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The map label for this gene is nusB [H]

Identifier: 218531417

GI number: 218531417

Start: 3670689

End: 3671204

Strand: Reverse

Name: nusB [H]

Synonym: Mchl_3485

Alternate gene names: 218531417

Gene position: 3671204-3670689 (Counterclockwise)

Preceding gene: 218531418

Following gene: 218531416

Centisome position: 63.54

GC content: 70.35

Gene sequence:

>516_bases
ATGACAGACCCCGCCGCGACACCGGCCCAACCGAAGCCGAATGCCCGCACCGGTGCCCGCCTCGCCGTGGTGCAGGCGCT
CTACGAGATGGAGATCTCGGACAAGGGCGTGATCGAGGCGCTCGCCGAGTTCGAGGCCTTCTGGATCGGTCAGGAGGTCG
ATGGCGTCGTCCACCCGCCGGCCGAGATCGCGTTCTTCCGCGACGTGCTCCGCGGTGCCGTGGAGGAACAGCGCGCCATC
GACCCGAAGCTCGATACGGCGTTGGCAAAGGGCTGGCCGCTGCGCCGGCTGGAAGCGGTGCTGCGCGCCATCCTGCGGGC
GGGCGCCTATGAGCTGATGTTCCGCCGCGACGTGCCGGCCCGCGCCGCGATCTCGCAATATGTCGAGGTCGCCTACAGCT
TCTATGGCGGCGACGAGCCGGGCATGGTCAACGCCGTGCTCGACCGGGTCGGCCGCGAGGTGCGCTCCACCGAGTTCGGC
ATCCCGGCCGCTCCCGGCCCGGCGAAGAAGGGCTGA

Upstream 100 bases:

>100_bases
CTCGCGCCCGGGTCTCCGACATGAACAAGGGCGGCGGTGCAGCGGAGGCGGCGCTGAGCCTCCTCGCCATCAAGCGCGCC
GCCAACCTGGAACCCGCCCG

Downstream 100 bases:

>100_bases
CCGGAATGGCGCGCGCGGGCGAGGAGGCGCTGATCGCGCGCTACTTCGCGCCGCTCAGCGGCCCCGGCGCCGAGGGCCTG
CGCGATGACGCCGCGAGCCT

Product: NusB antitermination factor

Products: NA

Alternate protein names: Protein nusB [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MTDPAATPAQPKPNARTGARLAVVQALYEMEISDKGVIEALAEFEAFWIGQEVDGVVHPPAEIAFFRDVLRGAVEEQRAI
DPKLDTALAKGWPLRRLEAVLRAILRAGAYELMFRRDVPARAAISQYVEVAYSFYGGDEPGMVNAVLDRVGREVRSTEFG
IPAAPGPAKKG

Sequences:

>Translated_171_residues
MTDPAATPAQPKPNARTGARLAVVQALYEMEISDKGVIEALAEFEAFWIGQEVDGVVHPPAEIAFFRDVLRGAVEEQRAI
DPKLDTALAKGWPLRRLEAVLRAILRAGAYELMFRRDVPARAAISQYVEVAYSFYGGDEPGMVNAVLDRVGREVRSTEFG
IPAAPGPAKKG
>Mature_170_residues
TDPAATPAQPKPNARTGARLAVVQALYEMEISDKGVIEALAEFEAFWIGQEVDGVVHPPAEIAFFRDVLRGAVEEQRAID
PKLDTALAKGWPLRRLEAVLRAILRAGAYELMFRRDVPARAAISQYVEVAYSFYGGDEPGMVNAVLDRVGREVRSTEFGI
PAAPGPAKKG

Specific function: Involved in the transcription termination process [H]

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family [H]

Homologues:

Organism=Escherichia coli, GI1786618, Length=144, Percent_Identity=34.0277777777778, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011605
- InterPro:   IPR006027 [H]

Pfam domain/function: PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 18606; Mature: 18475

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPAATPAQPKPNARTGARLAVVQALYEMEISDKGVIEALAEFEAFWIGQEVDGVVHPP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
AEIAFFRDVLRGAVEEQRAIDPKLDTALAKGWPLRRLEAVLRAILRAGAYELMFRRDVPA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCH
RAAISQYVEVAYSFYGGDEPGMVNAVLDRVGREVRSTEFGIPAAPGPAKKG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDPAATPAQPKPNARTGARLAVVQALYEMEISDKGVIEALAEFEAFWIGQEVDGVVHPP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
AEIAFFRDVLRGAVEEQRAIDPKLDTALAKGWPLRRLEAVLRAILRAGAYELMFRRDVPA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCH
RAAISQYVEVAYSFYGGDEPGMVNAVLDRVGREVRSTEFGIPAAPGPAKKG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]