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The map label for this gene is setA [H]

Identifier: 218547523

GI number: 218547523

Start: 91949

End: 93127

Strand: Direct

Name: setA [H]

Synonym: EFER_0085

Alternate gene names: 218547523

Gene position: 91949-93127 (Clockwise)

Preceding gene: 218547518

Following gene: 218547524

Centisome position: 2.0

GC content: 49.02

Gene sequence:

>1179_bases
ATGGTCTGGATTATGACGATGGCTCGCCGTATGAATGGTGTCTACGCGGCTTTTATGCTGGTCGCCTTTATGATGGGAGT
CGCAGGGGCGCTACAGGCTCCTACATTGAGTTTATTTCTCAGTCGTGAGGTTGGCGCCCAGCCATTCTGGGTCGGACTCT
TTTATACGGTGAATGCTATCGCCGGGATTGGCGTAAGTCTGTGGCTGGCAAAGCGTTCTGACAGCCAGGGCGACAGGCGT
AAACTGATCATGTTTTGCTGCCTGATGGCGATTGGCAATGCGCTACTGTTTGCATTTAATCGTCATTATCTGACATTGAT
CACCTGTGGTGTGTTTCTGGCCTCACTGGCTAATACAGCAATGCCACAGCTGTTTGCCCTGGCGCGGGAATATGCGGATA
ACTCTGCGCGTGAAGTCGTGATGTTTAGCTCAGTTATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGTCCACCGCTG
GCTTTTATGCTGGCGTTGAATTACGGCTTCACGGTGATGTTTTCGATTGCTGCCGGGATATTCGTCATCAGCCTGATATT
GATTGCGTTTATGCTTCCGTCTGTAACCCGGGTGGAGTTGCCGCCAGAAAATGCTCCAGCAATACAAGGTGGCTGGCAGG
ATAATAATGTTCGGATGTTGTTTGTTGCCTCAACGCTAATGTGGACCTGCAACACGATGTACATTATTGATATGCCATTG
TGGATAAGCAGCGATCTTGGATTACCTGACAAACTGGCGGGTCTACTGATGGGCACGGCAGCAGGGTTGGAAATCCCGGC
AATGATTCTGGCGGGTTACTTTGTAAAACATTTTGGCAAACGGCGCATGATGCTCGTGGCAGTAGCCGCTGGTGTGCTGT
TTTATATCGGGCTAATTTTCTTTCATAGTCGTATGGCGCTGATCACACTACAGCTGTTTAACGCAGTGTTTATCGGCATT
GTTGCAGGTATTGGGATGCTATGGTTTCAGGATTTGATGCCAGGCAGAGCGGGATCAGCTACAACATTATTTACTAATAG
CATTTCTACCGGGGTGATTCTGGCAGGTGTTATTCAGGGAGCAATAGCGCAAAGTTGGGGGCATTTTGCAGTCTATTGGG
CAATTGCTGCCATTTCGATCGTGACGTTATTTTTAACCGCGAAGGTAAAAGACATTTGA

Upstream 100 bases:

>100_bases
TTGCCGACATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATAATACCTGCTGGCTTTTTTTATTCTCG
CCGCGCTAAAAAGGAAACTT

Downstream 100 bases:

>100_bases
TGAATGTAGGATGAGTATAAAAAGGGGGAGAAAATGCTTCCCCCTTTTATCGTATACCGATTATTTCAGGACAATACGCT
TAATGTCGCCAATGATAAAG

Product: broad specificity sugar efflux system

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MVWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWVGLFYTVNAIAGIGVSLWLAKRSDSQGDRR
KLIMFCCLMAIGNALLFAFNRHYLTLITCGVFLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
AFMLALNYGFTVMFSIAAGIFVISLILIAFMLPSVTRVELPPENAPAIQGGWQDNNVRMLFVASTLMWTCNTMYIIDMPL
WISSDLGLPDKLAGLLMGTAAGLEIPAMILAGYFVKHFGKRRMMLVAVAAGVLFYIGLIFFHSRMALITLQLFNAVFIGI
VAGIGMLWFQDLMPGRAGSATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWAIAAISIVTLFLTAKVKDI

Sequences:

>Translated_392_residues
MVWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWVGLFYTVNAIAGIGVSLWLAKRSDSQGDRR
KLIMFCCLMAIGNALLFAFNRHYLTLITCGVFLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
AFMLALNYGFTVMFSIAAGIFVISLILIAFMLPSVTRVELPPENAPAIQGGWQDNNVRMLFVASTLMWTCNTMYIIDMPL
WISSDLGLPDKLAGLLMGTAAGLEIPAMILAGYFVKHFGKRRMMLVAVAAGVLFYIGLIFFHSRMALITLQLFNAVFIGI
VAGIGMLWFQDLMPGRAGSATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWAIAAISIVTLFLTAKVKDI
>Mature_392_residues
MVWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWVGLFYTVNAIAGIGVSLWLAKRSDSQGDRR
KLIMFCCLMAIGNALLFAFNRHYLTLITCGVFLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
AFMLALNYGFTVMFSIAAGIFVISLILIAFMLPSVTRVELPPENAPAIQGGWQDNNVRMLFVASTLMWTCNTMYIIDMPL
WISSDLGLPDKLAGLLMGTAAGLEIPAMILAGYFVKHFGKRRMMLVAVAAGVLFYIGLIFFHSRMALITLQLFNAVFIGI
VAGIGMLWFQDLMPGRAGSATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWAIAAISIVTLFLTAKVKDI

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport IPTG, lactose and glucose. Has broad substrate specificity, with preferences for glucosides or galactosides with alkyl or

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI48994875, Length=392, Percent_Identity=92.6020408163265, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI1788495, Length=385, Percent_Identity=52.987012987013, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1790091, Length=392, Percent_Identity=47.9591836734694, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42703; Mature: 42703

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWVGLFYTVNAI
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEHHCCCCCHHHHHHHHHHHH
AGIGVSLWLAKRSDSQGDRRKLIMFCCLMAIGNALLFAFNRHYLTLITCGVFLASLANTA
HHCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPLAFMLALNYGFTVMFSIAAGI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHCCHHHHHHHHHHH
FVISLILIAFMLPSVTRVELPPENAPAIQGGWQDNNVRMLFVASTLMWTCNTMYIIDMPL
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCEEEEEEECH
WISSDLGLPDKLAGLLMGTAAGLEIPAMILAGYFVKHFGKRRMMLVAVAAGVLFYIGLIF
HCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FHSRMALITLQLFNAVFIGIVAGIGMLWFQDLMPGRAGSATTLFTNSISTGVILAGVIQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHH
AIAQSWGHFAVYWAIAAISIVTLFLTAKVKDI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWVGLFYTVNAI
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEHHCCCCCHHHHHHHHHHHH
AGIGVSLWLAKRSDSQGDRRKLIMFCCLMAIGNALLFAFNRHYLTLITCGVFLASLANTA
HHCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPLAFMLALNYGFTVMFSIAAGI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHCCHHHHHHHHHHH
FVISLILIAFMLPSVTRVELPPENAPAIQGGWQDNNVRMLFVASTLMWTCNTMYIIDMPL
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCEEEEEEECH
WISSDLGLPDKLAGLLMGTAAGLEIPAMILAGYFVKHFGKRRMMLVAVAAGVLFYIGLIF
HCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FHSRMALITLQLFNAVFIGIVAGIGMLWFQDLMPGRAGSATTLFTNSISTGVILAGVIQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHH
AIAQSWGHFAVYWAIAAISIVTLFLTAKVKDI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 10209755; 10438463 [H]