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The map label for this gene is pepF [H]

Identifier: 218233075

GI number: 218233075

Start: 2475433

End: 2477124

Strand: Reverse

Name: pepF [H]

Synonym: BCB4264_A2554

Alternate gene names: 218233075

Gene position: 2477124-2475433 (Counterclockwise)

Preceding gene: 218236043

Following gene: 218231677

Centisome position: 45.71

GC content: 30.56

Gene sequence:

>1692_bases
ATGCAACGTGTAAATACGATTGATATTAGTAATGTACTACAATTGGAAAAAACTCTTTCGACTTTATTAAATAAAATGAT
TTCTTCAAAACTAGACCTTGAAAATTGGTTAAAAGAGCAGTCAAAAGTAATTTGGGACATCGAAGAACAATTAAGATCAC
ATTACATCGCCTTTCAATGTAATACGGATGATGAAGAAATAAAGGATACTTTTGAACATGATCAACAATTTGTAAAGCCA
CTTTTAAAGCGGTATCAAAATTTATTGGATAATAAATATTTAGAGTCACCTTTTCGAATGGAATTAGATTCTAACGTATA
TGGTTTATTAGATACAAAAATAAAAAATGCTCAAAAGTTATTTTGTGAGGAAAATATCGAATTAGAAATAAAGGAAGACA
AATTAATCACAGAGTACTTTGAAATCACTGGAGGATTAAGTGGAATTTGGGACGAAGAAGAAAAAACGATAACAGAATTA
CAATCGTATTTACAAGATTCGAATCGTGACACCCGTAAAAAGGCAAAAACCATTATTTCTGAACAATTTTTATCTGTTGA
AAAAGAACTCCAAAATATATTAAATCAATTAATTGAAATTCGCCATCAAAAAGCAAAAAATATTCAACTAGAGAATTATC
GCGATTATATGTTCAAAAAATACGAGCGTTTTGATTATTCAGCGAAAGATTGCTATGAACTAGCTGAATCCATACGTAAG
TATGTTGTGCCTCTTAAAGATAAAATATTACTAGAGAAAAAAGAGAAACTGCAAGTGGATACGCTTCGCCCGTGGGATGT
ATCAGCTGTAACTCCAGATCAAAAAGTACTTAAACCAATTGCAAATGAAAATGATTTAATTGAAAAGAGCACTCATATCT
TTAATAAACTAGATGTTGAATTTTCAGCACTTTTAAACCGAATGTATAAACACAATTGTCTTGATTTAACAAGCCGCAAA
GGAAAAGCAGCTGGTGGCTTTTGTGAGTACTTACCAGCTTCACAATTATCTTATATTTTTATGAACTTAAATTATACACA
AGATGATATTGTCACTTTTATACATGAAATGGGGCATAGCATTCATAACGAACTCATAAAACCATTAAAATTACGACAGT
ATATAGAAATTCCAGCTGAAACAGCTGAGCTAGCCAGTATGACAATGGAGTTATTCTCGCTTAATTATTGGGATACCTTC
TATACAGACAAAAAAGATTTAAAACAAGCAAAAATTAACTTCTTTAAAGATGTTATTTCATATTTACCTATTATGCTTAT
TGTCGATCAATTTCAACACTGGCTGTATGAAAATCCAAGTCATACTTCTGAAGAAAGAAATGAAAAATACTTACAACTTC
AAAAACATTATCAGTCTAGCGTCATACATATTGATGGCTACGAAAACTGGATAGCAACGAGTTGGTTACCTGTACTCCAT
ATTTTTGAAGTACCATTTTATTATATTGAATATGCGATCGCCCAACTTGGTGCATTACAAATGTATAAACAATATAAAGA
GGATCCTAAGCAAGCACTAGAAAATTATAAAAAGGCATTATCACTAGGAAGCTCGCAATCTATAAAAGAAGTGTATGATG
CAGCCGGTATTCGTTTTGATTTTTCTGGTGAAACAATTAAAGAATTAATGCTATTTGTAGAAAAAGAATTAGAATTACTT
GAGCAATTATAA

Upstream 100 bases:

>100_bases
ATTAAATTTAACATTATTTCACATTTCCCCCACTTAACAAGGAAATTACAATTAATAAATAGAATATCTCTATGAATAAA
TAAAAAGTGAGGGATATATA

Downstream 100 bases:

