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The map label for this gene is cmr [H]

Identifier: 218548361

GI number: 218548361

Start: 1015799

End: 1017031

Strand: Direct

Name: cmr [H]

Synonym: EFER_0983

Alternate gene names: 218548361

Gene position: 1015799-1017031 (Clockwise)

Preceding gene: 218548358

Following gene: 218548365

Centisome position: 22.14

GC content: 49.07

Gene sequence:

>1233_bases
ATGCAAAACCGATTACAAACCGGCGCACGACTGGGGCGTTCGGCGTTACTTTTTCCTCTTTGTCTGGTCCTGTACGAATT
CTCGACATATATCGCTAACGATATGATTCAGCCCGGCATGTTGGCTGTAGTGGAGCAATATCAGGCTGGAATTGACTGGG
TGCCAACGTCCATGACTGCCTATCTGGCAGGTGGCATGTTCTTACAATGGTTACTTGGGCCGTTGTCGGATCGTGTTGGT
CGCCGTCCGGTGATGCTGGTGGGGGTGGTGTGGTTTATCGTCACCTGTCTCGCCACGTTACTGGCACAAAATATTGAACA
ATTTACGGTGTTACGTTTTTTACAAGGAATTAGCCTCTGTTTTATTGGTGCTGTGGGCTATGCTGCAATTCAGGAGTCTT
TCGAAGAGTCGGTATGTATTAAAATTACAGCGCTTATGGCGAACGTCGCTCTGATTGCTCCTCTCCTTGGCCCTTTAGTA
GGGGCCGCCTGGGTGCATATGCTGCCCTGGGAGGGGATGTTTATCCTCTTTGCAGTGCTGGCGGCCATTTCCTTTGTGGG
CCTGCAGCGAGCAATGCCAGAAACAGCAACCCGTATCGGTGAAAAGCTCTCTTTAAAAGAGTTAGGTCGTGACTACAAAC
AGGTATTAAAGAATGGTCGCTTTGTAGCCGGAGCTCTGGCCCTGGGATTTGTTAGCTTGCCATTACTGGCGTGGATTGCC
CAATCACCCATTATCATTATCACGGGCGAGAAATTAAGCAGCTATGAATATGGCCTGTTACAGGTGCCTGTTTTTGGCGC
ACTGATTGCTGGCAACCTGGTACTGGCACGGTTAACTGCACGGCGCACAGTACGTTCGTTGATCATTATGGGTGGCTGGC
CGATTGTTATCGGGCTGGTCGTTGCTGCGATCGCGACGGCTTTCTCCTCACATGCCTATTTATGGATGACAGCGGGATTA
AGTATCTATGCTTTTGGTATTGGCCTGGCCAATGCCGGATTGGTTCGTTTGACCTTGTTTGCCAGTGATATGAGTAAAGG
TACAGTGTCGGCGGCCATGGGAATGGTACAGATGCTGATCTTTACGGTGGGAATTGAAGTCAGTAAACACGCATGGTTAG
GGGGTGGTAATGGCATGTTTAACCTGTTCAATCTGGCGAATGGTGTTATCTGGCTGCTGTTGATGGTGATTTTTTTACGT
GATAAAAGCGTTGGAAATTCACAGAAAGCTTAA

Upstream 100 bases:

>100_bases
TAACATTGTGCGTATTTAACGTTAAAAGCCCACTAATGACTATCTCTGCTGCGATACTTTCATTACACTTTGCGCGGATT
TTTATTAGTGAAGCGAATAG

Downstream 100 bases:

>100_bases
TGAAATGCCTGATAGCACAAAGCGATCAGGCACTTAATGATTAATGGTGATGGTCATCAATCTGATGTTCAATCACCATT
CCTTCACCTACGCTTGCAAG

Product: multidrug efflux system protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MQNRLQTGARLGRSALLFPLCLVLYEFSTYIANDMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRVG
RRPVMLVGVVWFIVTCLATLLAQNIEQFTVLRFLQGISLCFIGAVGYAAIQESFEESVCIKITALMANVALIAPLLGPLV
GAAWVHMLPWEGMFILFAVLAAISFVGLQRAMPETATRIGEKLSLKELGRDYKQVLKNGRFVAGALALGFVSLPLLAWIA
QSPIIIITGEKLSSYEYGLLQVPVFGALIAGNLVLARLTARRTVRSLIIMGGWPIVIGLVVAAIATAFSSHAYLWMTAGL
SIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMVQMLIFTVGIEVSKHAWLGGGNGMFNLFNLANGVIWLLLMVIFLR
DKSVGNSQKA

Sequences:

>Translated_410_residues
MQNRLQTGARLGRSALLFPLCLVLYEFSTYIANDMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRVG
RRPVMLVGVVWFIVTCLATLLAQNIEQFTVLRFLQGISLCFIGAVGYAAIQESFEESVCIKITALMANVALIAPLLGPLV
GAAWVHMLPWEGMFILFAVLAAISFVGLQRAMPETATRIGEKLSLKELGRDYKQVLKNGRFVAGALALGFVSLPLLAWIA
QSPIIIITGEKLSSYEYGLLQVPVFGALIAGNLVLARLTARRTVRSLIIMGGWPIVIGLVVAAIATAFSSHAYLWMTAGL
SIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMVQMLIFTVGIEVSKHAWLGGGNGMFNLFNLANGVIWLLLMVIFLR
DKSVGNSQKA
>Mature_410_residues
MQNRLQTGARLGRSALLFPLCLVLYEFSTYIANDMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRVG
RRPVMLVGVVWFIVTCLATLLAQNIEQFTVLRFLQGISLCFIGAVGYAAIQESFEESVCIKITALMANVALIAPLLGPLV
GAAWVHMLPWEGMFILFAVLAAISFVGLQRAMPETATRIGEKLSLKELGRDYKQVLKNGRFVAGALALGFVSLPLLAWIA
QSPIIIITGEKLSSYEYGLLQVPVFGALIAGNLVLARLTARRTVRSLIIMGGWPIVIGLVVAAIATAFSSHAYLWMTAGL
SIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMVQMLIFTVGIEVSKHAWLGGGNGMFNLFNLANGVIWLLLMVIFLR
DKSVGNSQKA

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=409, Percent_Identity=90.7090464547677, Blast_Score=756, Evalue=0.0,
Organism=Escherichia coli, GI1790794, Length=378, Percent_Identity=42.0634920634921, Blast_Score=321, Evalue=5e-89,
Organism=Escherichia coli, GI48994889, Length=217, Percent_Identity=26.2672811059908, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788509, Length=304, Percent_Identity=23.3552631578947, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790146, Length=143, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87082312, Length=171, Percent_Identity=32.7485380116959, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44259; Mature: 44259

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure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HHHCCHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]