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The map label for this gene is gdhA [H]

Identifier: 218548670

GI number: 218548670

Start: 1344223

End: 1345566

Strand: Reverse

Name: gdhA [H]

Synonym: EFER_1304

Alternate gene names: 218548670

Gene position: 1345566-1344223 (Counterclockwise)

Preceding gene: 218548672

Following gene: 218548665

Centisome position: 29.32

GC content: 51.86

Gene sequence:

>1344_bases
ATGGATCAGACATGTTCTCTGGAGTCCTTCCTCAACCATGTACAAAAGCGCGATCCGCATCAGACCGAATTCGCGCAAGC
CGTTCGTGAAGTAATGACCACCCTGTGGCCGTTTCTTGAGCAGAACCCACGTTATCGTCAAATGTCATTACTGGAACGTC
TGGTTGAACCAGAGCGTGCCATCCAGTTTCGCGTAGTTTGGGTTGATGATCGCGGTCAGGTACAAGTTAACCGTGCATGG
CGTGTTCAGTTCAACTCCGCTATTGGTCCGTATAAAGGCGGTATGCGTTTCCACCCATCTGTAAATCTTTCCATTCTTAA
ATTCCTTGGCTTCGAGCAAACCTTCAAAAACGCCCTGACCACTCTGCCTATGGGCGGCGGTAAAGGCGGAAGTGACTTCG
ATCCTAAAGGTAAAAGTGAAGGCGAAGTGATGCGTTTTTGTCAGGCACTGATGACGGAGCTCTATCGTCATCTGGGGCCA
GACACTGACGTTCCGGCAGGCGATATTGGCGTAGGCGGTCGTGAAGTTGGTTTTATGGCGGGTATGATGCGTAAGCTTTC
TAATAACAGCGCTTGCGTATTTACGGGTAAAGGTCTCTCTTTTGGCGGCAGCCTGATTCGACCAGAAGCTACTGGTTATG
GTCTGGTGTACTTCGCTGAAGCCATGCTGAAACGCCATGGTCTGGGCTTCGAAGGAATGCGTGTTTCGGTATCCGGCTCT
GGTAACGTTGCACAGTACGCAATCGAGAAAGCCATGGAATTCGGTGCCCGTGTGATCACCGCTTCTGATTCCAGCGGTAC
GGTCGTGGACGAAAGTGGCTTTACCAAAGAGAAACTGGCGCGCCTTATCGAAATCAAATCCAGTCGTGATGGTCGTGTTG
CAGATTACGCCAAAGAGTTTGGTCTGGTGTATCTCGAAGGCCAGCAGCCCTGGTCTGTTCCGGTTGATATCGCTCTGCCA
TGCGCCACCCAGAACGAACTGGATGTCGAAGCTGCTCAGGTTCTGATCGCCAATGGCGTGAAAGCGGTCGCGGAAGGTGC
CAACATGCCGACCACCATCGAAGCAACGAACCTGTTCCTTGAAGCAGGTGTACTGTTTGCACCGGGTAAAGCGGCCAATG
CCGGTGGCGTAGCGACCTCTGGTCTGGAAATGGCGCAGAACGCTGCTCGTTTAAGCTGGAAAGCCGAAAAAGTTGATGCT
CGCCTGCATCACATCATGCTCGATATTCACCATTCTTGTGTGGAATACGGCGGCGAAGGCAAGCAGACCAATTACGTGCA
GGGCGCGAACATTGCAGGCTTTGTAAAAGTAGCCGATGCAATGTTGAATCAGGGCGTGATTTAA

Upstream 100 bases:

>100_bases
TACCGTCACATTGCTGATGGTATAGTCGAAAACTGCAAATGCATTGAATAACAAACAGCATCAATAACATCACTCTAATA
TATAAGGGGTTTTTATATCT

Downstream 100 bases:

>100_bases
GTTGTAAATGCCTGATGGCGCTACGCTTATCGGGCCTACAAATGGGCATAATTCATTGCAATTACGCTCTAATGTAGGCC
GGGCAAGTGCAGCGCCCCCG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MDQTCSLESFLNHVQKRDPHQTEFAQAVREVMTTLWPFLEQNPRYRQMSLLERLVEPERAIQFRVVWVDDRGQVQVNRAW
RVQFNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGP
DTDVPAGDIGVGGREVGFMAGMMRKLSNNSACVFTGKGLSFGGSLIRPEATGYGLVYFAEAMLKRHGLGFEGMRVSVSGS
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALP
CATQNELDVEAAQVLIANGVKAVAEGANMPTTIEATNLFLEAGVLFAPGKAANAGGVATSGLEMAQNAARLSWKAEKVDA
RLHHIMLDIHHSCVEYGGEGKQTNYVQGANIAGFVKVADAMLNQGVI

