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The map label for this gene is gyaR [H]

Identifier: 218699732

GI number: 218699732

Start: 1420350

End: 1421309

Strand: Direct

Name: gyaR [H]

Synonym: ECIAI39_1353

Alternate gene names: 218699732

Gene position: 1420350-1421309 (Clockwise)

Preceding gene: 218699731

Following gene: 218699733

Centisome position: 27.68

GC content: 49.9

Gene sequence:

>960_bases
ATGAACTTAAAAGATATTCAAGTGGCGATCGTCGGCAAACTAAGTGATCGCTTAATGGCTAAAGTTGAATCTACCTTCAC
CGCATATCGCCTGTGGGAACTGGCTGATGAAGGCGCTTTTTACACGCAAATCGCGCCGGATATTCAGGCGCTGGTGACCA
GTGGTAATCCGGTAATGGGGGCTTCTCGGGCGCTAATAGAAAAATTTCCGAATCTGAAGATTATTGCCAGCAACGGTGTG
GGTTATGACCCTATTGATATCGTGGCAGCCAGGGAGCATGGCGTTATCGTCACCAACACACCTGGCGTGCTTAATGACTG
TGTCGCAGACATTGGTATAGCGTTGTTACTCAATGTTGCCCGACGTATTAACATTGCCGATCGTTATGTTCGTGACGGCC
GTTGGCCAAACGAGGGGCGTTTCCCGATGGCGACGAAAGTGAGTGGCAAACGTTGCGGAATTGTCGGTCTGGGGAACATT
GGTAACGCAGTAGCCCGCCGTGCAGCAGCATTTGAAATGGAAATTCACTATTTCGACCCAAAACCGCATAACAAACCTGA
TTGGACGGCGCATGATTCGTTGGTTTCTCTGGCGCAAGCGGTGGACTTCCTCGTTCTGACGCTGCCAGGCGGCGCGAGTA
CGCGCGGCGTTATTGACAAAACGGTGCTGCAGGCTCTGGGGAAAACCGGGCATTTGATCAGTATTTCCCGGGGATCAGTG
GTGAACGAAACCGATCTGATTGATGCTCTGCAAAATAACATTATTGCTGGTGCGGCACTGGACGTTTATGCCAACGAACC
ACATGTGCCAGAACAATTAATGTCGCTGGATAACGTTGTCCTTACGCCACATATCGCCAGTGGTACGAGCGAAACGTTTA
ATGCGATGGCGGATTTGGTATTTGATAACTTACAGGCTTTCTTTAGCGGACGGCCAGTTATTACCCCTGTTACTCAATAA

Upstream 100 bases:

>100_bases
TGTTGAACATTGTCATCTGGTTCGGACTGGGGGCGGTGTGGTGGAAAATTCTTGGCCTGTGGTAAGTGGCCTGCGACAAA
CATCAATCAAGGAATAGTGT

Downstream 100 bases:

>100_bases
GGCGGAAAATATGATTTATGGTGATATCGCGTATCTTAGTGAACAACAAAAGCGGACATTTCATCCTGTCATCACTCGTG
TCATTGAGTATCTTTCACAA

Product: putative 2-hydroxyacid dehydrogenase/reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MNLKDIQVAIVGKLSDRLMAKVESTFTAYRLWELADEGAFYTQIAPDIQALVTSGNPVMGASRALIEKFPNLKIIASNGV
GYDPIDIVAAREHGVIVTNTPGVLNDCVADIGIALLLNVARRINIADRYVRDGRWPNEGRFPMATKVSGKRCGIVGLGNI
GNAVARRAAAFEMEIHYFDPKPHNKPDWTAHDSLVSLAQAVDFLVLTLPGGASTRGVIDKTVLQALGKTGHLISISRGSV
VNETDLIDALQNNIIAGAALDVYANEPHVPEQLMSLDNVVLTPHIASGTSETFNAMADLVFDNLQAFFSGRPVITPVTQ

Sequences:

>Translated_319_residues
MNLKDIQVAIVGKLSDRLMAKVESTFTAYRLWELADEGAFYTQIAPDIQALVTSGNPVMGASRALIEKFPNLKIIASNGV
GYDPIDIVAAREHGVIVTNTPGVLNDCVADIGIALLLNVARRINIADRYVRDGRWPNEGRFPMATKVSGKRCGIVGLGNI
GNAVARRAAAFEMEIHYFDPKPHNKPDWTAHDSLVSLAQAVDFLVLTLPGGASTRGVIDKTVLQALGKTGHLISISRGSV
VNETDLIDALQNNIIAGAALDVYANEPHVPEQLMSLDNVVLTPHIASGTSETFNAMADLVFDNLQAFFSGRPVITPVTQ
>Mature_319_residues
MNLKDIQVAIVGKLSDRLMAKVESTFTAYRLWELADEGAFYTQIAPDIQALVTSGNPVMGASRALIEKFPNLKIIASNGV
GYDPIDIVAAREHGVIVTNTPGVLNDCVADIGIALLLNVARRINIADRYVRDGRWPNEGRFPMATKVSGKRCGIVGLGNI
GNAVARRAAAFEMEIHYFDPKPHNKPDWTAHDSLVSLAQAVDFLVLTLPGGASTRGVIDKTVLQALGKTGHLISISRGSV
VNETDLIDALQNNIIAGAALDVYANEPHVPEQLMSLDNVVLTPHIASGTSETFNAMADLVFDNLQAFFSGRPVITPVTQ

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=250, Percent_Identity=35.6, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI23308577, Length=224, Percent_Identity=32.1428571428571, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI61743967, Length=265, Percent_Identity=30.188679245283, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI4557497, Length=265, Percent_Identity=30.188679245283, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI145580578, Length=265, Percent_Identity=29.0566037735849, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI4557499, Length=265, Percent_Identity=29.0566037735849, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI145580575, Length=265, Percent_Identity=29.0566037735849, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI87082289, Length=315, Percent_Identity=33.6507936507937, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787645, Length=270, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789279, Length=222, Percent_Identity=31.5315315315315, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081824, Length=141, Percent_Identity=29.7872340425532, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=243, Percent_Identity=30.4526748971193, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25147481, Length=233, Percent_Identity=31.7596566523605, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=252, Percent_Identity=27.3809523809524, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=241, Percent_Identity=29.4605809128631, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322116, Length=244, Percent_Identity=28.6885245901639, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321253, Length=318, Percent_Identity=26.4150943396226, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6325144, Length=310, Percent_Identity=25.1612903225806, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324964, Length=274, Percent_Identity=24.4525547445255, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45552429, Length=254, Percent_Identity=30.7086614173228, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24585514, Length=254, Percent_Identity=30.7086614173228, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI28574282, Length=254, Percent_Identity=30.7086614173228, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI45551003, Length=254, Percent_Identity=30.7086614173228, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI28574284, Length=254, Percent_Identity=30.7086614173228, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI28574286, Length=256, Percent_Identity=30.078125, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24585516, Length=252, Percent_Identity=29.3650793650794, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI28571528, Length=255, Percent_Identity=32.9411764705882, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19921140, Length=251, Percent_Identity=29.8804780876494, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24646446, Length=232, Percent_Identity=34.051724137931, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24646448, Length=232, Percent_Identity=34.051724137931, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24646452, Length=232, Percent_Identity=34.051724137931, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24646450, Length=232, Percent_Identity=34.051724137931, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI62472511, Length=232, Percent_Identity=34.051724137931, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 34293; Mature: 34293

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKDIQVAIVGKLSDRLMAKVESTFTAYRLWELADEGAFYTQIAPDIQALVTSGNPVMG
CCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCC
ASRALIEKFPNLKIIASNGVGYDPIDIVAAREHGVIVTNTPGVLNDCVADIGIALLLNVA
HHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHH
RRINIADRYVRDGRWPNEGRFPMATKVSGKRCGIVGLGNIGNAVARRAAAFEMEIHYFDP
HHCCHHHHHHHCCCCCCCCCCCEEEECCCCEECEEEECCCCHHHHHHHHEEEEEEEEECC
KPHNKPDWTAHDSLVSLAQAVDFLVLTLPGGASTRGVIDKTVLQALGKTGHLISISRGSV
CCCCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCC
VNETDLIDALQNNIIAGAALDVYANEPHVPEQLMSLDNVVLTPHIASGTSETFNAMADLV
CCHHHHHHHHHCCEEECEEEEECCCCCCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
FDNLQAFFSGRPVITPVTQ
HHHHHHHHCCCCCEECCCC
>Mature Secondary Structure
MNLKDIQVAIVGKLSDRLMAKVESTFTAYRLWELADEGAFYTQIAPDIQALVTSGNPVMG
CCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCC
ASRALIEKFPNLKIIASNGVGYDPIDIVAAREHGVIVTNTPGVLNDCVADIGIALLLNVA
HHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHH
RRINIADRYVRDGRWPNEGRFPMATKVSGKRCGIVGLGNIGNAVARRAAAFEMEIHYFDP
HHCCHHHHHHHCCCCCCCCCCCEEEECCCCEECEEEECCCCHHHHHHHHEEEEEEEEECC
KPHNKPDWTAHDSLVSLAQAVDFLVLTLPGGASTRGVIDKTVLQALGKTGHLISISRGSV
CCCCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCC
VNETDLIDALQNNIIAGAALDVYANEPHVPEQLMSLDNVVLTPHIASGTSETFNAMADLV
CCHHHHHHHHHCCEEECEEEEECCCCCCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
FDNLQAFFSGRPVITPVTQ
HHHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA