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The map label for this gene is yjjW [H]

Identifier: 218692767

GI number: 218692767

Start: 5181929

End: 5182792

Strand: Reverse

Name: yjjW [H]

Synonym: ECED1_5250

Alternate gene names: 218692767

Gene position: 5182792-5181929 (Counterclockwise)

Preceding gene: 218692768

Following gene: 218692758

Centisome position: 99.49

GC content: 56.13

Gene sequence:

>864_bases
ATGAACAGCAGATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTT
TTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGGCGCTGTAATGATTGCGGAGAGTGCG
TGCCGCAGTGTCCGCATCAGGCGTTGCAGATTGTCGACGGCAAAGTGGCGTGGAGCGCGGCTGCCTGCGAGCAATGTGAT
ACCTGCCTGAAGATGTGTCCGCAACATGCTACGCCAATGGCGCAATCCATGAGCGTGGACGAGGTGCTTAACCATGTCCG
CAAAGCGGTTCTGTTTATTGAAGGGATAACGGTAAGCGGCGGCGAAGCCACGACCCAGTTGCCGTTTGTGGTGGCGCTGT
TTACTGCCATCAAAAACGATCCGCAACTGTGCCATCTCACCTGCCTCGTGGACAGTAACGGCATGTTGAGCGAAACCGGC
TGGGAAAAATTGCTCCCGGTGTGTGACGGCGCAATGCTCGATCTCAAAGCGTGGGGGAGCGAATGTCATCAGCAATTAAC
CGGACGCGATAATCAGCAGATCAAGCGCAGCATCTGTTTGCTGGCAGAGCGCGGTAAGCTGGCGGAACTGCGTTTGCTGG
TAATCCCTGGCCAGGTGGATTATTTGCAACATATCGAAGAACTGGCGGCGTTAATCAAGGGACTTGGCGATGTTCCGGTA
CGCCTGAACGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGCCGAGCGCCACGCCGGAGGATGTGGAACA
ACTGGCTGATGCGTTACGGGAGCGCGGGGTGAGCCGGTTAATATTTCCGGCGCTATATTTGTGA

Upstream 100 bases:

>100_bases
AAATATCGCGCCGAAGGTTCACGCACCAACACTACCTGGCTGGGAGAAGAAGCCGCACGCAACACTCGTATTCTGGAACG
CCAGCCGCGCGTGATAAGCC

Downstream 100 bases:

>100_bases
CATGCCGGATGTGGCCTACGGATCGGCACAGACCTGTAGGCATGATAAGACGCGTCAAGCGTCGCATCAGGCATTGTGCG
CCAGCTGCCGGATGCGGCGT

Product: putative pyruvate formate lyase activating enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQALQIVDGKVAWSAAACEQCD
TCLKMCPQHATPMAQSMSVDEVLNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAALIKGLGDVPV
RLNAFHAHGVYGEAQSWPSATPEDVEQLADALRERGVSRLIFPALYL

Sequences:

>Translated_287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQALQIVDGKVAWSAAACEQCD
TCLKMCPQHATPMAQSMSVDEVLNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAALIKGLGDVPV
RLNAFHAHGVYGEAQSWPSATPEDVEQLADALRERGVSRLIFPALYL
>Mature_287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQALQIVDGKVAWSAAACEQCD
TCLKMCPQHATPMAQSMSVDEVLNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAALIKGLGDVPV
RLNAFHAHGVYGEAQSWPSATPEDVEQLADALRERGVSRLIFPALYL

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790839, Length=287, Percent_Identity=95.4703832752613, Blast_Score=563, Evalue=1e-162,
Organism=Escherichia coli, GI1787130, Length=289, Percent_Identity=27.3356401384083, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790389, Length=254, Percent_Identity=23.2283464566929, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 31350; Mature: 31350

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS01087 RADICAL_ACTIVATING ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
6.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQ
CCCHHHHHHHHCCCEECCCCCHHEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHCHHH
ALQIVDGKVAWSAAACEQCDTCLKMCPQHATPMAQSMSVDEVLNHVRKAVLFIEGITVSG
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEECC
GEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETGWEKLLPVCDGAMLDLKAWGS
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
ECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAALIKGLGDVPV
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCE
RLNAFHAHGVYGEAQSWPSATPEDVEQLADALRERGVSRLIFPALYL
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQ
CCCHHHHHHHHCCCEECCCCCHHEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHCHHH
ALQIVDGKVAWSAAACEQCDTCLKMCPQHATPMAQSMSVDEVLNHVRKAVLFIEGITVSG
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEECC
GEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETGWEKLLPVCDGAMLDLKAWGS
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
ECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAALIKGLGDVPV
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCE
RLNAFHAHGVYGEAQSWPSATPEDVEQLADALRERGVSRLIFPALYL
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]