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The map label for this gene is odhL [H]

Identifier: 218551369

GI number: 218551369

Start: 4251434

End: 4252852

Strand: Direct

Name: odhL [H]

Synonym: EFER_4150

Alternate gene names: 218551369

Gene position: 4251434-4252852 (Clockwise)

Preceding gene: 218551368

Following gene: 218551370

Centisome position: 92.65

GC content: 52.78

Gene sequence:

>1419_bases
ATGATTACAATTTTCGATGTGGCAGTTATAGGTGGTGGGCCAGGTGGTTACGTAGCGGCACTGCGGGCTGCGCAGAATGG
GCTTTCCGTAGTATGTATTGATGATGGTGTGAATGCTCAGGGCGAACCTTCGCCTGGCGGTACGTGCCTCAATGTCGGCT
GCATTCCGTCAAAATCGTTACTGCAATCTTCTGAGCTGTATGCGCAAGTACAGCATGAGGCAAGCATTCATGGCGTTAAC
GTTGAAGGCGTATCTTTCAATGCTGCCGCGATGATTCAACGCAAAGACGCCATAGTAAGCCGCCTGACAATGGGCATCAG
CTTGCTGTTTAAGAAAAATAAAGTGAAGCACCTGTGCGGCCTCGCAACGTTAGAATGTGCTCAGGATGAGATTTGGCAAT
TACGCGTAAACGATCAACACCTTCACGCCCGTAATGTAGTGATTGCAACAGGTTCTCAGCCGCGTCAGCTGCCTGGCGTT
ACCATTGATAATCAACAGATCCTCGACAATCGTGGCGCCCTGGCATTGAGCGAAGTGCCACCGCGTCTCGGTGTGATCGG
TGCTGGCGTGATTGGCCTTGAACTGGGGTCTGTGTGGAATCGGGTCGGTTCAGACGTCACGTTGCTGGAGATGGCGCCAA
TGTTCTTGCCTGCACTGGAAGCTCGCCTGTCGAACGAAGTCCGTAAAGCAATGATTGCCAGTGGGATGAAAATGCAACTG
GCAGTAGAGATTGAGGCTATTGAACAACGGGATGACGGTGTGCATGTGCGCTGGCGGCAGGGTGAGAAGCAAGAAGAAAG
CCACTTCGATAAATTAATTCTTGCCATTGGGCGTGTACCGCGACTTTCTGGTGTCGATCTCGTGCAGCTCGGTCTGGAAG
CCGACAACCGTGGCGGTATTGCCGTCGATAACTTATGCCGTACCGGGAAAGCCGGGCTGTGGGCGATTGGTGATGTCGTA
CGAGGGCCGATGCTGGCGCATAAAGCGATGGCCGAAGGTGTGGTGGTGGCTGATCAAATTGCCGGACTGGCGGTAGAGCC
GGTTAATTTCGTGCTGATCCCTTCGGTGATTTACACCCAACCGGAAGTCGCGTGGGTAGGGGAAAATGAGGCCTCACTGA
AAGCCGCTGGCAGAGCCTTTAACAAAGGAAATTCTCTGTTTGCCGGTAACGGTCGCGCCTTAGCATTAGGTCAGGAAGGT
GGGCGTTGTACGTTATACAGCGATAAACACTCTGACCGCGTACTTGGCGGAGCGATTGTTGGCCCGCAGGCATCGGAGCT
GATTAATGAAATTGCGCTGGCGATGACTTTTAGCGCCTCTGGCGAGGATATTGCCTGTGCGGTACATGCGCATCCGACGT
TAAGCGAAGTCATCCACGAAGCAGCAATGGCCTTGAATAATAAAGTATTACACGGATAA

Upstream 100 bases:

>100_bases
GCATTATTGATGGTCAGGAAGCCGTGCAGACGCTGGTGGCAATACGCGAGCTACTGGAGTCACCGGAACAACTGTTGCTG
GATCTTTAAGGAAAGCGCCA

Downstream 100 bases:

>100_bases
CACACACCACTGAGGGAATATATGAATTTACATGAGTATCAGGCCAAATCGTTACTGGCTGGCATGGGGATGCCGTGTCC
AAAAGAGATAGCTATCCAAC

Product: dihydrolipoyl dehydrogenase, E3 component (lpd-like)

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MITIFDVAVIGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPSPGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVN
VEGVSFNAAAMIQRKDAIVSRLTMGISLLFKKNKVKHLCGLATLECAQDEIWQLRVNDQHLHARNVVIATGSQPRQLPGV
TIDNQQILDNRGALALSEVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPMFLPALEARLSNEVRKAMIASGMKMQL
AVEIEAIEQRDDGVHVRWRQGEKQEESHFDKLILAIGRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVV
RGPMLAHKAMAEGVVVADQIAGLAVEPVNFVLIPSVIYTQPEVAWVGENEASLKAAGRAFNKGNSLFAGNGRALALGQEG
GRCTLYSDKHSDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAVHAHPTLSEVIHEAAMALNNKVLHG

Sequences:

>Translated_472_residues
MITIFDVAVIGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPSPGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVN
VEGVSFNAAAMIQRKDAIVSRLTMGISLLFKKNKVKHLCGLATLECAQDEIWQLRVNDQHLHARNVVIATGSQPRQLPGV
TIDNQQILDNRGALALSEVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPMFLPALEARLSNEVRKAMIASGMKMQL
AVEIEAIEQRDDGVHVRWRQGEKQEESHFDKLILAIGRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVV
RGPMLAHKAMAEGVVVADQIAGLAVEPVNFVLIPSVIYTQPEVAWVGENEASLKAAGRAFNKGNSLFAGNGRALALGQEG
GRCTLYSDKHSDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAVHAHPTLSEVIHEAAMALNNKVLHG
>Mature_472_residues
MITIFDVAVIGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPSPGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVN
VEGVSFNAAAMIQRKDAIVSRLTMGISLLFKKNKVKHLCGLATLECAQDEIWQLRVNDQHLHARNVVIATGSQPRQLPGV
TIDNQQILDNRGALALSEVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPMFLPALEARLSNEVRKAMIASGMKMQL
AVEIEAIEQRDDGVHVRWRQGEKQEESHFDKLILAIGRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVV
RGPMLAHKAMAEGVVVADQIAGLAVEPVNFVLIPSVIYTQPEVAWVGENEASLKAAGRAFNKGNSLFAGNGRALALGQEG
GRCTLYSDKHSDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAVHAHPTLSEVIHEAAMALNNKVLHG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=466, Percent_Identity=42.274678111588, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI50301238, Length=467, Percent_Identity=25.9100642398287, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI291045266, Length=441, Percent_Identity=25.3968253968254, Blast_Score=119, Evalue=9e-27,
Organism=Homo sapiens, GI22035672, Length=471, Percent_Identity=26.5392781316348, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI33519430, Length=463, Percent_Identity=23.5421166306695, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33519428, Length=463, Percent_Identity=23.5421166306695, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33519426, Length=463, Percent_Identity=23.5421166306695, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI148277065, Length=463, Percent_Identity=23.5421166306695, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI148277071, Length=463, Percent_Identity=23.5421166306695, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI291045268, Length=433, Percent_Identity=24.2494226327945, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786307, Length=465, Percent_Identity=37.6344086021505, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI87082354, Length=472, Percent_Identity=27.1186440677966, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1789915, Length=416, Percent_Identity=26.2019230769231, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87081717, Length=469, Percent_Identity=26.0127931769723, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32565766, Length=469, Percent_Identity=42.2174840085288, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=26.5822784810127, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=441, Percent_Identity=26.984126984127, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=441, Percent_Identity=26.984126984127, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=483, Percent_Identity=26.7080745341615, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=184, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=40.0414937759336, Blast_Score=335, Evalue=1e-92,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=30.0847457627119, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=26.9807280513919, Blast_Score=147, Evalue=4e-36,
Organism=Drosophila melanogaster, GI21358499, Length=466, Percent_Identity=41.4163090128755, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24640553, Length=482, Percent_Identity=26.7634854771784, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24640549, Length=482, Percent_Identity=26.9709543568465, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24640551, Length=487, Percent_Identity=26.4887063655031, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17737741, Length=481, Percent_Identity=24.5322245322245, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49832; Mature: 49832

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIFDVAVIGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPSPGGTCLNVGCIPSKSL
CEEEEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCHHH
LQSSELYAQVQHEASIHGVNVEGVSFNAAAMIQRKDAIVSRLTMGISLLFKKNKVKHLCG
HHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHH
LATLECAQDEIWQLRVNDQHLHARNVVIATGSQPRQLPGVTIDNQQILDNRGALALSEVP
HHHHHHCCCCEEEEEECCCEEEECEEEEECCCCCCCCCCEEECCHHHHCCCCCEEECCCC
PRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPMFLPALEARLSNEVRKAMIASGMKMQL
CCCCEEECCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AVEIEAIEQRDDGVHVRWRQGEKQEESHFDKLILAIGRVPRLSGVDLVQLGLEADNRGGI
EEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCE
AVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGVVVADQIAGLAVEPVNFVLIPSVIYTQ
EHHHHHHCCCCCEEEHHHHHCCHHHHHHHHHCCEEEEHHHCCEEECCCCEEEECHHHCCC
PEVAWVGENEASLKAAGRAFNKGNSLFAGNGRALALGQEGGRCTLYSDKHSDRVLGGAIV
CCEEEECCCCHHHHHHHHHHCCCCEEEECCCCEEEEECCCCEEEEECCCCCCCEECCEEE
GPQASELINEIALAMTFSASGEDIACAVHAHPTLSEVIHEAAMALNNKVLHG
CCCHHHHHHHHHHEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MITIFDVAVIGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPSPGGTCLNVGCIPSKSL
CEEEEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCHHH
LQSSELYAQVQHEASIHGVNVEGVSFNAAAMIQRKDAIVSRLTMGISLLFKKNKVKHLCG
HHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHH
LATLECAQDEIWQLRVNDQHLHARNVVIATGSQPRQLPGVTIDNQQILDNRGALALSEVP
HHHHHHCCCCEEEEEECCCEEEECEEEEECCCCCCCCCCEEECCHHHHCCCCCEEECCCC
PRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPMFLPALEARLSNEVRKAMIASGMKMQL
CCCCEEECCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AVEIEAIEQRDDGVHVRWRQGEKQEESHFDKLILAIGRVPRLSGVDLVQLGLEADNRGGI
EEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCE
AVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGVVVADQIAGLAVEPVNFVLIPSVIYTQ
EHHHHHHCCCCCEEEHHHHHCCHHHHHHHHHCCEEEEHHHCCEEECCCCEEEECHHHCCC
PEVAWVGENEASLKAAGRAFNKGNSLFAGNGRALALGQEGGRCTLYSDKHSDRVLGGAIV
CCEEEECCCCHHHHHHHHHHCCCCEEEECCCCEEEEECCCCEEEEECCCCCCCEECCEEE
GPQASELINEIALAMTFSASGEDIACAVHAHPTLSEVIHEAAMALNNKVLHG
CCCHHHHHHHHHHEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]