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The map label for this gene is vanB [H]

Identifier: 218548184

GI number: 218548184

Start: 819063

End: 819995

Strand: Direct

Name: vanB [H]

Synonym: EFER_0801

Alternate gene names: 218548184

Gene position: 819063-819995 (Clockwise)

Preceding gene: 218548183

Following gene: 218548185

Centisome position: 17.85

GC content: 61.09

Gene sequence:

>933_bases
ATGCTGACACTGCATATTATTAAGCGCGAGCTTCAGGGGGATGTCGTCCTGCTGACGCTTGCCGACGCAACCGGCGCTGA
ACTGCCTGCGTTCACCGCCGGGGCGCACGTCGACCTGCATCTGACCGACGATCTGGTGCGCCCGTATTCGCTCTGCGGCG
ATCCAGCAGATCGCCACTGCTATCAACTGGGCATCCTCAAAGATAGCCAGTCGAAAGGCGGATCGCTGGCGGCACACGCG
CTTCAGGAAGGCGAATCGGTAACGGTGAGCCTGCCGCGCAATCTGTTTGCCCTTGATGCGCGCGCGGCGCACAGCCTGCT
GATCGGCGGCGGGATTGGTATCACGCCGATGCTGGCGATGGCGGCGGAGCTGCACGCCGCCGGGCGCGCGTTTTCGCTGC
ATTACTGCGCCCGTTCGCGTCCGCAGGCGGCGTTTGTTGCCGAACTGGAAAAAGCGCCGTGGGCGGATCGCGTCTGGCTG
CATTTCAGTGAGGAACAACGCATTGATTTGCCCGCGGTACTGAGTGATGTACCGGCTAACACCCATATCTATGTTTGTGG
CCCCACGCGGCTGATGGAGGCGGTGAGCGAGCAGGCAAACGCCGCCGGTTATGCGGCTGAAAACGTCCATCAGGAGTGCT
TTAGCGCGGAAGTTGAAGTGAGCGGAAAGGCGTTTGACGTGGTGGCAGCAACCAGTGGGATTACCGTGCGGGTGCTGGAA
AACCAGACCATCGTCGAGGCACTGGCGCAGGCCGGGTTGAAGGTGTGCGTCTCCTGTAAGCAGGGGATTTGCGGCAGTTG
CCTGACGGATGTGATTGAGGGCGAGCCGGATCACCGCGACAGCTATCTGACAGACGAGGAGAAAGCCGATGGCGACCAGA
TCCTGCTCTGTTGTTCCCGGGCGAAAAGTGCCCGTCTGATTATCGATCTCTGA

Upstream 100 bases:

>100_bases
CGCTCAGGCCGTGGCACTGCTGGCTTCACCGCAGGCGGCGTTTGTCACCGGGGCGACCTGGGACATCAACGGCGGTCTGT
TCATGCGTTAAGGAGTCGCC

Downstream 100 bases:

>100_bases
GGATTTCAGCATGAATTTGCAGACCGAGTTTCGTAATGCGATGGCGCAACTGGGCAGCGCGGTGTCGGTGATCACCACCG
ATGGCCCGGCGGGGAAATTT

Product: Vanillate O-demethylase oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MLTLHIIKRELQGDVVLLTLADATGAELPAFTAGAHVDLHLTDDLVRPYSLCGDPADRHCYQLGILKDSQSKGGSLAAHA
LQEGESVTVSLPRNLFALDARAAHSLLIGGGIGITPMLAMAAELHAAGRAFSLHYCARSRPQAAFVAELEKAPWADRVWL
HFSEEQRIDLPAVLSDVPANTHIYVCGPTRLMEAVSEQANAAGYAAENVHQECFSAEVEVSGKAFDVVAATSGITVRVLE
NQTIVEALAQAGLKVCVSCKQGICGSCLTDVIEGEPDHRDSYLTDEEKADGDQILLCCSRAKSARLIIDL

Sequences:

