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The map label for this gene is yfhM [H]

Identifier: 218532056

GI number: 218532056

Start: 4377170

End: 4378036

Strand: Reverse

Name: yfhM [H]

Synonym: Mchl_4150

Alternate gene names: 218532056

Gene position: 4378036-4377170 (Counterclockwise)

Preceding gene: 218532060

Following gene: 218532053

Centisome position: 75.77

GC content: 72.2

Gene sequence:

>867_bases
ATGTCCGATCCCGCCGTCAGCCACCGCCACATCGACCTCCGCGGTCTGCGCCTGCATGTCGCCGAGGCGGGGCCGGCGGA
CGGGGTGCCGACGATCATGCTGCACGGCTTTCCGGAATTCTGGTTCGGCTGGCGGCATCAGATCGGGCCGCTCGCGGGCA
GCGGCCTGCGCCTCCTCATCCCCGATCAGCGCGGCTACGGCCTGAGCGACCGCCCCAAGGGCATCGCCGCTTACCACCTC
GACCGGCTTGCCCAGGACGTGATCGCGCTCGCCGATGCCTGCGGGCTCGCGCGGTTCAACCTCGTCGGGCACGATTGGGG
CGGGCTCGTCGCGTTCTGGGTCGCGAGCTTCCACCCCGAGCGGGTCGAGCGGCTCGCCGCGCTGAACGCCTGCCATCCCG
GCGTGTTCGGCCCCTATCTCCGGCGCCATCCGGGGCAGGCCCTGCGCAGCGCCTATGCCGGCTTCTTCCAGCTCCCCCTG
CTGCCCGAACGGGTGCTGACGGCACGGGAGGGCCGCCTGCTGCGGGCCCTGATGCGCCGGTCGAGCGCGCCCGGCAGCTT
CACCGAGGCCGATCTCGACGTCTACGCGCGCGAATGGTTGCGCCCCGGCGCGGTCACCGCGATGCTGAACTGGTATCGGG
CGCTCGCTCAGCTGCCCCGCGAGCGGCACCCGCCGAAGATCGTCGCGCCGACGCTGATCCTGTGGGGGGAGCGCGATCAG
GCGCTCCAGGCGGGCCTGGCCGAGGCAAGCCTGGACCTATGTGAGCGGGGCCGCCTGCAGCGTTTTCCGCAGGCGACCCA
CTGGGTGCAGCACGACGCGCCGGAGGCGGTCAACGCCGCGCTGATCGACTTTCTGGGACGGTCGTAG

Upstream 100 bases:

>100_bases
CCGAACCGCGCCGGCCCTGCCGGGTTGGCCGAAATACTGGCAACTGTCCTGGCATCTGTCCCGACTTGTCCATCGACCGC
GCGCGAGTGTCCCCGAACCC

Downstream 100 bases:

>100_bases
ACAACCATAAAAGAATGCCGGGGCAGAGCCCCGGCATTCCGCCAAAGGGATGATCCCTTTGGAGTTCCGCTGATCAGCGG
CCGCTCATGCCGGCGCCGCC

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSDPAVSHRHIDLRGLRLHVAEAGPADGVPTIMLHGFPEFWFGWRHQIGPLAGSGLRLLIPDQRGYGLSDRPKGIAAYHL
DRLAQDVIALADACGLARFNLVGHDWGGLVAFWVASFHPERVERLAALNACHPGVFGPYLRRHPGQALRSAYAGFFQLPL
LPERVLTAREGRLLRALMRRSSAPGSFTEADLDVYAREWLRPGAVTAMLNWYRALAQLPRERHPPKIVAPTLILWGERDQ
ALQAGLAEASLDLCERGRLQRFPQATHWVQHDAPEAVNAALIDFLGRS

Sequences:

>Translated_288_residues
MSDPAVSHRHIDLRGLRLHVAEAGPADGVPTIMLHGFPEFWFGWRHQIGPLAGSGLRLLIPDQRGYGLSDRPKGIAAYHL
DRLAQDVIALADACGLARFNLVGHDWGGLVAFWVASFHPERVERLAALNACHPGVFGPYLRRHPGQALRSAYAGFFQLPL
LPERVLTAREGRLLRALMRRSSAPGSFTEADLDVYAREWLRPGAVTAMLNWYRALAQLPRERHPPKIVAPTLILWGERDQ
ALQAGLAEASLDLCERGRLQRFPQATHWVQHDAPEAVNAALIDFLGRS
>Mature_287_residues
SDPAVSHRHIDLRGLRLHVAEAGPADGVPTIMLHGFPEFWFGWRHQIGPLAGSGLRLLIPDQRGYGLSDRPKGIAAYHLD
RLAQDVIALADACGLARFNLVGHDWGGLVAFWVASFHPERVERLAALNACHPGVFGPYLRRHPGQALRSAYAGFFQLPLL
PERVLTAREGRLLRALMRRSSAPGSFTEADLDVYAREWLRPGAVTAMLNWYRALAQLPRERHPPKIVAPTLILWGERDQA
LQAGLAEASLDLCERGRLQRFPQATHWVQHDAPEAVNAALIDFLGRS

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=295, Percent_Identity=36.271186440678, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI218777840, Length=292, Percent_Identity=35.2739726027397, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI13376164, Length=292, Percent_Identity=35.2739726027397, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI27597073, Length=321, Percent_Identity=30.2180685358255, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17562298, Length=287, Percent_Identity=35.5400696864112, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17554012, Length=284, Percent_Identity=33.8028169014084, Blast_Score=182, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 31974; Mature: 31843

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPAVSHRHIDLRGLRLHVAEAGPADGVPTIMLHGFPEFWFGWRHQIGPLAGSGLRLLI
CCCCCCCCCEEEEEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCEEEE
PDQRGYGLSDRPKGIAAYHLDRLAQDVIALADACGLARFNLVGHDWGGLVAFWVASFHPE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCHH
RVERLAALNACHPGVFGPYLRRHPGQALRSAYAGFFQLPLLPERVLTAREGRLLRALMRR
HHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
SSAPGSFTEADLDVYAREWLRPGAVTAMLNWYRALAQLPRERHPPKIVAPTLILWGERDQ
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEECCCHH
ALQAGLAEASLDLCERGRLQRFPQATHWVQHDAPEAVNAALIDFLGRS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDPAVSHRHIDLRGLRLHVAEAGPADGVPTIMLHGFPEFWFGWRHQIGPLAGSGLRLLI
CCCCCCCCEEEEEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCEEEE
PDQRGYGLSDRPKGIAAYHLDRLAQDVIALADACGLARFNLVGHDWGGLVAFWVASFHPE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCHH
RVERLAALNACHPGVFGPYLRRHPGQALRSAYAGFFQLPLLPERVLTAREGRLLRALMRR
HHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
SSAPGSFTEADLDVYAREWLRPGAVTAMLNWYRALAQLPRERHPPKIVAPTLILWGERDQ
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEECCCHH
ALQAGLAEASLDLCERGRLQRFPQATHWVQHDAPEAVNAALIDFLGRS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]