>100_bases
GAAGAAAGCTGCACCTATATTTTTGGTGTAGTTTTCTTCTTTATTTTATTAATCAGTTCTATGTACTGAGAATCTCCATA
TCCGATTGTAGGAAAAAGAT

Product: hypothetical protein

Products: hydrolyzed oligopeptide [C]

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MQRVNTIDISNVLQLEKTLSTLLNKMISSKLDLENWLKEQSKVIWDIEEQLRSHYIAFQCNTDDEEIKDTFEHDQQFVKP
LLKRYQNLLDNKYLESPFRMELDSNVYGLLDTKIKNAQKLFCEENIELEIKEDKLITEYFEITGGLSGIWDEEEKTITEL
QSYLQDSNRDTRKKAKTIISEQFLSVEKELQNILNQLIEIRHQKAKNIQLENYRDYMFKKYERFDYSAKDCYELAESIRK
YVVPLKDKILLEKKEKLQVDTLRPWDVSAVTPDQKVLKPIANENDLIEKSTHIFNKLDVEFSALLNRMYKHNCLDLTSRK
GKAAGGFCEYLPASQLSYIFMNLNYTQDDIVTFIHEMGHSIHNELIKPLKLRQYIEIPAETAELASMTMELFSLNYWDTF
YTDKKDLKQAKINFFKDVISYLPIMLIVDQFQHWLYENPSHTSEERNEKYLQLQKHYQSSVIHIDGYENWIATSWLPVLH
IFEVPFYYIEYAIAQLGALQMYKQYKEDPKQALENYKKALSLGSSQSIKEVYDAAGIRFDFSGETIKELMLFVEKELELL
EQL

Sequences:

>Translated_563_residues
MQRVNTIDISNVLQLEKTLSTLLNKMISSKLDLENWLKEQSKVIWDIEEQLRSHYIAFQCNTDDEEIKDTFEHDQQFVKP
LLKRYQNLLDNKYLESPFRMELDSNVYGLLDTKIKNAQKLFCEENIELEIKEDKLITEYFEITGGLSGIWDEEEKTITEL
QSYLQDSNRDTRKKAKTIISEQFLSVEKELQNILNQLIEIRHQKAKNIQLENYRDYMFKKYERFDYSAKDCYELAESIRK
YVVPLKDKILLEKKEKLQVDTLRPWDVSAVTPDQKVLKPIANENDLIEKSTHIFNKLDVEFSALLNRMYKHNCLDLTSRK
GKAAGGFCEYLPASQLSYIFMNLNYTQDDIVTFIHEMGHSIHNELIKPLKLRQYIEIPAETAELASMTMELFSLNYWDTF
YTDKKDLKQAKINFFKDVISYLPIMLIVDQFQHWLYENPSHTSEERNEKYLQLQKHYQSSVIHIDGYENWIATSWLPVLH
IFEVPFYYIEYAIAQLGALQMYKQYKEDPKQALENYKKALSLGSSQSIKEVYDAAGIRFDFSGETIKELMLFVEKELELL
EQL
>Mature_563_residues
MQRVNTIDISNVLQLEKTLSTLLNKMISSKLDLENWLKEQSKVIWDIEEQLRSHYIAFQCNTDDEEIKDTFEHDQQFVKP
LLKRYQNLLDNKYLESPFRMELDSNVYGLLDTKIKNAQKLFCEENIELEIKEDKLITEYFEITGGLSGIWDEEEKTITEL
QSYLQDSNRDTRKKAKTIISEQFLSVEKELQNILNQLIEIRHQKAKNIQLENYRDYMFKKYERFDYSAKDCYELAESIRK
YVVPLKDKILLEKKEKLQVDTLRPWDVSAVTPDQKVLKPIANENDLIEKSTHIFNKLDVEFSALLNRMYKHNCLDLTSRK
GKAAGGFCEYLPASQLSYIFMNLNYTQDDIVTFIHEMGHSIHNELIKPLKLRQYIEIPAETAELASMTMELFSLNYWDTF
YTDKKDLKQAKINFFKDVISYLPIMLIVDQFQHWLYENPSHTSEERNEKYLQLQKHYQSSVIHIDGYENWIATSWLPVLH
IFEVPFYYIEYAIAQLGALQMYKQYKEDPKQALENYKKALSLGSSQSIKEVYDAAGIRFDFSGETIKELMLFVEKELELL
EQL