>Translated_310_residues
MLTLHIIKRELQGDVVLLTLADATGAELPAFTAGAHVDLHLTDDLVRPYSLCGDPADRHCYQLGILKDSQSKGGSLAAHA
LQEGESVTVSLPRNLFALDARAAHSLLIGGGIGITPMLAMAAELHAAGRAFSLHYCARSRPQAAFVAELEKAPWADRVWL
HFSEEQRIDLPAVLSDVPANTHIYVCGPTRLMEAVSEQANAAGYAAENVHQECFSAEVEVSGKAFDVVAATSGITVRVLE
NQTIVEALAQAGLKVCVSCKQGICGSCLTDVIEGEPDHRDSYLTDEEKADGDQILLCCSRAKSARLIIDL
>Mature_310_residues
MLTLHIIKRELQGDVVLLTLADATGAELPAFTAGAHVDLHLTDDLVRPYSLCGDPADRHCYQLGILKDSQSKGGSLAAHA
LQEGESVTVSLPRNLFALDARAAHSLLIGGGIGITPMLAMAAELHAAGRAFSLHYCARSRPQAAFVAELEKAPWADRVWL
HFSEEQRIDLPAVLSDVPANTHIYVCGPTRLMEAVSEQANAAGYAAENVHQECFSAEVEVSGKAFDVVAATSGITVRVLE
NQTIVEALAQAGLKVCVSCKQGICGSCLTDVIEGEPDHRDSYLTDEEKADGDQILLCCSRAKSARLIIDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=306, Percent_Identity=41.8300653594771, Blast_Score=230, Evalue=9e-62,
Organism=Escherichia coli, GI1787658, Length=321, Percent_Identity=23.98753894081, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 33095; Mature: 33095

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLHIIKRELQGDVVLLTLADATGAELPAFTAGAHVDLHLTDDLVRPYSLCGDPADRHC
CEEHEEEHHHHCCCEEEEEEECCCCCCCCCEECCCEEEEEECHHHHCHHHHCCCCCCCCE
YQLGILKDSQSKGGSLAAHALQEGESVTVSLPRNLFALDARAAHSLLIGGGIGITPMLAM
EEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCEEEECHHHHCEEEEECCCCHHHHHHH
AAELHAAGRAFSLHYCARSRPQAAFVAELEKAPWADRVWLHFSEEQRIDLPAVLSDVPAN
HHHHHHCCCEEEEEEHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHCCCCC
THIYVCGPTRLMEAVSEQANAAGYAAENVHQECFSAEVEVSGKAFDVVAATSGITVRVLE
CEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCEEEEEEECCCCEEEEEC
NQTIVEALAQAGLKVCVSCKQGICGSCLTDVIEGEPDHRDSYLTDEEKADGDQILLCCSR
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECC
AKSARLIIDL
CCCCEEEEEC
>Mature Secondary Structure
MLTLHIIKRELQGDVVLLTLADATGAELPAFTAGAHVDLHLTDDLVRPYSLCGDPADRHC
CEEHEEEHHHHCCCEEEEEEECCCCCCCCCEECCCEEEEEECHHHHCHHHHCCCCCCCCE
YQLGILKDSQSKGGSLAAHALQEGESVTVSLPRNLFALDARAAHSLLIGGGIGITPMLAM
EEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCEEEECHHHHCEEEEECCCCHHHHHHH
AAELHAAGRAFSLHYCARSRPQAAFVAELEKAPWADRVWLHFSEEQRIDLPAVLSDVPAN
HHHHHHCCCEEEEEEHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHCCCCC
THIYVCGPTRLMEAVSEQANAAGYAAENVHQECFSAEVEVSGKAFDVVAATSGITVRVLE
CEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCEEEEEEECCCCEEEEEC
NQTIVEALAQAGLKVCVSCKQGICGSCLTDVIEGEPDHRDSYLTDEEKADGDQILLCCSR
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECC
AKSARLIIDL
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3170489 [H]