Click here to switch to the map view.

The map label for this gene is pcnB [H]

Identifier: 218547599

GI number: 218547599

Start: 179715

End: 181133

Strand: Reverse

Name: pcnB [H]

Synonym: EFER_0166

Alternate gene names: 218547599

Gene position: 181133-179715 (Counterclockwise)

Preceding gene: 218547600

Following gene: 218547598

Centisome position: 3.95

GC content: 53.56

Gene sequence:

>1419_bases
TTGACACTACCGAGGTGTACTATTTTTACCCGAGTCGCTAATTTTTGCCGCAAGGTGCTAAGCCGCGAGGAAAGCGAGGC
TGAACAGGCTGCCGCCCGTCCACAGGTGACGGTGATCCCGCGTGAGCAACATGCTATCTCTCGCAAAGATATCAGTGAAA
ACGCTCTGAAGGTAATGTACAGGCTCAATAAAGCGGGATACGAAGCCTGGTTGGTTGGCGGTGGTGTACGCGACCTGTTA
CTTGGCAAAAAACCAAAAGATTTTGATGTCACCACCAACGCCACGCCGGAGCAGGTTCGCAAACTGTTCCGTAACTGCCG
CCTGGTAGGTCGCCGTTTCCGTCTTGCTCATGTCATGTTTGGTCCAGAGATTATCGAAGTCGCAACCTTCCGTGGACACC
ACGAAGGTAATGTCAGCGACCGCACGACATCCCAACGCGGGCAAAACGGCATGTTGCTGCGCGACAACATTTTCGGTTCC
ATCGAAGAAGACGCCCAGCGCCGCGATTTCACCATCAATAGCCTGTATTACAGCGTGGCGGATTTCACCGTACGCGATTA
CGTTGGTGGGATGCAAGACCTGAAAGAAGGCATTATTCGGCTGATTGGTAACCCGGAAACGCGCTACCGTGAAGATCCGG
TACGTATGCTACGTGCCGTGCGTTTTGCCGCGAAACTAAACATGCAAATCAGCCCGGAAACCGCCGAGCCAATCCCGCGC
CTGGCCGCATTGCTCAACGATGTACCGCCAGCTCGCCTGTTTGAAGAATCTCTTAAACTGTTGCAGGCAGGTTACGGTTA
CGAAACCTACAAGCTGCTGCGCGAATACCATCTGTTCCAGCCGTTGTTCCCGACCATTACTCGTTACTTCACCGAAAACG
GTGATAGCGCAATGGAACGGATGATTATTCAGGTGTTGAAGAACACCGATAACCGTATTCACAACGGTCTGCGCGTGAAT
CCTGCGTTCCTTTTTGCAGCGATGTTCTGGTATCCGTTAATGGAGACAGCACAACGCATTGCCCAGGAGAGCGGTCTGAC
CTGGTCAGATGCCTTTGCACTGGCAATGAATGATGTACTGGACGAAGCCTGCCGTTCGCTGGCGATCCCTAAACGTCTCA
CTACGTTAACCCGCGATATCTGGCAATTGCAGTTACGCATGTCGCGCCGCCAGGGTAAACGTGCATGGAAGCTGCTGGAG
CATCCAAAATTCCGTGCGGCTTATGATCTTCTGGCACTTCGTGCCGAAGTTGAGCGCAATTCTGAATTGCAACGCCTGGC
AAAATGGTGGGGTGAATTTCAGGTTTCTGCGCCACCAGAACAAAAAGGCATGCTTAATGAGCTTGACGAAGATCCGGCAC
CGCGCCGCCGTCATCGTCGTCCACGCCGACGTGCCCCGCGTCGTGAGGGTACGGCGTGA

Upstream 100 bases:

>100_bases
GGCTCACCACAGTGCCAGAGTCAGCAATTGTAAATTCAACATTCTCAAATGCGTCATGCTGAGCTATGATTAGCCGCTAT
TTTTTTGTCCTGAATGATGT

Downstream 100 bases:

>100_bases
CCATCGCCTATATCGCCATCGGCAGCAATCTGGCCTCTCCGCTGGAGCAGGTCAATGCTGCCGTCAAAGCACTTAGCGAA
ATTCCTCAAAGCAAAATTCT

Product: poly(A) polymerase I

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTLPRCTIFTRVANFCRKVLSREESEAEQAAARPQVTVIPREQHAISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLL
LGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
IEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLKEGIIRLIGNPETRYREDPVRMLRAVRFAAKLNMQISPETAEPIPR
LAALLNDVPPARLFEESLKLLQAGYGYETYKLLREYHLFQPLFPTITRYFTENGDSAMERMIIQVLKNTDNRIHNGLRVN
PAFLFAAMFWYPLMETAQRIAQESGLTWSDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLE
HPKFRAAYDLLALRAEVERNSELQRLAKWWGEFQVSAPPEQKGMLNELDEDPAPRRRHRRPRRRAPRREGTA

Sequences:

>Translated_472_residues
MTLPRCTIFTRVANFCRKVLSREESEAEQAAARPQVTVIPREQHAISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLL
LGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
IEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLKEGIIRLIGNPETRYREDPVRMLRAVRFAAKLNMQISPETAEPIPR
LAALLNDVPPARLFEESLKLLQAGYGYETYKLLREYHLFQPLFPTITRYFTENGDSAMERMIIQVLKNTDNRIHNGLRVN
PAFLFAAMFWYPLMETAQRIAQESGLTWSDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLE
HPKFRAAYDLLALRAEVERNSELQRLAKWWGEFQVSAPPEQKGMLNELDEDPAPRRRHRRPRRRAPRREGTA
>Mature_471_residues
TLPRCTIFTRVANFCRKVLSREESEAEQAAARPQVTVIPREQHAISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLL
GKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSI
EEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLKEGIIRLIGNPETRYREDPVRMLRAVRFAAKLNMQISPETAEPIPRL
AALLNDVPPARLFEESLKLLQAGYGYETYKLLREYHLFQPLFPTITRYFTENGDSAMERMIIQVLKNTDNRIHNGLRVNP
AFLFAAMFWYPLMETAQRIAQESGLTWSDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLEH
PKFRAAYDLLALRAEVERNSELQRLAKWWGEFQVSAPPEQKGMLNELDEDPAPRRRHRRPRRRAPRREGTA

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=274, Percent_Identity=31.021897810219, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI87081691, Length=465, Percent_Identity=94.1935483870968, Blast_Score=874, Evalue=0.0,
Organism=Escherichia coli, GI1789436, Length=229, Percent_Identity=31.4410480349345, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=233, Percent_Identity=27.4678111587983, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6321016, Length=277, Percent_Identity=24.9097472924188, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281360112, Length=240, Percent_Identity=30.8333333333333, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21357337, Length=240, Percent_Identity=30.8333333333333, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 54663; Mature: 54531

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPRCTIFTRVANFCRKVLSREESEAEQAAARPQVTVIPREQHAISRKDISENALKVMY
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHH
RLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMF
HHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVA
CCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHH
DFTVRDYVGGMQDLKEGIIRLIGNPETRYREDPVRMLRAVRFAAKLNMQISPETAEPIPR
HHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCEECCCCCCHHHH
LAALLNDVPPARLFEESLKLLQAGYGYETYKLLREYHLFQPLFPTITRYFTENGDSAMER
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
MIIQVLKNTDNRIHNGLRVNPAFLFAAMFWYPLMETAQRIAQESGLTWSDAFALAMNDVL
HHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLEHPKFRAAYDLLALRAEVERN
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
SELQRLAKWWGEFQVSAPPEQKGMLNELDEDPAPRRRHRRPRRRAPRREGTA
HHHHHHHHHHCCCCCCCCCHHCCHHHHHCCCCCCHHHHCCHHHCCCCCCCCC
>Mature Secondary Structure 
TLPRCTIFTRVANFCRKVLSREESEAEQAAARPQVTVIPREQHAISRKDISENALKVMY
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHH
RLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMF
HHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVA
CCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHH
DFTVRDYVGGMQDLKEGIIRLIGNPETRYREDPVRMLRAVRFAAKLNMQISPETAEPIPR
HHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCEECCCCCCHHHH
LAALLNDVPPARLFEESLKLLQAGYGYETYKLLREYHLFQPLFPTITRYFTENGDSAMER
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
MIIQVLKNTDNRIHNGLRVNPAFLFAAMFWYPLMETAQRIAQESGLTWSDAFALAMNDVL
HHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLEHPKFRAAYDLLALRAEVERN
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
SELQRLAKWWGEFQVSAPPEQKGMLNELDEDPAPRRRHRRPRRRAPRREGTA
HHHHHHHHHHCCCCCCCCCHHCCHHHHHCCCCCCHHHHCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]