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The map label for this gene is epsF [H]

Identifier: 218550225

GI number: 218550225

Start: 2986514

End: 2987737

Strand: Direct

Name: epsF [H]

Synonym: EFER_2917

Alternate gene names: 218550225

Gene position: 2986514-2987737 (Clockwise)

Preceding gene: 218550224

Following gene: 218550226

Centisome position: 65.08

GC content: 58.09

Gene sequence:

>1224_bases
ATGGCACTGTTTTACTATCAGGCGTTACAGCGTAATGGTCGCAAAACCAAAGGTATGATTGAGGCGGATTCCGCGCGTCA
TGCCCGCCAGTTGTTGCGCGGTAAAGAGCTTATCCCCGTACACATTGAAGCCCGGATGAATGCTTCGTCAGGGGGAATGT
TGCAGCGTCGGCAACACGCACATCGTCGTGTGGCGGCGGCAGATCTGGCGCTGTTCACTCGCCAACTGGCAACGCTGGTG
CAGGCAGCAATGCCGCTGGAAACCTGCTTACAGGCGGTCAGTGAGCAAAGTGAAAAACTGCATGTAAAAAGCCTCGGAAT
GGCGCTACGCAGCCGGATTCAGGAAGGTTACACCCTGTCGGACAGCCTGCGCGAACATCCCCGCGTCTTTGACTCCCTGT
TTTGTTCGATGGTGGCTGCCGGAGAAAAATCCGGGCATCTCGACGTGGTACTCAATCGCCTGGCGGATTACACCGAACAG
CGACAGCGCCTGAAATCACGCCTGCTGCAGGCCATGCTCTATCCGCTGGTACTGCTGGTGGTGGCAACGGGCGTGGTCAC
TATTTTGCTGACGGCGGTGGTGCCGAAAATTATCGAACAGTTTGATCATCTCGGACACGCGCTGCCTGCCTCCACCCGCG
CTCTTATCGCTATGAGCGACGCATTGCAGACCAGCGGCGTTTACTGGCTGGCGGGTCTGCTGGGGCTTCTGGTGCTGGGG
CAACGACTACTCAAAAATCCTGCGATGCGCCTGCGCTGGGATAAAACCTTGCTGTGTTTGCCCGTGACGGGGCGTGTTGC
GCGCGGGCTGAATACGGCGCGTTTTTCCCGCACGTTAAGCATCCTCACCGCCAGCAGTGTTCCGTTGCTGGAAGGCATTC
AGACCGCCGCTGCCGTGTCGGCAAATCGTTATGTCGAGCAACAACTGCTGCTGGCGGCAGATCGCGTCCGCGAAGGAAGC
AGCTTGCGCGCTGCGCTGGCGGAGTTGCGCCTGTTCCCGCCGATGATGCTGTACATGATCGCCTCCGGCGAACAGAGCGG
TGAACTGGAAACCATGCTTGAGCAGGCCGCAGTCAACCAGGAACGGGAGTTTGATACTCAGGTGGGGCTGGCGTTAGGGC
TGTTTGAACCGGCGCTGGTGGTAATGATGGCGGGCGTGGTGCTGTTTATCGTCATCGCCATCCTCGAGCCGATGCTGCAA
CTGAACAATATGGTTGGAATGTAA

Upstream 100 bases:

>100_bases
TCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTGCTTGCCGGAGAAACCTCTCTCGATGAAGTGCTG
CGGGTAACGATGGAGGCGTA

Downstream 100 bases:

>100_bases
TTTACGGAGTTATCACATGAATTCGTTATCCCGTACACAAAAACCACGGGCAGGTTTTACCCTGCTGGAAGTGATGGTGG
TGATTGTTATTCTTGGCGTC

Product: General secretion pathway protein F

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MALFYYQALQRNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNASSGGMLQRRQHAHRRVAAADLALFTRQLATLV
QAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRALIAMSDALQTSGVYWLAGLLGLLVLG
QRLLKNPAMRLRWDKTLLCLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGS
SLRAALAELRLFPPMMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQ
LNNMVGM

Sequences:

>Translated_407_residues
MALFYYQALQRNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNASSGGMLQRRQHAHRRVAAADLALFTRQLATLV
QAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRALIAMSDALQTSGVYWLAGLLGLLVLG
QRLLKNPAMRLRWDKTLLCLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGS
SLRAALAELRLFPPMMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQ
LNNMVGM
>Mature_406_residues
ALFYYQALQRNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNASSGGMLQRRQHAHRRVAAADLALFTRQLATLVQ
AAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQR
QRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRALIAMSDALQTSGVYWLAGLLGLLVLGQ
RLLKNPAMRLRWDKTLLCLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSS
LRAALAELRLFPPMMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQL
NNMVGM

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=403, Percent_Identity=44.9131513647643, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1786295, Length=409, Percent_Identity=25.4278728606357, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44813; Mature: 44681

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFYYQALQRNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNASSGGMLQRRQHA
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHH
HRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQRQRLKSRLLQAMLYPLVLLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATGVVTILLTAVVPKIIEQFDHLGHALPASTRALIAMSDALQTSGVYWLAGLLGLLVLG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
QRLLKNPAMRLRWDKTLLCLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVS
HHHHCCCCCEEEECCEEEEEECCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ANRYVEQQLLLAADRVREGSSLRAALAELRLFPPMMLYMIASGEQSGELETMLEQAAVNQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCC
EREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQLNNMVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALFYYQALQRNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNASSGGMLQRRQHA
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHH
HRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQRQRLKSRLLQAMLYPLVLLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATGVVTILLTAVVPKIIEQFDHLGHALPASTRALIAMSDALQTSGVYWLAGLLGLLVLG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
QRLLKNPAMRLRWDKTLLCLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVS
HHHHCCCCCEEEECCEEEEEECCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ANRYVEQQLLLAADRVREGSSLRAALAELRLFPPMMLYMIASGEQSGELETMLEQAAVNQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCC
EREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQLNNMVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]