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The map label for this gene is sucB [H]

Identifier: 218551368

GI number: 218551368

Start: 4250268

End: 4251422

Strand: Direct

Name: sucB [H]

Synonym: EFER_4149

Alternate gene names: 218551368

Gene position: 4250268-4251422 (Clockwise)

Preceding gene: 218551367

Following gene: 218551369

Centisome position: 92.62

GC content: 53.42

Gene sequence:

>1155_bases
ATGATCGAAATTACTGTCCCTGTATTACCGGAATCTGTCACGGAAGGCACGCTGACAACCTGGTGTAAACAAGAAGGCGA
GCACGTAAAGCGTGATGATGTGATTGCCGAGCTGGAAACCGATAAAGTCATTCTGGAAATCCCGGCTCCGCACGATGGCG
TGTTAAGCAATATTATCGTCAGCGAAGGTAGCACGGTCACGTCAGCGCAACTGTTGGCGCATCTTAAGCCGCAAGCTGCA
ATAGAAGAAACTGTCACACCAGTCACCGAAATCCTGGCGATGCCTTCCGCGCGGCTGGAGGCACAGCGTAGTGGTGTCGA
ACTTACTGATGTTGCGGGGAGCGGGAGAAATGGACGTATCCTGAAAGAGGATGTGCAGCGCGTAACTCCTGCACCAGCCA
CTCAGCCTGAACGGGTGGTGGAGATCGCGCCAGCTACACCTGTGACACCGGGTGCTCGTCAGGAACGTCGTGAGCCGATG
TCGCGCTTGCGCCAGCGAATTGCTGAGCGTCTGCTGGCTTCCCAACAAAACAACGCCATTCTCACCACGTTCAACGAAGT
AAATATGCAGAGCGTGATGGATTTACGCGCCCGCTGGAAAGATCGTTTTGCCGGGAAGCACGGCGTGAAGCTGGGCTTTA
TGTCCTTCTTTGTTAAGGCCGTTACCCGGGCGCTGGAACGCTTCCCCATTGTTAATGCCAGCGTCGATGGCAACGATATT
ATCTTGCGCGATTACTGTGATATTGGCATTGCGGTTAGCAGCAACCGTGGTCTGGTGGTGCCCGTATTACGCAATGCGCA
ATCACTCTCTTTGGTGGAAATTGAACGGCAAATTGCCGAATACGCCACACTGGCACGCAATGGCAAATTGCCGCTGGAGG
CGTTACAAGGTGGTACGTTCTCTATCACCAACGGCGGAACTTTTGGTTCGATGATGTCAACGCCAATCATTAACCCGCCG
CAGTCAGCCATTCTCGGTATGCATGCCATCACGCCGCGTCCGGTGGCAGAAAATGGTCAGGTTGTTATTCGTCCGATGAT
GTACCTTGCTCTCAGCTATGACCATCGCATTATTGATGGTCAGGAAGCCGTGCAGACGCTGGTGGCAATACGCGAGCTAC
TGGAGTCACCGGAACAACTGTTGCTGGATCTTTAA

Upstream 100 bases:

>100_bases
ATGGGCGTGTACATAAACAACAAATCGACGAATTTCTTGCTGCTGCGTTTGCAGATATTCAGCCATGAAAGGCGCACAAA
AATAATAAGGAGAACATTTT

Downstream 100 bases:

>100_bases
GGAAAGCGCCAATGATTACAATTTTCGATGTGGCAGTTATAGGTGGTGGGCCAGGTGGTTACGTAGCGGCACTGCGGGCT
GCGCAGAATGGGCTTTCCGT

Product: dihydrolipoamide succinyltransferase (E2 component)

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVILEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAA
IEETVTPVTEILAMPSARLEAQRSGVELTDVAGSGRNGRILKEDVQRVTPAPATQPERVVEIAPATPVTPGARQERREPM
SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAGKHGVKLGFMSFFVKAVTRALERFPIVNASVDGNDI
ILRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIERQIAEYATLARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPP
QSAILGMHAITPRPVAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL

Sequences:

>Translated_384_residues
MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVILEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAA
IEETVTPVTEILAMPSARLEAQRSGVELTDVAGSGRNGRILKEDVQRVTPAPATQPERVVEIAPATPVTPGARQERREPM
SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAGKHGVKLGFMSFFVKAVTRALERFPIVNASVDGNDI
ILRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIERQIAEYATLARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPP
QSAILGMHAITPRPVAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL
>Mature_384_residues
MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVILEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAA
IEETVTPVTEILAMPSARLEAQRSGVELTDVAGSGRNGRILKEDVQRVTPAPATQPERVVEIAPATPVTPGARQERREPM
SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAGKHGVKLGFMSFFVKAVTRALERFPIVNASVDGNDI
ILRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIERQIAEYATLARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPP
QSAILGMHAITPRPVAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=389, Percent_Identity=43.1876606683805, Blast_Score=301, Evalue=5e-82,
Organism=Homo sapiens, GI110671329, Length=422, Percent_Identity=28.9099526066351, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=29.5612009237875, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=26.3858093126386, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=26.3858093126386, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.1614906832298, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786946, Length=402, Percent_Identity=50.4975124378109, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1786305, Length=402, Percent_Identity=28.6069651741293, Blast_Score=151, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI25146366, Length=403, Percent_Identity=41.439205955335, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=28.5388127853881, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=25.4716981132075, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17538894, Length=336, Percent_Identity=29.7619047619048, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=390, Percent_Identity=44.3589743589744, Blast_Score=324, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=29.6213808463252, Blast_Score=153, Evalue=4e-38,
Organism=Drosophila melanogaster, GI18859875, Length=417, Percent_Identity=27.0983213429257, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24582497, Length=230, Percent_Identity=31.7391304347826, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI20129315, Length=230, Percent_Identity=31.7391304347826, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 41980; Mature: 41980

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVILEIPAPHDGVLSNIIV
CEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHEEECCCEEEEECCCCCCHHHHHHHH
SEGSTVTSAQLLAHLKPQAAIEETVTPVTEILAMPSARLEAQRSGVELTDVAGSGRNGRI
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCCC
LKEDVQRVTPAPATQPERVVEIAPATPVTPGARQERREPMSRLRQRIAERLLASQQNNAI
HHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LTTFNEVNMQSVMDLRARWKDRFAGKHGVKLGFMSFFVKAVTRALERFPIVNASVDGNDI
EEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCEE
ILRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIERQIAEYATLARNGKLPLEALQGGTF
EEEECCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEE
SITNGGTFGSMMSTPIINPPQSAILGMHAITPRPVAENGQVVIRPMMYLALSYDHRIIDG
EEECCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHCCCCEECH
QEAVQTLVAIRELLESPEQLLLDL
HHHHHHHHHHHHHHHCHHHHHHCC
>Mature Secondary Structure
MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVILEIPAPHDGVLSNIIV
CEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHEEECCCEEEEECCCCCCHHHHHHHH
SEGSTVTSAQLLAHLKPQAAIEETVTPVTEILAMPSARLEAQRSGVELTDVAGSGRNGRI
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCCC
LKEDVQRVTPAPATQPERVVEIAPATPVTPGARQERREPMSRLRQRIAERLLASQQNNAI
HHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LTTFNEVNMQSVMDLRARWKDRFAGKHGVKLGFMSFFVKAVTRALERFPIVNASVDGNDI
EEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCEE
ILRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIERQIAEYATLARNGKLPLEALQGGTF
EEEECCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEE
SITNGGTFGSMMSTPIINPPQSAILGMHAITPRPVAENGQVVIRPMMYLALSYDHRIIDG
EEECCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHCCCCEECH
QEAVQTLVAIRELLESPEQLLLDL
HHHHHHHHHHHHHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]