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The map label for this gene is uhpB [H]

Identifier: 218692577

GI number: 218692577

Start: 4976192

End: 4977733

Strand: Reverse

Name: uhpB [H]

Synonym: ECED1_5044

Alternate gene names: 218692577

Gene position: 4977733-4976192 (Counterclockwise)

Preceding gene: 218692578

Following gene: 218692576

Centisome position: 95.55

GC content: 52.01

Gene sequence:

>1542_bases
ATGTCGCGTAACCTTCGTCATTGGGTGATTTCGCTGTTTATTGTGCTGGCCTGGGGAAGCGGCTGGCTGATGTTGTGGAC
GCTCAGCTTTTATCTGACCAATAACGGTCAACAGGCAGTGTTATTTCTGCCGCACGGAGTTTACCTCGCTTTTCTTATTT
TGCACTCTCGCCGTTATTGGCCTGCGTTGGTGCTCCCCCCTTTGCTGATGATGTTCTGGCTGCACAGCGAGCAACTGTTG
AATGGCTATCTCATGCTGGCAACGCCGGTTATTAGCCTGCTTCCTGCCATCATTGCACAAAACGTCTGGCACCGTTTTCC
ACTTTACTGGCAGCGTCTGACGTTGCTGTTAGCCTCAGTAACAGCAGCTTCATTGCTGAATACAGCCATGCTAACCCCTT
TTATGTCAGGCCCAATAATGCTGCCCGGTCTGACCTCATTTACCGGCGGCGTATTGTTGACGCCGTTTGTCTATCTTATC
TTTGAGTTTCTGCGCCAGCAGCATCGCTATCAGCTTCTGAGACTCGATACGCATAATCCCCCCTTGCGAACCTCTTTAAT
CATCTGGTGTAGCCTGTTTTTTATTATTGGTATCGGCACCCAGATGGTGTTGTCGCCTGAAATCGAACGCCTGCTGCTGA
TCGTGGTATTTCTACCCAATGTAGTCATGGCCTGGAAATTTGGCTGGCAAGGGGGCGTACTCTCGGGGCTTTTGGGCAGC
ATGATGATCACTATTGCCCGCCAGATTGGTGTGGGCTTTAGTAACCTGGTGGAACTGGAGATTTTTCTCGCAACCCAGGC
ATTGCTGGGAATTGGCCTGGGCATTGCCATTAGTCGGCAGCAACATCTGGCGCTAAACCTGCATCATTACCGTCAGCGAC
TGGAGGCAGAACTGGCAGCACGGCGGGCATTGGCTGAAAAGCTGATCCATACCGAGGAAGACACGCGTAAAAGCCTGGCT
CGCGAGCTGCACGACGAAATCGGTCAGAACATTACCGCCATTCAGATCCAGTCGCAACTGGTGACGCGGGCACGCGATCC
GGCGCAAGTTCATTCCGCAGCCAGCCAAATCAACGAACTCGCCCGCCGCATTCATCTCTCCACCCGTCAGTTGCTGCGCC
AGTTGCGGCCCCCCGCGCTTGATGAACTGACATTCCGCGAAGCATTACTTCATCTGATTAATGAATTTGCCTTTAGTGAA
CTCGGCATTCATTGCCAGTTTACCTATCAACTAACCACCACGCCAGAAAACGAAACGGTTCGCTTTACACTGTATCGGTT
ATTGCAGGAGTTACTCAATAATGTCTGTAAACACGCCGGAGCCAGCGAAGTGACGATTATTCTGCGCCAGCAGGGAGAGA
TATTGCACCTAGAGGTCAGCGACAACGGTGTGGGGATTGCCAGCGGGAAAATGACCGGATTTGGCATTCAGGGGATGCGT
GAACGCGTCAGTGCCCTGGGCGGCGACTTAACGCTCGAAAAACATAACGGTACGCGGGTAATTGTTAACCTGCCCACATT
TTTGCAACAAATCGCCGGATAA

Upstream 100 bases:

>100_bases
TACATGTACATCGCGCCAACGTGCTGGGAAAACTGAACTGTCATAGCACCATTGAGCTGGTGCATTTTGCACTCAACCAC
CATCTGCTGGCGGGGCATTA

Downstream 100 bases:

>100_bases
CCAGGAAAAAGTCTTAGTCATCCCGGATTTTCTCTCATCCTCTTTCACTTTCATTTTCATATAGTCAGCAACATGGAGAC
GACCATGCACGCGCGTTCAG

Product: putative sensor histidine protein kinase (uhpB-like)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSRNLRHWVISLFIVLAWGSGWLMLWTLSFYLTNNGQQAVLFLPHGVYLAFLILHSRRYWPALVLPPLLMMFWLHSEQLL
NGYLMLATPVISLLPAIIAQNVWHRFPLYWQRLTLLLASVTAASLLNTAMLTPFMSGPIMLPGLTSFTGGVLLTPFVYLI
FEFLRQQHRYQLLRLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFLPNVVMAWKFGWQGGVLSGLLGS
MMITIARQIGVGFSNLVELEIFLATQALLGIGLGIAISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKSLA
RELHDEIGQNITAIQIQSQLVTRARDPAQVHSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHLINEFAFSE
LGIHCQFTYQLTTTPENETVRFTLYRLLQELLNNVCKHAGASEVTIILRQQGEILHLEVSDNGVGIASGKMTGFGIQGMR
ERVSALGGDLTLEKHNGTRVIVNLPTFLQQIAG

Sequences:

