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The map label for this gene is opuE [H]

Identifier: 218550365

GI number: 218550365

Start: 3131420

End: 3132889

Strand: Reverse

Name: opuE [H]

Synonym: EFER_3057

Alternate gene names: 218550365

Gene position: 3132889-3131420 (Counterclockwise)

Preceding gene: 218550366

Following gene: 218550363

Centisome position: 68.27

GC content: 50.2

Gene sequence:

>1470_bases
ATGAACAGTCATATCTTTTTAGTCGGCTTTATTATTTATGCCATCGCCATGATTTGGCTTGGTTGGTTTGTTTCTCGTAA
TCAGAAAAGCGGAGAAGATTTTCTGCTGGGCGGGCGTTCACTGCCGCTGTTTCTGACGCTGGGGTCAACCGTTGCCACGA
TGGTTGGCACGGGTTCCAGTATGGGCGCAGTGGGTTTTGGCTACAGCAATGGCTGGGCCGGAATGCTTTATGGCGTCGGT
GGGGCCGTAGGTATATTACTGGTGGCGTGGCTGTTTGCACCGGTACGTAAATTACGTTTTATGACCATGAGTGAAGAAAT
ATCTTATTATACTGGCGGCAGCCATTTAATTAAAAATATTGTCGGCCTGATGATATTTATTGCCTCTATTGGCTGGCTGG
GAGCGCATATATTAGGTGGCAGTATGTATCTTGCCTGGGCGACGGGTATTGATCTTACTGTAGCAAAATTAATTATCGCT
TTGGCCTTTGCGATTTACGTTATTATCGGCGGTTATTCGGCGGTAGTGTGGACCGATACCATTCAGGCCCTAATTTTGTT
CTTTGGCTTTATATTAATGGCTATTCTTGCCGTTGTTCACGTTGGTGGCTGGAGTGCGATTGAACAGGCGATGGACCCAA
AAGCGATGAGCCTGTTTGCGATTGATAAAATGGGCGTGCTGCCTGCGCTTTCGCTGGCGCTGGTTATTGGCGTAGGTGTG
CTGGCCACACCGTCTTATCGCCAGCGTATCTACTCGGGCAAAGATGTTCCCTCTGTACGCCGTTCGTTTGTTATTACTGG
CGTGTTGTATCTGTTCTTCTCGATTCTGCCTGCCATTATCGGCATGGCGGCGTTCACCATGAACCCAAATCTGGAAAACA
GCAACTACGCTTTCTTGTTCGCGACCAGCTTCTTACCTGCCATTCTTGGGCTGGTGGTGCTGATTGCCGGGCTTTCTGCC
ACCATGTCTTCTGCCAGTTCTGATGCCATTGCGGCAGTGGCGATTATGATGCGCGACGTCTACACCATGATTACCGGCAG
AATGCCGCCGGAGAACAAGGCTATTACTTACTCACGCTGGATGTTGACCTTTGTTATTGGCCTGGCGCTGGTCTTCGCTC
TGACCTCTAATGACATCATCAGCTACATTACCAAAATGATCTCGATGCTGATGTCCGGGCTGTTTATCTGCTCGATTCTC
GGTCGCTTCTGGCTACGCTTTAACTGGCAGGGCGCGCTGGCGGCGTTAGTGAGCGGTATGGTGATGTCAATTGTGGTGCT
GATGAATGCCGACTGGCTGGCTTACTGGGGTAACCCGTGTATTCCGTCAGTGCTGGGCAGCCTGGTCGCTGGCGTACTGG
TGACGTTGGTAACGCCAGCAAGTCAGGTCAGCCGGGAAGAAGCTTTGGCTATTATCACCAATGAGCGTGAAAACCAGAGT
GTCGTGATCACCAAACCTGAAGAAGCATAA

Upstream 100 bases:

>100_bases
AATTTAAGAATTAAAAAAACAGACCGCAATTGAGTGCTGAATACATTCAGCACTCCGGGAACTCTTACATTATTCTCTCT
CTACTGGCAGGATGTGAATT

Downstream 100 bases:

>100_bases
CCGACAATGCTAATCGCCAAGTGGCTGGCGGTTAGCGCCCCCACGGTGGCTGTCTAACACCAGTTTTATCCGCCGCAGGC
GATCTTTCGCCTGCGCTTTA

Product: Na+/proline symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLFLTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVG
GAVGILLVAWLFAPVRKLRFMTMSEEISYYTGGSHLIKNIVGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIA
LAFAIYVIIGGYSAVVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSLFAIDKMGVLPALSLALVIGVGV
LATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSILPAIIGMAAFTMNPNLENSNYAFLFATSFLPAILGLVVLIAGLSA
TMSSASSDAIAAVAIMMRDVYTMITGRMPPENKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMSGLFICSIL
GRFWLRFNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVAGVLVTLVTPASQVSREEALAIITNERENQS
VVITKPEEA

Sequences:

>Translated_489_residues
MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLFLTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVG
GAVGILLVAWLFAPVRKLRFMTMSEEISYYTGGSHLIKNIVGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIA
LAFAIYVIIGGYSAVVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSLFAIDKMGVLPALSLALVIGVGV
LATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSILPAIIGMAAFTMNPNLENSNYAFLFATSFLPAILGLVVLIAGLSA
TMSSASSDAIAAVAIMMRDVYTMITGRMPPENKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMSGLFICSIL
GRFWLRFNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVAGVLVTLVTPASQVSREEALAIITNERENQS
VVITKPEEA
>Mature_489_residues
MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLFLTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVG
GAVGILLVAWLFAPVRKLRFMTMSEEISYYTGGSHLIKNIVGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIA
LAFAIYVIIGGYSAVVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSLFAIDKMGVLPALSLALVIGVGV
LATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSILPAIIGMAAFTMNPNLENSNYAFLFATSFLPAILGLVVLIAGLSA
TMSSASSDAIAAVAIMMRDVYTMITGRMPPENKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMSGLFICSIL
GRFWLRFNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVAGVLVTLVTPASQVSREEALAIITNERENQS
VVITKPEEA

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI14140236, Length=471, Percent_Identity=25.0530785562633, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI4507031, Length=441, Percent_Identity=24.0362811791383, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI110835708, Length=486, Percent_Identity=21.1934156378601, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI109659836, Length=516, Percent_Identity=23.4496124031008, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1787251, Length=498, Percent_Identity=24.4979919678715, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082237, Length=453, Percent_Identity=23.1788079470199, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1790503, Length=444, Percent_Identity=21.6216216216216, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539284, Length=460, Percent_Identity=23.0434782608696, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 52568; Mature: 52568

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLFLTLGSTVATMVGTGSS
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEHHHHHHHHHCCCCC
MGAVGFGYSNGWAGMLYGVGGAVGILLVAWLFAPVRKLRFMTMSEEISYYTGGSHLIKNI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIALAFAIYVIIGGYSAVVWTDT
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSLFAIDKMGVLPALSLALVIGVGV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSILPAIIGMAAFTMNPNLENSNYAFLF
HCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEE
ATSFLPAILGLVVLIAGLSATMSSASSDAIAAVAIMMRDVYTMITGRMPPENKAITYSRW
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHH
MLTFVIGLALVFALTSNDIISYITKMISMLMSGLFICSILGRFWLRFNWQGALAALVSGM
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHH
VMSIVVLMNADWLAYWGNPCIPSVLGSLVAGVLVTLVTPASQVSREEALAIITNERENQS
HHHHHHHHCCCHHEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCCCCCE
VVITKPEEA
EEEECCCCC
>Mature Secondary Structure
MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLFLTLGSTVATMVGTGSS
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEHHHHHHHHHCCCCC
MGAVGFGYSNGWAGMLYGVGGAVGILLVAWLFAPVRKLRFMTMSEEISYYTGGSHLIKNI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIALAFAIYVIIGGYSAVVWTDT
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSLFAIDKMGVLPALSLALVIGVGV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSILPAIIGMAAFTMNPNLENSNYAFLF
HCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEE
ATSFLPAILGLVVLIAGLSATMSSASSDAIAAVAIMMRDVYTMITGRMPPENKAITYSRW
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHH
MLTFVIGLALVFALTSNDIISYITKMISMLMSGLFICSILGRFWLRFNWQGALAALVSGM
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHH
VMSIVVLMNADWLAYWGNPCIPSVLGSLVAGVLVTLVTPASQVSREEALAIITNERENQS
HHHHHHHHCCCHHEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCCCCCE
VVITKPEEA
EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]