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The map label for this gene is nudG [H]

Identifier: 218548672

GI number: 218548672

Start: 1346152

End: 1346565

Strand: Reverse

Name: nudG [H]

Synonym: EFER_1306

Alternate gene names: 218548672

Gene position: 1346565-1346152 (Counterclockwise)

Preceding gene: 218548673

Following gene: 218548670

Centisome position: 29.35

GC content: 53.62

Gene sequence:

>414_bases
ATGAAAACAATCGATGTTGTTGCCGCCATTATCGAAAAAGATGGCAAAATCTTACTGGCTCAGCGCCCCGTTCATACAGA
TCAAGCCGGTTTATGGGAATTTGCCGGAGGTAAAGTGGAAGCGGGAGAAAGCCAACCGCAAGCGTTGATACGCGAGTTGC
ATGAGGAACTGGGGATCGACGCTCGCCCTGGTGACTACATTGCCAGCCACCAGCGGGAAGTCTCCGGGCGTATCATTCAT
CTTCATGCCTGGCATGTTCCTGAGTTCTACGGCACATTACAGGCCCATGAGCATCAGGCATTGGTCTGGTGTTCACCTGC
AGAAGCAATGGGCTATCCGCTGGCACCTGCCGACATCCCACTTCTGGAGGCATTTATGGCTTTACGCGGCGCCAGACCAG
CGGATTCGTACTGA

Upstream 100 bases:

>100_bases
TTGCAGACCGGGTAATACATTCACGCCGCATCCGGCATGAACAAGCGCACCTGGTCAGTAATCTGATATTATGGCATTTT
TGTGACTTATTCGTGTAGCT

Downstream 100 bases:

>100_bases
GAGTTTTCTCATCACGTTGGCACTGCAAGAGAACACCTTCCACTTTAATGACCGCGCCTTCTGAGTAGTTTTCGTCCTGG
TAGATACAACATTGATTACA

Product: pyrimidine (deoxy)nucleoside triphosphate pyrophosphohydrolase

Products: CMP; diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MKTIDVVAAIIEKDGKILLAQRPVHTDQAGLWEFAGGKVEAGESQPQALIRELHEELGIDARPGDYIASHQREVSGRIIH
LHAWHVPEFYGTLQAHEHQALVWCSPAEAMGYPLAPADIPLLEAFMALRGARPADSY

Sequences:

>Translated_137_residues
MKTIDVVAAIIEKDGKILLAQRPVHTDQAGLWEFAGGKVEAGESQPQALIRELHEELGIDARPGDYIASHQREVSGRIIH
LHAWHVPEFYGTLQAHEHQALVWCSPAEAMGYPLAPADIPLLEAFMALRGARPADSY
>Mature_137_residues
MKTIDVVAAIIEKDGKILLAQRPVHTDQAGLWEFAGGKVEAGESQPQALIRELHEELGIDARPGDYIASHQREVSGRIIH
LHAWHVPEFYGTLQAHEHQALVWCSPAEAMGYPLAPADIPLLEAFMALRGARPADSY

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=135, Percent_Identity=83.7037037037037, Blast_Score=232, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 15081; Mature: 15081

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIDVVAAIIEKDGKILLAQRPVHTDQAGLWEFAGGKVEAGESQPQALIRELHEELGID
CCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECCCCEEECCCCCHHHHHHHHHHHHCCC
ARPGDYIASHQREVSGRIIHLHAWHVPEFYGTLQAHEHQALVWCSPAEAMGYPLAPADIP
CCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCEEEEECCHHHCCCCCCCCCCH
LLEAFMALRGARPADSY
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTIDVVAAIIEKDGKILLAQRPVHTDQAGLWEFAGGKVEAGESQPQALIRELHEELGID
CCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECCCCEEECCCCCHHHHHHHHHHHHCCC
ARPGDYIASHQREVSGRIIHLHAWHVPEFYGTLQAHEHQALVWCSPAEAMGYPLAPADIP
CCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCEEEEECCHHHCCCCCCCCCCH
LLEAFMALRGARPADSY
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; H2O [C]

Specific reaction: CTP + H2O = CMP + diphosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]