Specific function: May Play A Specific Role In The Degradation Of Signal Peptides After They Are Released From Precursor Forms Of Secreted Proteins. Can Cleave N-Acetyl-L-Ala(4). [C]

COG id: COG1164

COG function: function code E; Oligoendopeptidase F

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M3B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001567
- InterPro:   IPR004438
- InterPro:   IPR013647 [H]

Pfam domain/function: PF01432 Peptidase_M3; PF08439 Peptidase_M3_N [H]

EC number: 3.4.24.70 [C]

Molecular weight: Translated: 66682; Mature: 66682

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRVNTIDISNVLQLEKTLSTLLNKMISSKLDLENWLKEQSKVIWDIEEQLRSHYIAFQC
CCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEE
NTDDEEIKDTFEHDQQFVKPLLKRYQNLLDNKYLESPFRMELDSNVYGLLDTKIKNAQKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
FCEENIELEIKEDKLITEYFEITGGLSGIWDEEEKTITELQSYLQDSNRDTRKKAKTIIS
HHCCCCCEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EQFLSVEKELQNILNQLIEIRHQKAKNIQLENYRDYMFKKYERFDYSAKDCYELAESIRK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
YVVPLKDKILLEKKEKLQVDTLRPWDVSAVTPDQKVLKPIANENDLIEKSTHIFNKLDVE
HHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHH
FSALLNRMYKHNCLDLTSRKGKAAGGFCEYLPASQLSYIFMNLNYTQDDIVTFIHEMGHS
HHHHHHHHHHCCHHHHHCCCCCCCCCHHHHCCHHHHEEEEEECCCCHHHHHHHHHHHHHH
IHNELIKPLKLRQYIEIPAETAELASMTMELFSLNYWDTFYTDKKDLKQAKINFFKDVIS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHH
YLPIMLIVDQFQHWLYENPSHTSEERNEKYLQLQKHYQSSVIHIDGYENWIATSWLPVLH
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHH
IFEVPFYYIEYAIAQLGALQMYKQYKEDPKQALENYKKALSLGSSQSIKEVYDAAGIRFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEE
FSGETIKELMLFVEKELELLEQL
CCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQRVNTIDISNVLQLEKTLSTLLNKMISSKLDLENWLKEQSKVIWDIEEQLRSHYIAFQC
CCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEE
NTDDEEIKDTFEHDQQFVKPLLKRYQNLLDNKYLESPFRMELDSNVYGLLDTKIKNAQKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
FCEENIELEIKEDKLITEYFEITGGLSGIWDEEEKTITELQSYLQDSNRDTRKKAKTIIS
HHCCCCCEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EQFLSVEKELQNILNQLIEIRHQKAKNIQLENYRDYMFKKYERFDYSAKDCYELAESIRK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
YVVPLKDKILLEKKEKLQVDTLRPWDVSAVTPDQKVLKPIANENDLIEKSTHIFNKLDVE
HHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHH
FSALLNRMYKHNCLDLTSRKGKAAGGFCEYLPASQLSYIFMNLNYTQDDIVTFIHEMGHS
HHHHHHHHHHCCHHHHHCCCCCCCCCHHHHCCHHHHEEEEEECCCCHHHHHHHHHHHHHH
IHNELIKPLKLRQYIEIPAETAELASMTMELFSLNYWDTFYTDKKDLKQAKINFFKDVIS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHH
YLPIMLIVDQFQHWLYENPSHTSEERNEKYLQLQKHYQSSVIHIDGYENWIATSWLPVLH
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHH
IFEVPFYYIEYAIAQLGALQMYKQYKEDPKQALENYKKALSLGSSQSIKEVYDAAGIRFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEE
FSGETIKELMLFVEKELELLEQL
CCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Co2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oligopeptide; H2O [C]

Specific reaction: Oligopeptide + H2O = Hydrolyzed oligopeptide [C]

General reaction: Hydrolysis of peptide bond [C]

Inhibitor: Cu2+; EDTA; Ni2+; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]