231_residues
MLEQLKADVLAANLALPVHHLVTFTWGNVSAVDETRQWMVIKPSGVEYNVMTADDMVVVEIATGKVVEGRKKPSSDTPTH
LALYRRYAEIGGIVHTHSRHATIWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIKTFEERGLNP
AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSHQLAPQLPAMQNELLDKHYLRKHGTNAYYGQ

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. May be involved in the utilization of 2,3- diketo-L-gulonate [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790008, Length=231, Percent_Identity=96.1038961038961, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI1786247, Length=231, Percent_Identity=75.3246753246753, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1790642, Length=230, Percent_Identity=60, Blast_Score=286, Evalue=1e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR004661 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 25665; Mature: 25665

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQLKADVLAANLALPVHHLVTFTWGNVSAVDETRQWMVIKPSGVEYNVMTADDMVVVE
CCHHHHHHHHHHHHHHHHHHEEEEECCCEEECCCCCCEEEECCCCCEEEEEECCCEEEEE
IATGKVVEGRKKPSSDTPTHLALYRRYAEIGGIVHTHSRHATIWSQAGLDLPAWGTTHAD
EECCCEECCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCEEEHHHCCCCCCCCCCCCCC
YFYGAIPCTRQMTAEEINGEYEYQTGEVIIKTFEERGLNPAQIPAVLVHSHGPFAWGKNA
CEEECCCCCHHHHHHHCCCEEEEECCHHHEEEHHHCCCCHHHCCEEEEECCCCCCCCCCH
ADAVHNAVVLEECAYMGLFSHQLAPQLPAMQNELLDKHYLRKHGTNAYYGQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLEQLKADVLAANLALPVHHLVTFTWGNVSAVDETRQWMVIKPSGVEYNVMTADDMVVVE
CCHHHHHHHHHHHHHHHHHHEEEEECCCEEECCCCCCEEEECCCCCEEEEEECCCEEEEE
IATGKVVEGRKKPSSDTPTHLALYRRYAEIGGIVHTHSRHATIWSQAGLDLPAWGTTHAD
EECCCEECCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCEEEHHHCCCCCCCCCCCCCC
YFYGAIPCTRQMTAEEINGEYEYQTGEVIIKTFEERGLNPAQIPAVLVHSHGPFAWGKNA
CEEECCCCCHHHHHHHCCCEEEEECCHHHEEEHHHCCCCHHHCCEEEEECCCCCCCCCCH
ADAVHNAVVLEECAYMGLFSHQLAPQLPAMQNELLDKHYLRKHGTNAYYGQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 11741871 [H]