>Translated_513_residues
MSRNLRHWVISLFIVLAWGSGWLMLWTLSFYLTNNGQQAVLFLPHGVYLAFLILHSRRYWPALVLPPLLMMFWLHSEQLL
NGYLMLATPVISLLPAIIAQNVWHRFPLYWQRLTLLLASVTAASLLNTAMLTPFMSGPIMLPGLTSFTGGVLLTPFVYLI
FEFLRQQHRYQLLRLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFLPNVVMAWKFGWQGGVLSGLLGS
MMITIARQIGVGFSNLVELEIFLATQALLGIGLGIAISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKSLA
RELHDEIGQNITAIQIQSQLVTRARDPAQVHSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHLINEFAFSE
LGIHCQFTYQLTTTPENETVRFTLYRLLQELLNNVCKHAGASEVTIILRQQGEILHLEVSDNGVGIASGKMTGFGIQGMR
ERVSALGGDLTLEKHNGTRVIVNLPTFLQQIAG
>Mature_512_residues
SRNLRHWVISLFIVLAWGSGWLMLWTLSFYLTNNGQQAVLFLPHGVYLAFLILHSRRYWPALVLPPLLMMFWLHSEQLLN
GYLMLATPVISLLPAIIAQNVWHRFPLYWQRLTLLLASVTAASLLNTAMLTPFMSGPIMLPGLTSFTGGVLLTPFVYLIF
EFLRQQHRYQLLRLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFLPNVVMAWKFGWQGGVLSGLLGSM
MITIARQIGVGFSNLVELEIFLATQALLGIGLGIAISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKSLAR
ELHDEIGQNITAIQIQSQLVTRARDPAQVHSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHLINEFAFSEL
GIHCQFTYQLTTTPENETVRFTLYRLLQELLNNVCKHAGASEVTIILRQQGEILHLEVSDNGVGIASGKMTGFGIQGMRE
RVSALGGDLTLEKHNGTRVIVNLPTFLQQIAG

Specific function: Member of the two-component regulatory system uhpB/uhpA involved in the regulation of the uptake of hexose phosphates. UhpB functions as a membrane-associated protein kinase that phosphorylates uhpA in response to environmental signals [H]

COG id: COG3851

COG function: function code T; Signal transduction histidine kinase, glucose-6-phosphate specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=509, Percent_Identity=33.2023575638507, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1787474, Length=228, Percent_Identity=29.8245614035088, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788812, Length=204, Percent_Identity=28.921568627451, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR007895
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3; PF05231 MASE1 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57938; Mature: 57807

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNLRHWVISLFIVLAWGSGWLMLWTLSFYLTNNGQQAVLFLPHGVYLAFLILHSRRYW
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCEEEEEECHHHHHHHHHHHCCCCC
PALVLPPLLMMFWLHSEQLLNGYLMLATPVISLLPAIIAQNVWHRFPLYWQRLTLLLASV
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAASLLNTAMLTPFMSGPIMLPGLTSFTGGVLLTPFVYLIFEFLRQQHRYQLLRLDTHNP
HHHHHHHHHHHHHHCCCCEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCC
PLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFLPNVVMAWKFGWQGGVLSGLLGS
CHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MMITIARQIGVGFSNLVELEIFLATQALLGIGLGIAISRQQHLALNLHHYRQRLEAELAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHH
RRALAEKLIHTEEDTRKSLARELHDEIGQNITAIQIQSQLVTRARDPAQVHSAASQINEL
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCHHHHHHHHHHHHHH
ARRIHLSTRQLLRQLRPPALDELTFREALLHLINEFAFSELGIHCQFTYQLTTTPENETV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCHH
RFTLYRLLQELLNNVCKHAGASEVTIILRQQGEILHLEVSDNGVGIASGKMTGFGIQGMR
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCEEECCCCCCCCHHHHH
ERVSALGGDLTLEKHNGTRVIVNLPTFLQQIAG
HHHHHCCCCEEEEECCCCEEEEECHHHHHHHCC
>Mature Secondary Structure 
SRNLRHWVISLFIVLAWGSGWLMLWTLSFYLTNNGQQAVLFLPHGVYLAFLILHSRRYW
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCEEEEEECHHHHHHHHHHHCCCCC
PALVLPPLLMMFWLHSEQLLNGYLMLATPVISLLPAIIAQNVWHRFPLYWQRLTLLLASV
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAASLLNTAMLTPFMSGPIMLPGLTSFTGGVLLTPFVYLIFEFLRQQHRYQLLRLDTHNP
HHHHHHHHHHHHHHCCCCEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCC
PLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFLPNVVMAWKFGWQGGVLSGLLGS
CHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MMITIARQIGVGFSNLVELEIFLATQALLGIGLGIAISRQQHLALNLHHYRQRLEAELAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHH
RRALAEKLIHTEEDTRKSLARELHDEIGQNITAIQIQSQLVTRARDPAQVHSAASQINEL
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCHHHHHHHHHHHHHH
ARRIHLSTRQLLRQLRPPALDELTFREALLHLINEFAFSELGIHCQFTYQLTTTPENETV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCHH
RFTLYRLLQELLNNVCKHAGASEVTIILRQQGEILHLEVSDNGVGIASGKMTGFGIQGMR
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCEEECCCCCCCCHHHHH
ERVSALGGDLTLEKHNGTRVIVNLPTFLQQIAG
HHHHHCCCCEEEEECCCCEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3301805; 1569007; 7686882; 9278503 [H]