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The map label for this gene is appA [H]

Identifier: 218233727

GI number: 218233727

Start: 917086

End: 918672

Strand: Direct

Name: appA [H]

Synonym: BCB4264_A0945

Alternate gene names: 218233727

Gene position: 917086-918672 (Clockwise)

Preceding gene: 218234462

Following gene: 218234318

Centisome position: 16.92

GC content: 35.66

Gene sequence:

>1587_bases
ATGAAGAAGAAGTTGTTTGTATTACCATTTGTCCTACTATTATTTATTGCATTAGCTGCTTGTTCAGGGGAAAAAGATTC
GAAGCAAGCGAGCACTAGTAAATCAGGGACTCCGAAAGACGGAGGGGCGTTAACGATTGGTGTTAGCGATAATCCAGATA
CGATGAATCCGCTTTATGCGAATGATCGTGTGTCATTAACTGTGCAGCAAGCTTTATATGCGCCGCTATATCATATGGAA
GACGGTAAGAAAAAGTTTGTTCTTGCTGAGAGCTTTACGCCTTCAGAAGATCAATTAACTTGGACACTGAAGTTAAAAGA
TAATTTGAAATGGCATGATGGTAAGAAAATTACATCAGACGATATAGCATTTACATTCCAATCTATTTTAGATGAGAAGC
AAAATAGCTCAAGTCGTGAAAACTTTATTTTTAAAGGAAAGCCGCTTGAGGTGAAAAAGGTAGATGAGTTAACAACTCAA
TTCGTTTTACCACAAGTAAGCGCATCTTTCGAAGGTGTGATGAATGATTTCTTCCCAATTCCGAAACATGTATTTGAAGG
GGAAGCAGATTTAGCGAAGAGTAAGAAAAACTTACAACCTGTAGGGTCAGGTCCGTTTAAGTTTAAAGAATATAAATCAG
ATGAGTACGTTGCATTAGATCGATTCGATGATTATGTAGGTGGGAAAGCTAAATTAGACTCTATCGTATACCGAGTTGTA
AAAGACCGTAATACAGCAAATGTTTCACTGCAAAATGGTCAAATCAACATGAAGATGATTGAGCCGCAAGACTTTAAGAA
ACTAGATAGTACTGGGAACTTCTCAATGGTGACATTCCCTGAAGGTAGATTATTCTACTTATCTTATAACATGAATACAG
ATTTGATGAAGAAAAAAGAAGTGCGCCAAGCAATTGCGCATGCGTTAGATAAAAAAGAAATGATTAACTCAGCATTCGTT
TCAGGTGAATTTGCAGAACCAGCAAATTCAATCTTAACGCCAGACGCTATGTATTATGCGAAAGATATTAAAGACTATAA
GTATGATAAAAAAGAAGCGAAAGATTTATTAGCAAAAGCTGGCGTGAAAGATAACGAAAAAGTACGTGTGATGTATGTAA
CGAATAATAAAATCATGGAAAGCTTAGCGTTATATACACAACAAAAATTAAAAGAAGTTGGCTTAGAAGTTGAACTAAAT
GCATTAGATGCTAGTGCAGCAAGTGAAAAAGGCTTAGATAAAGAGAATAAAGAATATGACATTACATTTGGTGGTTACAT
AATGGGACCTGAGCCAGATTCATATAAGAGCTTATTCTTAAGTAATGCTGAATACAATTATGCACGATATAAAAACGCTG
ATTTCGATAAGTTATGGGAAGAAGCTGCAGTTGAAACAGATAAAACAAAACGCGCAGAGCTATACCATAAAATTCAAGAG
ACAGCTAGAGAAGACGTACCTTATCTACCAATCGCGTATCCGAAAGCAGTTATTGCAGTAGATAAAAAGTTTGATGGATT
AAAAGAAGCGAAAGCAATTCCTGTCACAATGTTTGAAGATCTATCTAAGATTTATGAAGTGAAATAA

Upstream 100 bases:

>100_bases
TGACAATACATACGGGTAGTGGTAATTTAAAACCTATAAGAAAACTCGGTATTAATCCCGAGTTTTCTTATAGGTGTAAA
TAGTCGAGAGGGGTCGAAAT

Downstream 100 bases:

>100_bases
GAAACAATAAAGAAGCTGGTGGAAATCAGCTTCTTTAGCTTGAGGGGGGAAGTTTGTGTATAAAGTAATTGCGAAGAGGC
TTTTAAATGCAATTCCGCTT

Product: oligopeptide ABC transporter, oligopeptide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MKKKLFVLPFVLLLFIALAACSGEKDSKQASTSKSGTPKDGGALTIGVSDNPDTMNPLYANDRVSLTVQQALYAPLYHME
DGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFIFKGKPLEVKKVDELTTQ
FVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAFV
SGEFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKAGVKDNEKVRVMYVTNNKIMESLALYTQQKLKEVGLEVELN
ALDASAASEKGLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
TAREDVPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK

Sequences:

>Translated_528_residues
MKKKLFVLPFVLLLFIALAACSGEKDSKQASTSKSGTPKDGGALTIGVSDNPDTMNPLYANDRVSLTVQQALYAPLYHME
DGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFIFKGKPLEVKKVDELTTQ
FVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAFV
SGEFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKAGVKDNEKVRVMYVTNNKIMESLALYTQQKLKEVGLEVELN
ALDASAASEKGLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
TAREDVPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK
>Mature_528_residues
MKKKLFVLPFVLLLFIALAACSGEKDSKQASTSKSGTPKDGGALTIGVSDNPDTMNPLYANDRVSLTVQQALYAPLYHME
DGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFIFKGKPLEVKKVDELTTQ
FVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAFV
SGEFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKAGVKDNEKVRVMYVTNNKIMESLALYTQQKLKEVGLEVELN
ALDASAASEKGLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
TAREDVPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=478, Percent_Identity=26.3598326359833, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1789966, Length=485, Percent_Identity=27.0103092783505, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1787762, Length=527, Percent_Identity=26.7552182163188, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789887, Length=510, Percent_Identity=24.5098039215686, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1787551, Length=500, Percent_Identity=22.2, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789397, Length=488, Percent_Identity=21.7213114754098, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787495, Length=441, Percent_Identity=23.5827664399093, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI87081878, Length=473, Percent_Identity=22.6215644820296, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59740; Mature: 59740

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLFVLPFVLLLFIALAACSGEKDSKQASTSKSGTPKDGGALTIGVSDNPDTMNPLYA
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
NDRVSLTVQQALYAPLYHMEDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSD
CCEEEEEEHHHHHCCHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCH
DIAFTFQSILDEKQNSSSRENFIFKGKPLEVKKVDELTTQFVLPQVSASFEGVMNDFFPI
HHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
CHHHHCCCHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEECHHHHCCCHHHHHHHHHHHH
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKE
HCCCCCEEEEECCEEEEEEECCHHHHHHCCCCCEEEEEECCCEEEEEEECCCHHHHHHHH
VRQAIAHALDKKEMINSAFVSGEFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHC
GVKDNEKVRVMYVTNNKIMESLALYTQQKLKEVGLEVELNALDASAASEKGLDKENKEYD
CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCCEEE
ITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
EEECCEEECCCCCCCHHHEECCCCCCHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
TAREDVPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK
HHHHCCCCCCCCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKLFVLPFVLLLFIALAACSGEKDSKQASTSKSGTPKDGGALTIGVSDNPDTMNPLYA
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
NDRVSLTVQQALYAPLYHMEDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSD
CCEEEEEEHHHHHCCHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCH
DIAFTFQSILDEKQNSSSRENFIFKGKPLEVKKVDELTTQFVLPQVSASFEGVMNDFFPI
HHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVV
CHHHHCCCHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEECHHHHCCCHHHHHHHHHHHH
KDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKE
HCCCCCEEEEECCEEEEEEECCHHHHHHCCCCCEEEEEECCCEEEEEEECCCHHHHHHHH
VRQAIAHALDKKEMINSAFVSGEFAEPANSILTPDAMYYAKDIKDYKYDKKEAKDLLAKA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHC
GVKDNEKVRVMYVTNNKIMESLALYTQQKLKEVGLEVELNALDASAASEKGLDKENKEYD
CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCCEEE
ITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQE
EEECCEEECCCCCCCHHHEECCCCCCHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
TAREDVPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDLSKIYEVK
HHHHCCCCCCCCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]