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The map label for this gene is yflS [H]

Identifier: 218699731

GI number: 218699731

Start: 1418908

End: 1420314

Strand: Direct

Name: yflS [H]

Synonym: ECIAI39_1352

Alternate gene names: 218699731

Gene position: 1418908-1420314 (Clockwise)

Preceding gene: 218699730

Following gene: 218699732

Centisome position: 27.65

GC content: 51.95

Gene sequence:

>1407_bases
TTGCATATGAGTTGCTATTTGAAAGTCCTGATTTGTGCGATTGTCGCACTCGCTATATGGTTTTTCCCCGTCCCCGACGG
ATTAACACCCTTAACCTGGCACATTCTGGCCATATTTATCACCACTGTGGTGGCGTTTATTCTGCAACCGTTGCCGGTGG
GCGCTATCGCGTTAATAGCCATCAGTTTTATTATGCTGACCGGTATGATGAAAACCAGCGAAGCCTTAAAAGGTTTCAGC
AGCACGACGGTATGGCTTATTGTCGCCGCATTCCTGTACGCGAAAGGATTCATCAAAACGGGGTTGGGGCGGCGTATCGC
CTATTTGCTGATTCGCGGATTTGGCGGCAGCTCGTTGCGTCTGGGTTATACATTGGCGCTAAGCGATATGATTATCGCGC
CCGCAACGCCATCAAACACAGCGCGAGCAGGAGGGATTTTGTTCCCGATTGTTCGCAGTGTCTCTAACAGCTTTGGATCT
GAACCGGATCAAGGGCCGCGTAAAATAGGCGCTTATCTGATGCAGACGGTGTTCCATTCCAACTGCCTCTCATCCTCGAT
GTTTATGACGGCCAGCGCGCCAAATGCACTCATTGTATCTCTGGCGGCCAGCACTTTATTTGTCGATATCTCATGGGGGA
TGTGGACGCTAAGCGCACTGGTGCCGGGCATTATCGCTTTTATCACTATGCCGTTGGTCATTTACAAACTCTACCCGCCA
GAGATCAAAAAGACGCCAGAAGCCAAAGCGTTGGCACAGGAAGAACTTATCAAAATGGGGCCGGTAACCCGTGATGAGCG
CGTGACGATTGGCATTTTCCTGCTTTCATTATTGGCCTGGAGTACATCGAAATGGACTGGCCTGGATGCGACGGCTGTCG
CGCTTTCTGGCGTCTGTTTAATGTTGATGACCCGGATTATCACCTGGCAGGATGTGCAGTCAGAGAAGGGGGCCTGGGAC
ATTCTGGTCTGGCTGGGAGTCATGATCTGTATGGCAGATAAGCTCAATCAGCTTGGCCTGTTCAAATGGTTTGCCGTCAC
AACCTCGGCGCTGTTCACCGGCATTCCGTGGGAGATAACGTTGACGGTGTTGCTGATAGTTTACTGCTATTCCCACTATT
TCTTTGCCGGAAGTACGCCGCATGTAGTGGCGATGTATGCCGCTTTTGGCAGCGTCTCGGTTGCTGCTGGCGCACCGCCG
ATGATGGCGGCTCTGTCACTGGCGTTCGTCACTAACCTGATGAGCGGCATTTCGCATTATGGCAACGGACCCGCAGTGAT
CTATTACGGCGCTGGCTATGTCTCGCAACGAGAATGGTGGCGGCTGGGCTTTATCGTCATGTTGTTGAACATTGTCATCT
GGTTCGGACTGGGGGCGGTGTGGTGGAAAATTCTTGGCCTGTGGTAA

Upstream 100 bases:

>100_bases
CAGAGAATTTATTCGAACTAAAACCGATTTTCTGATTTGTCTGGCTGTACAGAAAACAGGATAAATTCCCTCCGCGAGCG
GTGGGAAACAGCTACAGGGA

Downstream 100 bases:

>100_bases
GTGGCCTGCGACAAACATCAATCAAGGAATAGTGTATGAACTTAAAAGATATTCAAGTGGCGATCGTCGGCAAACTAAGT
GATCGCTTAATGGCTAAAGT

Product: putative dicarboxylate transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MHMSCYLKVLICAIVALAIWFFPVPDGLTPLTWHILAIFITTVVAFILQPLPVGAIALIAISFIMLTGMMKTSEALKGFS
STTVWLIVAAFLYAKGFIKTGLGRRIAYLLIRGFGGSSLRLGYTLALSDMIIAPATPSNTARAGGILFPIVRSVSNSFGS
EPDQGPRKIGAYLMQTVFHSNCLSSSMFMTASAPNALIVSLAASTLFVDISWGMWTLSALVPGIIAFITMPLVIYKLYPP
EIKKTPEAKALAQEELIKMGPVTRDERVTIGIFLLSLLAWSTSKWTGLDATAVALSGVCLMLMTRIITWQDVQSEKGAWD
ILVWLGVMICMADKLNQLGLFKWFAVTTSALFTGIPWEITLTVLLIVYCYSHYFFAGSTPHVVAMYAAFGSVSVAAGAPP
MMAALSLAFVTNLMSGISHYGNGPAVIYYGAGYVSQREWWRLGFIVMLLNIVIWFGLGAVWWKILGLW

