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The map label for this gene is yfaW [H]

Identifier: 218690410

GI number: 218690410

Start: 2650342

End: 2651559

Strand: Reverse

Name: yfaW [H]

Synonym: ECED1_2713

Alternate gene names: 218690410

Gene position: 2651559-2650342 (Counterclockwise)

Preceding gene: 218690411

Following gene: 218690409

Centisome position: 50.9

GC content: 51.97

Gene sequence:

>1218_bases
ATGGAGAACATCATGACCCTACCAAAAATCAAACAGGTTCGCGCCTGGTTTACTGGCGGTGCGACAGCAGAAAAAGGCGC
TGGCGGCGGTGATTATCACGACCAGGGGGCGAATCACTGGATTGATGATCATATTGCCACCCCGATGAGTAAATACCGCG
AATACGAGCAGTCCCGCCAGTCATTTGGCATTAACGTTCTTGGCACGTTGATTGTTGAAGTTGAAGCAGAAAACGGCCAG
ACCGGATTCGCCGTTTCGACAGCCGGTGAAATGGGTTGTTTTATTGTCGAAAAACATCTTAACCGTTTCATTGAGGGTAA
ATGCGTCAGTGATATCAAACTGATCCACGATCAAATGCTCAATGCCACCCTGTATTACTCCGGCTCTGGTGGCCTGGTGA
TGAATACGATTTCCTGTGTCGATCTGGCTCTGTGGGATCTGTTCGGCAAAGTGGTCGGGCTTCCGGTTTATAAACTTTTA
GGCGGCGCAGTTCGTGATGAGATTCAGTTCTACGCCACAGGTGCGCGTCCGGATCTGGCAAAAGAGATGGGCTTTATCGG
TGGCAAAATGCCGACGCACTGGGGGCCACATGATGGTGATGCGGGGATCCGCAAAGATGCCGCTATGGTCGCGGATATGC
GTGAAAAATGCGGTGAGGATTTCTGGTTAATGCTCGACTGCTGGATGAGTCAGGACGTGAACTATGCCATCAAACTGGCC
CACGCTTGCGCGCCCTATAACCTGAAATGGATCGAAGAGTGCCTGCCGCCACAGCAGTATGAAGGTTATCGCGAACTGAA
ACACAACGCACCAGCGGGGATGATGGTCACCAGCGGTGAGCACCACGGCACACTGCAATCTTTCCGTACGCTTTCAGAAA
CCGGTATCGACATTATGCAGCCGGATGTTGGCTGGTGCGGTGGCTTAACCACGCTGGTGGAAATTGCCGCAATCGCCAAA
TCCAGGGGGCAACTGGTGGTGCCGCACGGTTCGTCTGTTTACTCCCACCATGCGGTGATCACCTTCACCAATACGCCATT
CAGTGAATTCCTGATGACCAGCCCGGATTGTTCAACGATGCGTCCGCAATTTGATCCGATTCTGCTTAATGAGCCGGTTC
CGGTGAATGGTCGTATTCATAAATCAGTGCTTGATAAACCCGGTTTCGGCGTCGAACTCAATCGTGACTGCAATTTGAAC
CCCCCCTACAGCCACTAA

Upstream 100 bases:

>100_bases
AATACCGTGATTATCTGGCGGGCAAGGCCATTGCCTGCGCCAGGGATATTTCACGTTTGTTGGGATGGAAAAGTCCCTTT
GACTTACAGGCCTCATAACA

Downstream 100 bases:

>100_bases
TCACTCCGGGCGTTGCTGTGACAACGGTAACGCCCACATATTCTAAGGAGCTTTTTATGAGCACCGCTTTGCTTGACGCC
GTGGTGAAGAAAAACCGCGC

Product: putative epimerase/enolase/dehydratase

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MENIMTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYREYEQSRQSFGINVLGTLIVEVEAENGQ
TGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLL
GGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYAIKLA
HACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAK
SRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLN
PPYSH

Sequences:

>Translated_405_residues
MENIMTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYREYEQSRQSFGINVLGTLIVEVEAENGQ
TGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLL
GGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYAIKLA
HACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAK
SRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLN
PPYSH
>Mature_405_residues
MENIMTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYREYEQSRQSFGINVLGTLIVEVEAENGQ
TGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLL
GGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYAIKLA
HACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAK
SRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLN
PPYSH

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510960, Length=401, Percent_Identity=97.7556109725686, Blast_Score=825, Evalue=0.0,
Organism=Escherichia coli, GI48994953, Length=243, Percent_Identity=30.8641975308642, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 44631; Mature: 44631

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIMTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYREYEQSRQ
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
SFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQML
HCCCEEEEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
NATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLA
CEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHCEEEEECCCCCHHH
KEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYAIKLA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCEEEEE
HACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGEHHGTLQSFRTLSETGIDIMQ
HHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEC
PDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTM
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEECCEEEEEECCCCHHHHHCCCCCCCCC
RPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLNPPYSH
CCCCCCEEECCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MENIMTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYREYEQSRQ
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
SFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQML
HCCCEEEEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
NATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLA
CEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHCEEEEECCCCCHHH
KEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYAIKLA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCEEEEE
HACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGEHHGTLQSFRTLSETGIDIMQ
HHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEC
PDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTM
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEECCEEEEEECCCCHHHHHCCCCCCCCC
RPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLNPPYSH
CCCCCCEEECCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA