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The map label for this gene is ribH

Identifier: 218531418

GI number: 218531418

Start: 3671201

End: 3671722

Strand: Reverse

Name: ribH

Synonym: Mchl_3486

Alternate gene names: 218531418

Gene position: 3671722-3671201 (Counterclockwise)

Preceding gene: 218531421

Following gene: 218531417

Centisome position: 63.55

GC content: 71.84

Gene sequence:

>522_bases
ATGGTCTCCCAACGCCGGGACGCGGACGCGCCCAAGGACAGCCCTAAAAGCATCCTCGAAAGCCTCGCCGGCACCCGCGT
GCTCGTGGTCGAGGCGCGCTACTACGACGACATCGCCGACGAATTGCTGGCGGGCGCCCGCGCGGCGATCGAAGCGGTCG
GCGCCGAGGCGCGGGTCTTCACGGTGCCGGGCGCCCTCGAGATCCCCGCCGCCATCGCGATCCTGATGGATGCCGGCCGC
AAGGCCGGCGGCCCCTACGATGCGGCGGTGGCGCTCGGCTGCGTCATCCGCGGCGAGACCGGCCATTACGACATCGTCGC
CGGCGAGAGCGCCCGCGCGCTGATGGACCTTTCCGTGGCCGAGCACCTGCCGCTCGGCAACGGCATCCTCACCGTGGAGA
CCATGGAGCAGGCGCTCGCTCGCGCCCGGGTCTCCGACATGAACAAGGGCGGCGGTGCAGCGGAGGCGGCGCTGAGCCTC
CTCGCCATCAAGCGCGCCGCCAACCTGGAACCCGCCCGATGA

Upstream 100 bases:

>100_bases
CACCCGCCGCGCCCCGTGCCGCTTGTGGAGCCCGCGGCACTTGGGCTAACCCTCCGGCCCGCCCCGAACATTCGTGAACT
CGCCCCGGAACGACAGTCGC

Downstream 100 bases:

>100_bases
CAGACCCCGCCGCGACACCGGCCCAACCGAAGCCGAATGCCCGCACCGGTGCCCGCCTCGCCGTGGTGCAGGCGCTCTAC
GAGATGGAGATCTCGGACAA

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MVSQRRDADAPKDSPKSILESLAGTRVLVVEARYYDDIADELLAGARAAIEAVGAEARVFTVPGALEIPAAIAILMDAGR
KAGGPYDAAVALGCVIRGETGHYDIVAGESARALMDLSVAEHLPLGNGILTVETMEQALARARVSDMNKGGGAAEAALSL
LAIKRAANLEPAR

Sequences:

>Translated_173_residues
MVSQRRDADAPKDSPKSILESLAGTRVLVVEARYYDDIADELLAGARAAIEAVGAEARVFTVPGALEIPAAIAILMDAGR
KAGGPYDAAVALGCVIRGETGHYDIVAGESARALMDLSVAEHLPLGNGILTVETMEQALARARVSDMNKGGGAAEAALSL
LAIKRAANLEPAR
>Mature_173_residues
MVSQRRDADAPKDSPKSILESLAGTRVLVVEARYYDDIADELLAGARAAIEAVGAEARVFTVPGALEIPAAIAILMDAGR
KAGGPYDAAVALGCVIRGETGHYDIVAGESARALMDLSVAEHLPLGNGILTVETMEQALARARVSDMNKGGGAAEAALSL
LAIKRAANLEPAR

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=145, Percent_Identity=38.6206896551724, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324429, Length=149, Percent_Identity=33.5570469798658, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_METC4 (B7KUS7)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002422234.1
- ProteinModelPortal:   B7KUS7
- SMR:   B7KUS7
- GeneID:   7114244
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_3486
- HOGENOM:   HBG311126
- OMA:   SSRALMD
- ProtClustDB:   CLSK2331551
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 17877; Mature: 17877

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSQRRDADAPKDSPKSILESLAGTRVLVVEARYYDDIADELLAGARAAIEAVGAEARVF
CCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TVPGALEIPAAIAILMDAGRKAGGPYDAAVALGCVIRGETGHYDIVAGESARALMDLSVA
ECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHH
EHLPLGNGILTVETMEQALARARVSDMNKGGGAAEAALSLLAIKRAANLEPAR
HHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVSQRRDADAPKDSPKSILESLAGTRVLVVEARYYDDIADELLAGARAAIEAVGAEARVF
CCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TVPGALEIPAAIAILMDAGRKAGGPYDAAVALGCVIRGETGHYDIVAGESARALMDLSVA
ECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHH
EHLPLGNGILTVETMEQALARARVSDMNKGGGAAEAALSLLAIKRAANLEPAR
HHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA