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The map label for this gene is grdE [H]

Identifier: 218550323

GI number: 218550323

Start: 3084985

End: 3086274

Strand: Reverse

Name: grdE [H]

Synonym: EFER_3015

Alternate gene names: 218550323

Gene position: 3086274-3084985 (Counterclockwise)

Preceding gene: 218550324

Following gene: 218550322

Centisome position: 67.26

GC content: 50.93

Gene sequence:

>1290_bases
ATGAAACTGGAACTTGGCACTATTCAGATCAAGCAGTTCACTTTTGCAGAAAAAACATCACTGGCAGGCGGACATTTGCA
GCTCTGTCAGCGAGAACTTGAAGAGTTTATCCGCACCATTCACCCACAGATTGTGGAGGTGGAAACGGTGATTGTTCATC
CCGGTGAAGAAGTACGCATTATGCCGGTAAAAGATGTCATCGAACCACGCACCAAGCTGGATGATGGCTGCTGTTTTCCA
GGTATTAACCGGGGGGAAGAAGCTATTGTCGGGAGCGGTAAGACACTGGCCCTTAAAGGCATTTCGGTACTGACGACCGG
AAAAGTTGTAGGTTTCCAGGAAGGGATTATTGATATGTCTGGTCCTGGTGCGCCTTACACGCCTTATTCGACGTTGCGTA
ACCTGGTGCTGCGTATTGAAGTCGATGAGCAGTGCAACCAGTACCAGCATGAAGAGATCCTACGGCGTGTTGGGCTGATG
ACGGCACGTTGGCTTGCGGATCAGGTGAAAACGACTCCGGCGGATGAAGTCACAACCTACGAAACCTTGCCACTGGCGAA
GCAGCACGCAGCATATCCAGACCTGCCCGCAGTAGGCTACGTCTATATGCTACAAAGCCAGGGACTATTGCACGACACCT
GGTTTTACGGCGTAGATGTGAAAAAAATCGTCCCCACTCTGATGTATCCCACCGAAGCGATGGATGGCGCCATTGTCAGC
GGTAACTGTGTTTCTGCCTGCGATAAAAACACCACCTGGAGCCACCAGAACAATCCGGTGATTGCTGAGCTTTATCGCCG
TCATGGCAAAGATCTCAATTTCATTGGCGTTATTTTAACCAATGAAAACGTGACGTTGAGTGACAAAGTACGCTCATCAA
ATATGGTGGCGCGACTGGCAGATCAAATGGGGATTAGCGGGGCGATTATCAGTGAAGAAGGGTTTGGTAACCCGGATGCC
GATCTGATGATGAACTGCTACAAACTCGAAGCGCAGGGGATCCATACGGTGTTGTTAACCGACGAATATGCCGGGCGTGG
TGGGGAAAGCCAGTCGCTTGCCGACGCGCATCCTTCCGCCAATGCCGTGGTTACCGCTGGTAATGCCAATCAGGTCATTA
CTCTACCTCCGATGAAACGGGTGATAGGCCATTCGGTTTATGCCAATACCATCGCGGGTGGGTTTGAAGGCAGTTTGCAT
AACGACGGCAGTATTACTGTGGAAATACAAGCCATTACTGGCGCAACAAACGAGCTGGGGATTCACACCCTTTCGGCGCA
CAGCGGCTAA

Upstream 100 bases:

>100_bases
ACCGGCATGGGCTGTACCGGTCCGGTGTTGATGATTAATCGTCAACAACGTGCAGATGCTGATCAGCATCTGAAATCGTT
TTTGAGTTAAGGAGCCAGCC

Downstream 100 bases:

>100_bases
GGAGTGAGCGCAAATGAGTTTACGAATACTACATTACCTCAACCAGTTCTTTGGTCAAAAGGGCGGGGAAGAGGCGGCGG
GATGTCCGCCTGAGATCCAT

Product: glycine reductase complex component B subunits alpha/beta

Products: NA

Alternate protein names: Selenoprotein PB alpha/beta [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKLELGTIQIKQFTFAEKTSLAGGHLQLCQRELEEFIRTIHPQIVEVETVIVHPGEEVRIMPVKDVIEPRTKLDDGCCFP
GINRGEEAIVGSGKTLALKGISVLTTGKVVGFQEGIIDMSGPGAPYTPYSTLRNLVLRIEVDEQCNQYQHEEILRRVGLM
TARWLADQVKTTPADEVTTYETLPLAKQHAAYPDLPAVGYVYMLQSQGLLHDTWFYGVDVKKIVPTLMYPTEAMDGAIVS
GNCVSACDKNTTWSHQNNPVIAELYRRHGKDLNFIGVILTNENVTLSDKVRSSNMVARLADQMGISGAIISEEGFGNPDA
DLMMNCYKLEAQGIHTVLLTDEYAGRGGESQSLADAHPSANAVVTAGNANQVITLPPMKRVIGHSVYANTIAGGFEGSLH
NDGSITVEIQAITGATNELGIHTLSAHSG

Sequences:

>Translated_429_residues
MKLELGTIQIKQFTFAEKTSLAGGHLQLCQRELEEFIRTIHPQIVEVETVIVHPGEEVRIMPVKDVIEPRTKLDDGCCFP
GINRGEEAIVGSGKTLALKGISVLTTGKVVGFQEGIIDMSGPGAPYTPYSTLRNLVLRIEVDEQCNQYQHEEILRRVGLM
TARWLADQVKTTPADEVTTYETLPLAKQHAAYPDLPAVGYVYMLQSQGLLHDTWFYGVDVKKIVPTLMYPTEAMDGAIVS
GNCVSACDKNTTWSHQNNPVIAELYRRHGKDLNFIGVILTNENVTLSDKVRSSNMVARLADQMGISGAIISEEGFGNPDA
DLMMNCYKLEAQGIHTVLLTDEYAGRGGESQSLADAHPSANAVVTAGNANQVITLPPMKRVIGHSVYANTIAGGFEGSLH
NDGSITVEIQAITGATNELGIHTLSAHSG
>Mature_429_residues
MKLELGTIQIKQFTFAEKTSLAGGHLQLCQRELEEFIRTIHPQIVEVETVIVHPGEEVRIMPVKDVIEPRTKLDDGCCFP
GINRGEEAIVGSGKTLALKGISVLTTGKVVGFQEGIIDMSGPGAPYTPYSTLRNLVLRIEVDEQCNQYQHEEILRRVGLM
TARWLADQVKTTPADEVTTYETLPLAKQHAAYPDLPAVGYVYMLQSQGLLHDTWFYGVDVKKIVPTLMYPTEAMDGAIVS
GNCVSACDKNTTWSHQNNPVIAELYRRHGKDLNFIGVILTNENVTLSDKVRSSNMVARLADQMGISGAIISEEGFGNPDA
DLMMNCYKLEAQGIHTVLLTDEYAGRGGESQSLADAHPSANAVVTAGNANQVITLPPMKRVIGHSVYANTIAGGFEGSLH
NDGSITVEIQAITGATNELGIHTLSAHSG

Specific function: In the first step of glycine reductase, the substrate is bound to component PB via a Schiff base intermediate. Then the PB- activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated seleno

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016585
- InterPro:   IPR015417 [H]

Pfam domain/function: PF09338 Gly_reductase [H]

EC number: =1.21.4.2 [H]

Molecular weight: Translated: 46585; Mature: 46585

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLELGTIQIKQFTFAEKTSLAGGHLQLCQRELEEFIRTIHPQIVEVETVIVHPGEEVRI
CEEEEEEEEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEE
MPVKDVIEPRTKLDDGCCFPGINRGEEAIVGSGKTLALKGISVLTTGKVVGFQEGIIDMS
EEHHHHHCCHHCCCCCCCCCCCCCCCEEEECCCCEEEEECEEEEECCCEEECCCCCEECC
GPGAPYTPYSTLRNLVLRIEVDEQCNQYQHEEILRRVGLMTARWLADQVKTTPADEVTTY
CCCCCCCHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
ETLPLAKQHAAYPDLPAVGYVYMLQSQGLLHDTWFYGVDVKKIVPTLMYPTEAMDGAIVS
ECCCCHHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCHHCCCEEEE
GNCVSACDKNTTWSHQNNPVIAELYRRHGKDLNFIGVILTNENVTLSDKVRSSNMVARLA
CCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCEEEHHHHCCHHHHHHHH
DQMGISGAIISEEGFGNPDADLMMNCYKLEAQGIHTVLLTDEYAGRGGESQSLADAHPSA
HHCCCCEEEEECCCCCCCCHHHHHEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCC
NAVVTAGNANQVITLPPMKRVIGHSVYANTIAGGFEGSLHNDGSITVEIQAITGATNELG
CEEEEECCCCEEEECCCHHHHHCCHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCC
IHTLSAHSG
EEEECCCCC
>Mature Secondary Structure
MKLELGTIQIKQFTFAEKTSLAGGHLQLCQRELEEFIRTIHPQIVEVETVIVHPGEEVRI
CEEEEEEEEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEE
MPVKDVIEPRTKLDDGCCFPGINRGEEAIVGSGKTLALKGISVLTTGKVVGFQEGIIDMS
EEHHHHHCCHHCCCCCCCCCCCCCCCEEEECCCCEEEEECEEEEECCCEEECCCCCEECC
GPGAPYTPYSTLRNLVLRIEVDEQCNQYQHEEILRRVGLMTARWLADQVKTTPADEVTTY
CCCCCCCHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
ETLPLAKQHAAYPDLPAVGYVYMLQSQGLLHDTWFYGVDVKKIVPTLMYPTEAMDGAIVS
ECCCCHHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCHHCCCEEEE
GNCVSACDKNTTWSHQNNPVIAELYRRHGKDLNFIGVILTNENVTLSDKVRSSNMVARLA
CCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCEEEHHHHCCHHHHHHHH
DQMGISGAIISEEGFGNPDADLMMNCYKLEAQGIHTVLLTDEYAGRGGESQSLADAHPSA
HHCCCCEEEEECCCCCCCCHHHHHEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCC
NAVVTAGNANQVITLPPMKRVIGHSVYANTIAGGFEGSLHNDGSITVEIQAITGATNELG
CEEEEECCCCEEEECCCHHHHHCCHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCC
IHTLSAHSG
EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10091582; 8529639 [H]