Click here to switch to the map view.

The map label for this gene is pps

Identifier: 218699730

GI number: 218699730

Start: 1416047

End: 1418425

Strand: Direct

Name: pps

Synonym: ECIAI39_1351

Alternate gene names: 218699730

Gene position: 1416047-1418425 (Clockwise)

Preceding gene: 218699726

Following gene: 218699731

Centisome position: 27.59

GC content: 54.77

Gene sequence:

>2379_bases
ATGTCCAACAATGGCTCGTCACCGCTGGTGCTTTGGTATAACCAACTCGGCATGAATGATGTAGACAGGGTTGGGGGCAA
AAATGCCTCCCTGGGTGAAATGATTACTAACCTTTCCGGAATGGGTGTTTCCGTTCCGAATGGTTTCGCCACAACCGCCG
ACGCGTTTAACCAGTTTCTGGACCAAAGCGGCGTAAACCAGCGCATTTATGAACTGCTGGATAAAACGGATATTGACGAT
GTTACCCAGCTTGCGAAAGCGGGCGCGCAAATCCGCCAGTGGATTATCGACACTCCCTTCCAGCCTGAGCTGGAAAACGC
CATCCGCGAAGCCTATGCACAGCTTTCCGCCGATGACGAAAACGCCTCGTTTGCGGTGCGTTCCTCCGCTACTGCAGAAG
ATATGCCGGACGCTTCTTTTGCCGGTCAGCAGGAAACCTTCCTCAACGTTCAGGGTTTTGACGCCGTTCTCGTGGCAGTG
AAACATGTATTTGCTTCTCTGTTTAACGATCGCGCCATCTCTTATCGTGTGCACCAGGGTTACGATCACCGTGGCGTGGC
GCTCTCCGCCGGTGTTCAGCGGATGGTGCGCTCCGACCTCGCATCTTCTGGCGTGATGTTCTCCATTGATACCGAATCCG
GCTTTGACCAGGTGGTGTTTATCACTTCCGCATGGGGCCTTGGGGAAATGGTCGTGCAGGGCGCGGTTAACCCGGATGAG
TTTTACGTGCATAAACCGACACTGGCGGCGAATCGCCCGGCTATCGTGCGCCGCACCATGGGGTCGAAAAAAATCCGCAT
GGTTTACGCGCCGACCCAGGAGCACGGCAAGCAGGTTAAAATCGAAGACGTACCGCAGGAACAGCGTGACATCTTCTCGC
TGACCAACGAAGAAGTGCAGGAACTGGCAAAACAGGCCGTACAAATTGAGAAACACTACGGTCGCCCGATGGATATTGAG
TGGGCGAAAGATGGCCACACCGGCAAACTGTTCATTGTGCAGGCGCGTCCGGAAACCGTGCGCTCACGCGGTCAGGTCAT
GGAGCGTTATACGCTGCATTCACAGGGTAAGATTATCGCCGAAGGCCGTGCTATCGGTCATCGCATCGGCGCGGGTCCGG
TGAAAGTCATCCATGACATCAGCGAAATGAACCGCATCGAACCTGGCGACGTGCTGGTTACTGACATGACCGACCCGGAC
TGGGAACCGATCATGAAGAAAGCATCTGCCATCGTCACCAACCGTGGCGGTCGTACCTGTCACGCGGCGATCATCGCTCG
TGAACTGGGTATTCCGGCGGTAGTGGGCTGTGGAGATGCAACAGAACGGATGAAAGACGGTGAGAACGTCACTGTTTCTT
GTGCCGAAGGTGATACCGGTTACGTCTATGCGGAGTTGCTGGAATTTAGCGTGAAAAGCTCTAGCGTAGAAACGATGCCG
GACCTGCCGTTGAAGGTGATGATGAACGTCGGTAACCCGGACCGAGCTTTCGACTTCGCCTGTCTGCCGAACGAAGGCGT
GGGACTTGCGCGTCTGGAATTTATCATCAACCGTATGATTGGCGTCCACCCACGCGCGCTGCTTGAGTTTGACGATCAAG
AACCGCAGTTGCAAAACGAAATCCGCGAGATGATGAAAGGCTTTGATTCTCCGCGTGAATTTTACGTTGGGCGTTTGACT
GAAGGGATCGCGACGCTGGGTGCCGCGTTTTATCCGAAGCGCGTCATTGTCCGTCTCTCTGATTTTAAATCGAACGAATA
TGCCAACCTGGTCGGTGGTGAGCGTTACGAGCCAGATGAAGAGAACCCGATGCTCGGCTTCCGTGGCGCGGGCCGCTATG
TTTCCGACAGCTTCCGCGACTGCTTCGCGCTGGAGTGTGAAGCAGTGAAACGTGTGCGCAACGACATGGGGCTGACCAAC
GTTGAGATCATGATCCCGTTCGTGCGTACCGTAGATCAGGCGAAAGCGGTGGTTGACGAACTGGCGCGTCAGGGGCTGAA
ACGTGGTGAGAACGGGCTGAAAATCATCATGATGTGTGAAATTCCGTCCAACGCCTTGCTGGCAGAGCAGTTCCTCGAAT
ATTTCGACGGCTTCTCAATTGGCTCAAACGACATGACACAGCTGGCGCTCGGTCTGGACCGCGACTCCGGCGTGGTGTCT
GAACTGTTCGATGAGCGCAACGACGCCGTGAAAGCATTGCTGTCGATGGCTATCCGTGCTGCGAAGAAACAGGGCAAATA
TGTTGGCATTTGCGGTCAGGGTCCATCTGACCACGAAGACTTTGCCGCCTGGTTGATGGAAGAGGGGATCGATAGCCTGT
CTCTGAACCCGGATACTGTGGTGCAAACCTGGTTAAGCCTGGCTGAACTTAACAAGTAA

