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The map label for this gene is yadB [H]

Identifier: 218547600

GI number: 218547600

Start: 181172

End: 182080

Strand: Reverse

Name: yadB [H]

Synonym: EFER_0167

Alternate gene names: 218547600

Gene position: 182080-181172 (Counterclockwise)

Preceding gene: 218547601

Following gene: 218547599

Centisome position: 3.97

GC content: 51.05

Gene sequence:

>909_bases
TTGTTCAAAGAGATGACTGACACACATTATATTGGCCGCTTCGCCCCCTCACCTTCCGGCGAGCTCCATTTTGGTTCTCT
GATTGCCGCGCTCGGCAGTTTTCTTCAGGCTCGTGCCCAACACGGTCATTGGTTAGTACGCATTGAAGATATTGATCCAC
CGCGTGAAGTTCCCGGTGCCGCTGATACCATTCTGCGCCAACTGGAACATTACGGGCTGCACTGGGATGGCGAAGTTCTT
TGGCAATCACAACGTCACGATGCCTACCGTGAAGCGCTAGCCTGGTTACAGCAACAAGGGCTGAGCTACTATTGCACCTG
TACCCGTGCACGGGTGCAGAGCGTCGGCGGAATTTACGACGGGCATTGTCGTGAGCTGCATCATGGACCTGAACATGCAG
CTGTGCGAATAAAACAGACGCACCCAGTGACACAATTTACCGATCGCCTGCGTGGTGTTATTCACGCTGACGAAAAACTG
GCCCGGGAAGATTTTATAATTCATCGCCGTGATGGGTTGTTCGCCTACAACCTGGCTGTTGTGGTTGATGATCATTTCCA
GGGCGTTACAGAAATAGTGCGTGGGGCTGATCTGATTGAACCGACAGTAAGGCAAATTTCGCTGTACCAACTCTTTGGCT
GGCAAGTACCGGATTACATCCATTTACCGCTGGCGCTAAATCAACAAGGCGCTAAACTTTCCAAGCAGAATCATGCGCCT
GCGTTGCCGAAAGGTGATCCACGCCCGGTACTAATCGCGGCGTTACGTTTCCTTGGGCAACCTGAGTTTCCGTCGTGGCA
AGATCTCAGTGTTAATGAATTATTACAAAGTGCAGTGAACAACTGGCGGCTCACCACAGTGCCAGAGTCAGCAATTGTAA
ATTCAACATTCTCAAATGCGTCATGCTGA

Upstream 100 bases:

>100_bases
ACTGCAAAACGCTGGCTGAAATTCGCGAAAAACAGATGGCGGGCTAAGCCAACCGTTTCATCACGTTTACCACAGGCGGG
AATTACTCCCGCCTTATTTT

Downstream 100 bases:

>100_bases
GCTATGATTAGCCGCTATTTTTTTGTCCTGAATGATGTTTGACACTACCGAGGTGTACTATTTTTACCCGAGTCGCTAAT
TTTTGCCGCAAGGTGCTAAG

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSFLQARAQHGHWLVRIEDIDPPREVPGAADTILRQLEHYGLHWDGEVL
WQSQRHDAYREALAWLQQQGLSYYCTCTRARVQSVGGIYDGHCRELHHGPEHAAVRIKQTHPVTQFTDRLRGVIHADEKL
AREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWQVPDYIHLPLALNQQGAKLSKQNHAP
ALPKGDPRPVLIAALRFLGQPEFPSWQDLSVNELLQSAVNNWRLTTVPESAIVNSTFSNASC

Sequences:

>Translated_302_residues
MFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSFLQARAQHGHWLVRIEDIDPPREVPGAADTILRQLEHYGLHWDGEVL
WQSQRHDAYREALAWLQQQGLSYYCTCTRARVQSVGGIYDGHCRELHHGPEHAAVRIKQTHPVTQFTDRLRGVIHADEKL
AREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWQVPDYIHLPLALNQQGAKLSKQNHAP
ALPKGDPRPVLIAALRFLGQPEFPSWQDLSVNELLQSAVNNWRLTTVPESAIVNSTFSNASC
>Mature_302_residues
MFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSFLQARAQHGHWLVRIEDIDPPREVPGAADTILRQLEHYGLHWDGEVL
WQSQRHDAYREALAWLQQQGLSYYCTCTRARVQSVGGIYDGHCRELHHGPEHAAVRIKQTHPVTQFTDRLRGVIHADEKL
AREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWQVPDYIHLPLALNQQGAKLSKQNHAP
ALPKGDPRPVLIAALRFLGQPEFPSWQDLSVNELLQSAVNNWRLTTVPESAIVNSTFSNASC

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=250, Percent_Identity=35.2, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI226510925, Length=302, Percent_Identity=89.0728476821192, Blast_Score=563, Evalue=1e-162,
Organism=Escherichia coli, GI1788743, Length=244, Percent_Identity=35.2459016393443, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71993394, Length=250, Percent_Identity=31.6, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324540, Length=250, Percent_Identity=34.4, Blast_Score=142, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24665321, Length=249, Percent_Identity=35.3413654618474, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: NA

Molecular weight: Translated: 34128; Mature: 34128

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSFLQARAQHGHWLVRIEDIDPPREVPGA
CCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCH
ADTILRQLEHYGLHWDGEVLWQSQRHDAYREALAWLQQQGLSYYCTCTRARVQSVGGIYD
HHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCC
GHCRELHHGPEHAAVRIKQTHPVTQFTDRLRGVIHADEKLAREDFIIHRRDGLFAYNLAV
CHHHHHCCCCCHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCEEEEEEEE
VVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWQVPDYIHLPLALNQQGAKLSKQNHAP
EECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEECCEEECCCCCHHCCCCCCC
ALPKGDPRPVLIAALRFLGQPEFPSWQDLSVNELLQSAVNNWRLTTVPESAIVNSTFSNA
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCEEEECCHHHHHCCCCCCC
SC
CC
>Mature Secondary Structure
MFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSFLQARAQHGHWLVRIEDIDPPREVPGA
CCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCH
ADTILRQLEHYGLHWDGEVLWQSQRHDAYREALAWLQQQGLSYYCTCTRARVQSVGGIYD
HHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCC
GHCRELHHGPEHAAVRIKQTHPVTQFTDRLRGVIHADEKLAREDFIIHRRDGLFAYNLAV
CHHHHHCCCCCHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCEEEEEEEE
VVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWQVPDYIHLPLALNQQGAKLSKQNHAP
EECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEECCEEECCCCCHHCCCCCCC
ALPKGDPRPVLIAALRFLGQPEFPSWQDLSVNELLQSAVNNWRLTTVPESAIVNSTFSNA
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCEEEECCHHHHHCCCCCCC
SC
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]