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The map label for this gene is epsE [H]

Identifier: 218550224

GI number: 218550224

Start: 2985021

End: 2986514

Strand: Direct

Name: epsE [H]

Synonym: EFER_2916

Alternate gene names: 218550224

Gene position: 2985021-2986514 (Clockwise)

Preceding gene: 218550223

Following gene: 218550225

Centisome position: 65.05

GC content: 58.17

Gene sequence:

>1494_bases
ATGGTGCCTGTAGCACAGGAAACCACCGCCAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCATCGCTGGAGATCCTCCATGTGCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAGCAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCAATCATCAAACTTATCAACGCCATGTTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACATTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAACGCGTACC
GCAGGATGGGCGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGTATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGCCTGACGGGCGTGATGGCGCAGCGACTTGTTCGCACACTGTGCACCGACTGCCGCCAGCCCGCGCCTGCC
ACTGATGAAGAAAAACGCCTGCTGGGAATTACCGACGTGCGTACCGTCACGCTGTACCACCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGGCGTACTGCCATCCATGAGTTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTTGCCGGAGAAACCTCTCTCGATGAAGTGCTGCGGGTAACGATGGAGGCGTAA

Upstream 100 bases:

>100_bases
TAAGCCTGATGCCGCACACCGCGCAGCCGGTACTGCCAGCGCAAAATCAGGCTCTACCGCCGGAAGTTCGTGCGTTCCTT
AATGCCGGGAGAACGCGTTA

Downstream 100 bases:

>100_bases
TGGCACTGTTTTACTATCAGGCGTTACAGCGTAATGGTCGCAAAACCAAAGGTATGATTGAGGCGGATTCCGCGCGTCAT
GCCCGCCAGTTGTTGCGCGG

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCTDCRQPAPA
TDEEKRLLGITDVRTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Sequences:

>Translated_497_residues
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCTDCRQPAPA
TDEEKRLLGITDVRTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA
>Mature_497_residues
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCTDCRQPAPA
TDEEKRLLGITDVRTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=479, Percent_Identity=58.0375782881002, Blast_Score=563, Evalue=1e-162,
Organism=Escherichia coli, GI1786296, Length=384, Percent_Identity=46.3541666666667, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI87082188, Length=253, Percent_Identity=39.1304347826087, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55213; Mature: 55213

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLR
CCCCCCCCCCCEEECCCHHCCHHHEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCEEHH
QIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHH
INAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLRPGRKLASLLVSRIKVMARLD
HHHHHHHHHHCCCCCCEEEEECCEEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHH
IAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
HHHHCCCCCCCEEEEECCEEEEEEEECCCCHHHHHHHHHHHCCCCHHEEHHHHCCCEEHH
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQ
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCH
VNTRVGMTFARGLRAILRQDPDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGA
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEHHHCCHHHHH
ITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCTDCRQPAPATDEEKRLLGITDVRTVTLYH
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECEEEEEEEC
PQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHH
LAGETSLDEVLRVTMEA
HCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLR
CCCCCCCCCCCEEECCCHHCCHHHEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCEEHH
QIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHH
INAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLRPGRKLASLLVSRIKVMARLD
HHHHHHHHHHCCCCCCEEEEECCEEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHH
IAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
HHHHCCCCCCCEEEEECCEEEEEEEECCCCHHHHHHHHHHHCCCCHHEEHHHHCCCEEHH
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQ
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCH
VNTRVGMTFARGLRAILRQDPDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGA
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEHHHCCHHHHH
ITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCTDCRQPAPATDEEKRLLGITDVRTVTLYH
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECEEEEEEEC
PQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHH
LAGETSLDEVLRVTMEA
HCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]