Sequences:

>Translated_468_residues
MHMSCYLKVLICAIVALAIWFFPVPDGLTPLTWHILAIFITTVVAFILQPLPVGAIALIAISFIMLTGMMKTSEALKGFS
STTVWLIVAAFLYAKGFIKTGLGRRIAYLLIRGFGGSSLRLGYTLALSDMIIAPATPSNTARAGGILFPIVRSVSNSFGS
EPDQGPRKIGAYLMQTVFHSNCLSSSMFMTASAPNALIVSLAASTLFVDISWGMWTLSALVPGIIAFITMPLVIYKLYPP
EIKKTPEAKALAQEELIKMGPVTRDERVTIGIFLLSLLAWSTSKWTGLDATAVALSGVCLMLMTRIITWQDVQSEKGAWD
ILVWLGVMICMADKLNQLGLFKWFAVTTSALFTGIPWEITLTVLLIVYCYSHYFFAGSTPHVVAMYAAFGSVSVAAGAPP
MMAALSLAFVTNLMSGISHYGNGPAVIYYGAGYVSQREWWRLGFIVMLLNIVIWFGLGAVWWKILGLW
>Mature_468_residues
MHMSCYLKVLICAIVALAIWFFPVPDGLTPLTWHILAIFITTVVAFILQPLPVGAIALIAISFIMLTGMMKTSEALKGFS
STTVWLIVAAFLYAKGFIKTGLGRRIAYLLIRGFGGSSLRLGYTLALSDMIIAPATPSNTARAGGILFPIVRSVSNSFGS
EPDQGPRKIGAYLMQTVFHSNCLSSSMFMTASAPNALIVSLAASTLFVDISWGMWTLSALVPGIIAFITMPLVIYKLYPP
EIKKTPEAKALAQEELIKMGPVTRDERVTIGIFLLSLLAWSTSKWTGLDATAVALSGVCLMLMTRIITWQDVQSEKGAWD
ILVWLGVMICMADKLNQLGLFKWFAVTTSALFTGIPWEITLTVLLIVYCYSHYFFAGSTPHVVAMYAAFGSVSVAAGAPP
MMAALSLAFVTNLMSGISHYGNGPAVIYYGAGYVSQREWWRLGFIVMLLNIVIWFGLGAVWWKILGLW

Specific function: Might be a malate transporter [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family [H]

Homologues:

Organism=Escherichia coli, GI1786986, Length=476, Percent_Identity=37.6050420168067, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1786829, Length=481, Percent_Identity=37.6299376299376, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1789444, Length=471, Percent_Identity=35.244161358811, Blast_Score=271, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 51011; Mature: 51011

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMSCYLKVLICAIVALAIWFFPVPDGLTPLTWHILAIFITTVVAFILQPLPVGAIALIA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ISFIMLTGMMKTSEALKGFSSTTVWLIVAAFLYAKGFIKTGLGRRIAYLLIRGFGGSSLR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LGYTLALSDMIIAPATPSNTARAGGILFPIVRSVSNSFGSEPDQGPRKIGAYLMQTVFHS
EEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
NCLSSSMFMTASAPNALIVSLAASTLFVDISWGMWTLSALVPGIIAFITMPLVIYKLYPP
HHHCCCEEEEECCCCHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EIKKTPEAKALAQEELIKMGPVTRDERVTIGIFLLSLLAWSTSKWTGLDATAVALSGVCL
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MLMTRIITWQDVQSEKGAWDILVWLGVMICMADKLNQLGLFKWFAVTTSALFTGIPWEIT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LTVLLIVYCYSHYFFAGSTPHVVAMYAAFGSVSVAAGAPPMMAALSLAFVTNLMSGISHY
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHC
GNGPAVIYYGAGYVSQREWWRLGFIVMLLNIVIWFGLGAVWWKILGLW
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHMSCYLKVLICAIVALAIWFFPVPDGLTPLTWHILAIFITTVVAFILQPLPVGAIALIA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ISFIMLTGMMKTSEALKGFSSTTVWLIVAAFLYAKGFIKTGLGRRIAYLLIRGFGGSSLR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LGYTLALSDMIIAPATPSNTARAGGILFPIVRSVSNSFGSEPDQGPRKIGAYLMQTVFHS
EEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
NCLSSSMFMTASAPNALIVSLAASTLFVDISWGMWTLSALVPGIIAFITMPLVIYKLYPP
HHHCCCEEEEECCCCHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EIKKTPEAKALAQEELIKMGPVTRDERVTIGIFLLSLLAWSTSKWTGLDATAVALSGVCL
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MLMTRIITWQDVQSEKGAWDILVWLGVMICMADKLNQLGLFKWFAVTTSALFTGIPWEIT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LTVLLIVYCYSHYFFAGSTPHVVAMYAAFGSVSVAAGAPPMMAALSLAFVTNLMSGISHY
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHC
GNGPAVIYYGAGYVSQREWWRLGFIVMLLNIVIWFGLGAVWWKILGLW
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 12949159 [H]