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The map label for this gene is nth [H]

Identifier: 218233114

GI number: 218233114

Start: 1528751

End: 1529398

Strand: Direct

Name: nth [H]

Synonym: BCB4264_A1603

Alternate gene names: 218233114

Gene position: 1528751-1529398 (Clockwise)

Preceding gene: 218232332

Following gene: 218233830

Centisome position: 28.21

GC content: 36.57

Gene sequence:

>648_bases
ATGTTAAATAAAACGCAAATTCGCTATTGTTTAGACACAATGGCGGATATGTATCCAGAAGCACATTGTGAATTAATTCA
TGAGAATCCTTTTGAATTAGTAATCGCGGTAGCACTATCTGCACAATGTACAGATGCACTTGTAAATAAAGTGACGAAAA
ATTTATTTCAAAAATATAAAACACCAGAAGATTATTTAAGTGTATCTTTGGAAGAGTTACAACAAGATATACGTTCTATT
GGGTTGTATAGAAATAAAGCAAAAAACATTCAAAAATTGTGTCAGATGTTGCTGGATGATTACAATGGAAAAGTTCCAGA
AGACCGTGACGAGTTGACGAAATTACCAGGAGTAGGGAGAAAAACAGCAAATGTAGTTGTTTCGGTAGCGTATGGCATCC
CGGCAATTGCTGTTGATACGCATGTAGAAAGAGTGAGTAAACGGTTAGCGATTTGTAGATGGAAAGATTCGGTGTTAGAA
GTAGAAAAAACATTAATGAAGAAAGTACCGATGGATGAGTGGGGAGTTACACATCATCGTATGATTTTCTTTGGACGTTA
TCACTGTAAAGCACAACGCCCACAATGTGAGGAGTGCAGACTGTTAGAAGTATGTCGTGAAGGAAAGAAGCGAATGAAGG
TGAAATAA

Upstream 100 bases:

>100_bases
GAATCAACAAAGAGCACAACAAATGACAAAACAAGAGACGAAATTTACTGGAAAAGTGCCTTTTTATAATTGGTTGGAGC
AGTAATATAGGAGGAAGTAT

Downstream 100 bases:

>100_bases
AGGATGGAGCGAGTTATAGAAATACCGGAAGAATTTCACTGTTTACCATTTTTTAAAGAAAGTGTAAATTTAATTAAGTA
TCATACAGACAATTCTTTTG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MLNKTQIRYCLDTMADMYPEAHCELIHENPFELVIAVALSAQCTDALVNKVTKNLFQKYKTPEDYLSVSLEELQQDIRSI
GLYRNKAKNIQKLCQMLLDDYNGKVPEDRDELTKLPGVGRKTANVVVSVAYGIPAIAVDTHVERVSKRLAICRWKDSVLE
VEKTLMKKVPMDEWGVTHHRMIFFGRYHCKAQRPQCEECRLLEVCREGKKRMKVK

Sequences:

>Translated_215_residues
MLNKTQIRYCLDTMADMYPEAHCELIHENPFELVIAVALSAQCTDALVNKVTKNLFQKYKTPEDYLSVSLEELQQDIRSI
GLYRNKAKNIQKLCQMLLDDYNGKVPEDRDELTKLPGVGRKTANVVVSVAYGIPAIAVDTHVERVSKRLAICRWKDSVLE
VEKTLMKKVPMDEWGVTHHRMIFFGRYHCKAQRPQCEECRLLEVCREGKKRMKVK
>Mature_215_residues
MLNKTQIRYCLDTMADMYPEAHCELIHENPFELVIAVALSAQCTDALVNKVTKNLFQKYKTPEDYLSVSLEELQQDIRSI
GLYRNKAKNIQKLCQMLLDDYNGKVPEDRDELTKLPGVGRKTANVVVSVAYGIPAIAVDTHVERVSKRLAICRWKDSVLE
VEKTLMKKVPMDEWGVTHHRMIFFGRYHCKAQRPQCEECRLLEVCREGKKRMKVK

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=214, Percent_Identity=28.5046728971963, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI190358497, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI6912520, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI115298648, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI115298654, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI115298652, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI115298650, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=44.9760765550239, Blast_Score=174, Evalue=3e-45,
Organism=Escherichia coli, GI1789331, Length=187, Percent_Identity=24.5989304812834, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=172, Percent_Identity=36.046511627907, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319304, Length=177, Percent_Identity=27.683615819209, Blast_Score=69, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550361, Length=190, Percent_Identity=31.0526315789474, Blast_Score=98, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24874; Mature: 24874

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKTQIRYCLDTMADMYPEAHCELIHENPFELVIAVALSAQCTDALVNKVTKNLFQKYK
CCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPEDYLSVSLEELQQDIRSIGLYRNKAKNIQKLCQMLLDDYNGKVPEDRDELTKLPGVGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCC
KTANVVVSVAYGIPAIAVDTHVERVSKRLAICRWKDSVLEVEKTLMKKVPMDEWGVTHHR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHH
MIFFGRYHCKAQRPQCEECRLLEVCREGKKRMKVK
HHEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNKTQIRYCLDTMADMYPEAHCELIHENPFELVIAVALSAQCTDALVNKVTKNLFQKYK
CCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPEDYLSVSLEELQQDIRSIGLYRNKAKNIQKLCQMLLDDYNGKVPEDRDELTKLPGVGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCC
KTANVVVSVAYGIPAIAVDTHVERVSKRLAICRWKDSVLEVEKTLMKKVPMDEWGVTHHR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHH
MIFFGRYHCKAQRPQCEECRLLEVCREGKKRMKVK
HHEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]