Sequences:

>Translated_447_residues
MDQTCSLESFLNHVQKRDPHQTEFAQAVREVMTTLWPFLEQNPRYRQMSLLERLVEPERAIQFRVVWVDDRGQVQVNRAW
RVQFNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGP
DTDVPAGDIGVGGREVGFMAGMMRKLSNNSACVFTGKGLSFGGSLIRPEATGYGLVYFAEAMLKRHGLGFEGMRVSVSGS
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALP
CATQNELDVEAAQVLIANGVKAVAEGANMPTTIEATNLFLEAGVLFAPGKAANAGGVATSGLEMAQNAARLSWKAEKVDA
RLHHIMLDIHHSCVEYGGEGKQTNYVQGANIAGFVKVADAMLNQGVI
>Mature_447_residues
MDQTCSLESFLNHVQKRDPHQTEFAQAVREVMTTLWPFLEQNPRYRQMSLLERLVEPERAIQFRVVWVDDRGQVQVNRAW
RVQFNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGP
DTDVPAGDIGVGGREVGFMAGMMRKLSNNSACVFTGKGLSFGGSLIRPEATGYGLVYFAEAMLKRHGLGFEGMRVSVSGS
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALP
CATQNELDVEAAQVLIANGVKAVAEGANMPTTIEATNLFLEAGVLFAPGKAANAGGVATSGLEMAQNAARLSWKAEKVDA
RLHHIMLDIHHSCVEYGGEGKQTNYVQGANIAGFVKVADAMLNQGVI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=396, Percent_Identity=30.8080808080808, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI4885281, Length=390, Percent_Identity=30, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1788059, Length=447, Percent_Identity=94.407158836689, Blast_Score=880, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=345, Percent_Identity=30.7246376811594, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324951, Length=451, Percent_Identity=52.3281596452328, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=52.863436123348, Blast_Score=423, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24649283, Length=374, Percent_Identity=30.4812834224599, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45549226, Length=396, Percent_Identity=28.2828282828283, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI45553475, Length=381, Percent_Identity=28.8713910761155, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24649460, Length=321, Percent_Identity=30.2180685358255, Blast_Score=109, Evalue=5e-24,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48556; Mature: 48556

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQTCSLESFLNHVQKRDPHQTEFAQAVREVMTTLWPFLEQNPRYRQMSLLERLVEPERA
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
IQFRVVWVDDRGQVQVNRAWRVQFNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALT
EEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGPDTDVPAGDIGVGGREVGFMA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GMMRKLSNNSACVFTGKGLSFGGSLIRPEATGYGLVYFAEAMLKRHGLGFEGMRVSVSGS
HHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCC
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKSSRDGRVADYAKEF
CCHHHHHHHHHHHCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHC
GLVYLEGQQPWSVPVDIALPCATQNELDVEAAQVLIANGVKAVAEGANMPTTIEATNLFL
CEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCEEHHHHHEE
EAGVLFAPGKAANAGGVATSGLEMAQNAARLSWKAEKVDARLHHIMLDIHHSCVEYGGEG
ECCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
KQTNYVQGANIAGFVKVADAMLNQGVI
CCCCEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDQTCSLESFLNHVQKRDPHQTEFAQAVREVMTTLWPFLEQNPRYRQMSLLERLVEPERA
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
IQFRVVWVDDRGQVQVNRAWRVQFNSAIGPYKGGMRFHPSVNLSILKFLGFEQTFKNALT
EEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGPDTDVPAGDIGVGGREVGFMA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GMMRKLSNNSACVFTGKGLSFGGSLIRPEATGYGLVYFAEAMLKRHGLGFEGMRVSVSGS
HHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCC
GNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKSSRDGRVADYAKEF
CCHHHHHHHHHHHCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHC
GLVYLEGQQPWSVPVDIALPCATQNELDVEAAQVLIANGVKAVAEGANMPTTIEATNLFL
CEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCEEHHHHHEE
EAGVLFAPGKAANAGGVATSGLEMAQNAARLSWKAEKVDARLHHIMLDIHHSCVEYGGEG
ECCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
KQTNYVQGANIAGFVKVADAMLNQGVI
CCCCEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]