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The map label for this gene is 218236043

Identifier: 218236043

GI number: 218236043

Start: 2479801

End: 2481234

Strand: Reverse

Name: 218236043

Synonym: BCB4264_A2558

Alternate gene names: NA

Gene position: 2481234-2479801 (Counterclockwise)

Preceding gene: 218234801

Following gene: 218233075

Centisome position: 45.79

GC content: 33.82

Gene sequence:

>1434_bases
ATGCAAAATTTGAAAGATTACTATATAAAGAGCTTACAAGATGGTCGTAACGTTTGGCTCTATGGTCAAAAAGTAGATGT
AACTACAGACAAACATTTTGCAGGAACATTAAGTACCATTTCTAATTTATTTACATTGTTTGATGATCCAATACGAAGAG
ATTCTGTTGGATATGTTAGTCCAAAAACGAAAGAATTTGTTCATACAGCCTTTTTAGTTCCGAATTCCTATAACGAATTA
TTAATGAGGAGAACCGCTTTTGAAACGTGGAGTTATGCTACAGACGGTGTAATGAGTAGACTATCTGATTACGCAAGATC
GAGACTTACTGGCTGGTACGTATCAAAATATTTTTATAAAAAATTCGATGCACAGTTTCCAAATAAAATTACATCTTATT
ATGAAGAAGCGAGAGATAATCACTTATTCTTAAGCGTAGTTCAACGTGATCCACAAATAAATCGATCTACAGAATCAATG
TTGAATACAAACGACTTAGGATTACTAAGAGTTACGAAAAAAACTTCTGATGGAATTTATTTAAGTGGTGCTAAAATGAT
TGGTACTGCTTCTCCTTATTCAAATGATTTATTAATCTATCCATTAGCAAAACTGACTGATACTCAAAAAGATTTAGCTC
ACTTTCTCATTGTGGCAGCCAATTCCCCTGGATTACATATGGTTTGTAGAGAACCATTTGCGAATACACATTCAATTACA
GTTGATTCCCCTCTTAGCTCACAGTATGATGAAATGGATGCACTATTAATCTTTGATAATGTATTTGTACCATGGGAACG
AGTCTTACTATATAACAACCCTGAAGCGTTATGGGCAATAAAGTCAGATGTAGCTTCTAGTAGTTTAGCTTATCACCAAG
CAATTGTACGATTAGTAGTAAAGCTAGAATTTATAACTGCAATTGCTTGTGAAATTGCTGAAGCGATCGGTGCTACTACT
TATTTGCATGTTCAAGAGAAGCTTGGGGAATTAATTATGCAAATCGAAACAATTCGCGCACTTTTAATTGCTGCAGAAGT
TGAAGGTACTACAAATGAAACGAAAACTTATTTGCCGAATTTTAAATATATCGAAACTGCAAGGAATCTTGGTTCCAAAT
ATTATCCCCGTGCAATAGAAATTTTACAATTAATTGGAGCAGGTGGGTTCATTCAACTTCCTTCGAGCTCGAATGACTTC
CAAAGTCCGATAGCTGATTTATTGAAAAAGTACTTTAAAGGAACGAATATAGATGCCGAACAACGCACAAAATTATTTAA
GTTGGCCTGGGATATCATTGGAAGTCCATTAGGATCTAGACACGAATTGTATGAAAGATTTTATGCCGGAGATCCAATAC
GAAATACAGCTACGCATTATGTAAATTATGATAAAAATCATTTTAAAAAGATGCTAAGTAAATATTTACATTAA

Upstream 100 bases:

>100_bases
AATGTATAATGCTACAGATTCAGCAGTAATAACTTTCCACGTATATTCCCCACCATTAAAAGATGTCCAAATATATGAAT
AGTCTTTAGAAGGAGGAAAT

Downstream 100 bases:

>100_bases
TGAAAAAGAGCACTCTTTTCTAAGAGCGCTCTTAAAAAACATATAGTTATTCAGTTACATATATAGTTGCTCCGTCCACA
ACTGCTACTTTTCTCTGTAA

Product: 4-hydroxyphenylacetate 3-hydroxylase family

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MQNLKDYYIKSLQDGRNVWLYGQKVDVTTDKHFAGTLSTISNLFTLFDDPIRRDSVGYVSPKTKEFVHTAFLVPNSYNEL
LMRRTAFETWSYATDGVMSRLSDYARSRLTGWYVSKYFYKKFDAQFPNKITSYYEEARDNHLFLSVVQRDPQINRSTESM
LNTNDLGLLRVTKKTSDGIYLSGAKMIGTASPYSNDLLIYPLAKLTDTQKDLAHFLIVAANSPGLHMVCREPFANTHSIT
VDSPLSSQYDEMDALLIFDNVFVPWERVLLYNNPEALWAIKSDVASSSLAYHQAIVRLVVKLEFITAIACEIAEAIGATT
YLHVQEKLGELIMQIETIRALLIAAEVEGTTNETKTYLPNFKYIETARNLGSKYYPRAIEILQLIGAGGFIQLPSSSNDF
QSPIADLLKKYFKGTNIDAEQRTKLFKLAWDIIGSPLGSRHELYERFYAGDPIRNTATHYVNYDKNHFKKMLSKYLH

Sequences:

>Translated_477_residues
MQNLKDYYIKSLQDGRNVWLYGQKVDVTTDKHFAGTLSTISNLFTLFDDPIRRDSVGYVSPKTKEFVHTAFLVPNSYNEL
LMRRTAFETWSYATDGVMSRLSDYARSRLTGWYVSKYFYKKFDAQFPNKITSYYEEARDNHLFLSVVQRDPQINRSTESM
LNTNDLGLLRVTKKTSDGIYLSGAKMIGTASPYSNDLLIYPLAKLTDTQKDLAHFLIVAANSPGLHMVCREPFANTHSIT
VDSPLSSQYDEMDALLIFDNVFVPWERVLLYNNPEALWAIKSDVASSSLAYHQAIVRLVVKLEFITAIACEIAEAIGATT
YLHVQEKLGELIMQIETIRALLIAAEVEGTTNETKTYLPNFKYIETARNLGSKYYPRAIEILQLIGAGGFIQLPSSSNDF
QSPIADLLKKYFKGTNIDAEQRTKLFKLAWDIIGSPLGSRHELYERFYAGDPIRNTATHYVNYDKNHFKKMLSKYLH
>Mature_477_residues
MQNLKDYYIKSLQDGRNVWLYGQKVDVTTDKHFAGTLSTISNLFTLFDDPIRRDSVGYVSPKTKEFVHTAFLVPNSYNEL
LMRRTAFETWSYATDGVMSRLSDYARSRLTGWYVSKYFYKKFDAQFPNKITSYYEEARDNHLFLSVVQRDPQINRSTESM
LNTNDLGLLRVTKKTSDGIYLSGAKMIGTASPYSNDLLIYPLAKLTDTQKDLAHFLIVAANSPGLHMVCREPFANTHSIT
VDSPLSSQYDEMDALLIFDNVFVPWERVLLYNNPEALWAIKSDVASSSLAYHQAIVRLVVKLEFITAIACEIAEAIGATT
YLHVQEKLGELIMQIETIRALLIAAEVEGTTNETKTYLPNFKYIETARNLGSKYYPRAIEILQLIGAGGFIQLPSSSNDF
QSPIADLLKKYFKGTNIDAEQRTKLFKLAWDIIGSPLGSRHELYERFYAGDPIRNTATHYVNYDKNHFKKMLSKYLH

Specific function: Utilizes FADH(2) supplied by hpaC, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 54407; Mature: 54407

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLKDYYIKSLQDGRNVWLYGQKVDVTTDKHFAGTLSTISNLFTLFDDPIRRDSVGYVS
CCCHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PKTKEFVHTAFLVPNSYNELLMRRTAFETWSYATDGVMSRLSDYARSRLTGWYVSKYFYK
CCHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFDAQFPNKITSYYEEARDNHLFLSVVQRDPQINRSTESMLNTNDLGLLRVTKKTSDGIY
HHCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHCCCCCCEEEEEEEECCCCEE
LSGAKMIGTASPYSNDLLIYPLAKLTDTQKDLAHFLIVAANSPGLHMVCREPFANTHSIT
EECCEEEECCCCCCCCEEEEEHHHHCCCHHHHEEEEEEEECCCCEEEEECCCCCCCCEEE
VDSPLSSQYDEMDALLIFDNVFVPWERVLLYNNPEALWAIKSDVASSSLAYHQAIVRLVV
ECCCCCCCHHHCCEEEEECCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHH
KLEFITAIACEIAEAIGATTYLHVQEKLGELIMQIETIRALLIAAEVEGTTNETKTYLPN
HHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
FKYIETARNLGSKYYPRAIEILQLIGAGGFIQLPSSSNDFQSPIADLLKKYFKGTNIDAE
CHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCHH
QRTKLFKLAWDIIGSPLGSRHELYERFYAGDPIRNTATHYVNYDKNHFKKMLSKYLH
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQNLKDYYIKSLQDGRNVWLYGQKVDVTTDKHFAGTLSTISNLFTLFDDPIRRDSVGYVS
CCCHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PKTKEFVHTAFLVPNSYNELLMRRTAFETWSYATDGVMSRLSDYARSRLTGWYVSKYFYK
CCHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFDAQFPNKITSYYEEARDNHLFLSVVQRDPQINRSTESMLNTNDLGLLRVTKKTSDGIY
HHCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHCCCCCCEEEEEEEECCCCEE
LSGAKMIGTASPYSNDLLIYPLAKLTDTQKDLAHFLIVAANSPGLHMVCREPFANTHSIT
EECCEEEECCCCCCCCEEEEEHHHHCCCHHHHEEEEEEEECCCCEEEEECCCCCCCCEEE
VDSPLSSQYDEMDALLIFDNVFVPWERVLLYNNPEALWAIKSDVASSSLAYHQAIVRLVV
ECCCCCCCHHHCCEEEEECCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHH
KLEFITAIACEIAEAIGATTYLHVQEKLGELIMQIETIRALLIAAEVEGTTNETKTYLPN
HHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
FKYIETARNLGSKYYPRAIEILQLIGAGGFIQLPSSSNDFQSPIADLLKKYFKGTNIDAE
CHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCHH
QRTKLFKLAWDIIGSPLGSRHELYERFYAGDPIRNTATHYVNYDKNHFKKMLSKYLH
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA