Click here to switch to the map view.

The map label for this gene is folK [H]

Identifier: 218547598

GI number: 218547598

Start: 179239

End: 179718

Strand: Reverse

Name: folK [H]

Synonym: EFER_0165

Alternate gene names: 218547598

Gene position: 179718-179239 (Counterclockwise)

Preceding gene: 218547599

Following gene: 218547597

Centisome position: 3.92

GC content: 49.17

Gene sequence:

>480_bases
GTGACCATCGCCTATATCGCCATCGGCAGCAATCTGGCCTCTCCGCTGGAGCAGGTCAATGCTGCCGTCAAAGCACTTAG
CGAAATTCCTCAAAGCAAAATTCTTGCTGTTTCCTCTTTTTACCGCACCCCGCCGCTGGGGCCACAAGATCAACCTGATT
ACCTCAACGCCGCCGTGGCGCTTGAAACCTCCCTCGCCCCGGAAGAGTTACTCAACCATACACAGCGCATAGAGCTGCAA
CAGGGTCGTGTTCGTAAAGCTGAACGCTGGGGACCACGCACGCTGGATCTCGACATCATGCTGTTTGGTAATGAAGTGAT
AAATACCGAACGTCTGACCGTTCCGCACTACGATATGAAAAATCGTGGCTTTATGTTGTGGCCACTGTTTGAGATTGCGC
CGGAGCTGGTTTTTCCTGATGGTTTATTTTTAAATAAGCTCTTACATAAAAAAGGTTTTACTAAACCTGACAAATGGTAA

Upstream 100 bases:

>100_bases
CAGAACAAAAAGGCATGCTTAATGAGCTTGACGAAGATCCGGCACCGCGCCGCCGTCATCGTCGTCCACGCCGACGTGCC
CCGCGTCGTGAGGGTACGGC

Downstream 100 bases:

>100_bases
TTCATCATTTTAGTGAATATAAATTTATTCTGAAAATCTAATTCGATGCATTGCCAATTAATGTAATCTGATATAAATAA
AAATTTGCGAAACAATATAT

Product: 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTIAYIAIGSNLASPLEQVNAAVKALSEIPQSKILAVSSFYRTPPLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQ
QGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLFLNKLLHKKGFTKPDKW

Sequences:

>Translated_159_residues
MTIAYIAIGSNLASPLEQVNAAVKALSEIPQSKILAVSSFYRTPPLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQ
QGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLFLNKLLHKKGFTKPDKW
>Mature_158_residues
TIAYIAIGSNLASPLEQVNAAVKALSEIPQSKILAVSSFYRTPPLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQ
GRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLFLNKLLHKKGFTKPDKW

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=159, Percent_Identity=88.6792452830189, Blast_Score=295, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6324073, Length=142, Percent_Identity=35.2112676056338, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 17997; Mature: 17866

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAYIAIGSNLASPLEQVNAAVKALSEIPQSKILAVSSFYRTPPLGPQDQPDYLNAAVA
CEEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHE
LETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMK
EECCCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEEEEECCCEECCCEEECCCCCCC
NRGFMLWPLFEIAPELVFPDGLFLNKLLHKKGFTKPDKW
CCCEEEEEHHHHCHHHCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TIAYIAIGSNLASPLEQVNAAVKALSEIPQSKILAVSSFYRTPPLGPQDQPDYLNAAVA
EEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHE
LETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMK
EECCCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEEEEECCCEECCCEEECCCCCCC
NRGFMLWPLFEIAPELVFPDGLFLNKLLHKKGFTKPDKW
CCCEEEEEHHHHCHHHCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]