Upstream 100 bases:

>100_bases
TTGAACGATTCACCGTTTTTTTCATCCGGTTAAATATGCAAAGATAAATGCGCAGAAATGTGTTTCTCAAACCGTTCATT
TATCACAAAAGGATTGTTCG

Downstream 100 bases:

>100_bases
ATAAAAACCCGGCGGCGTTCAGTCGCCGGGTCCATTTCTATATCCCCACCTTTTAACAACCTGCTTTGGGCAAACGCGCT
CATGCAATATAAAAAGTTTC

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 792; Mature: 791

Protein sequence:

>792_residues
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDD
VTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
FYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIE
WAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPD
WEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMP
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLT
EGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTN
VEIMIPFVRTVDQAKAVVDELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELNK

Sequences:

>Translated_792_residues
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDD
VTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
FYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIE
WAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPD
WEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMP
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLT
EGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTN
VEIMIPFVRTVDQAKAVVDELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELNK
>Mature_791_residues
SNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDV
TQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVK
HVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDEF
YVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEW
AKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDW
EPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMPD
LPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTE
GIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNV
EIMIPFVRTVDQAKAVVDELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSE
LFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELNK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=792, Percent_Identity=99.7474747474748, Blast_Score=1635, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=449, Percent_Identity=26.9487750556793, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788726, Length=192, Percent_Identity=34.8958333333333, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI48994992, Length=190, Percent_Identity=31.0526315789474, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789193, Length=408, Percent_Identity=25.9803921568627, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17564524, Length=331, Percent_Identity=25.9818731117825, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87408; Mature: 87277

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFL
CCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHH
DQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDE
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
NASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQG
CCEEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
CCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCC
FYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQ
EEEECCCCCCCCCHHHHHHCCCCEEEEEECCCHHCCCEEEECCCCHHHHHHHHHCHHHHH
ELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIA
HHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEE
EGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASAIVTNRGGRTC
CCHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHH
HAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMP
HHHHHHHHCCCCEEEECCCHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCC
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNE
CCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEECCCCCCHHHHH
IREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHCCCCCCCCCCC
ENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVDE
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
LARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHH
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTV
HHHHCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHH
VQTWLSLAELNK
HHHHHHHHHCCC
>Mature Secondary Structure 
SNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFL
CCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHH
DQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDE
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
NASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQG
CCEEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
CCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCC
FYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQ
EEEECCCCCCCCCHHHHHHCCCCEEEEEECCCHHCCCEEEECCCCHHHHHHHHHCHHHHH
ELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIA
HHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEE
EGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASAIVTNRGGRTC
CCHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHH
HAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMP
HHHHHHHHCCCCEEEECCCHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCC
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNE
CCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEECCCCCCHHHHH
IREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHCCCCCCCCCCC
ENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVDE
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
LARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHH
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTV
HHHHCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHH
VQTWLSLAELNK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]