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The map label for this gene is malK [H]

Identifier: 218232726

GI number: 218232726

Start: 594141

End: 595745

Strand: Direct

Name: malK [H]

Synonym: BCB4264_A0614

Alternate gene names: 218232726

Gene position: 594141-595745 (Clockwise)

Preceding gene: 218232154

Following gene: 218231939

Centisome position: 10.96

GC content: 34.58

Gene sequence:

>1605_bases
ATGAAAAAAAGAAGAAGACTATGGAATTTATGGAAAACGATTACATTGTTAGTTTGTACAGTTGTGATTTTTTCTTTACT
TGTGACAGATATATTAATTAGTCACAATGTAGAACGGACAACGGAGGATAACCAAGCAGAGAAAGCAAAAACAATTGCAC
ACATTGTGGCGAATGATTCGATTGTAATCGATGGCTTGATTGGGAAAGCAGATACTTCTGCAATCCAAACGTATACAAAT
AGGATATTAAAAAACACAGGTGTTCAATTTATTGTAGTTATGGACATGAATGGAATAAGAAAATCACATCCAAATCCTCA
AAAAATAGGTCATCATTTTATTGGGGGAGATGAAGGGCCTGCATTGAAAGGAAAGGAACATGTATCACTGGCAGAAGGAA
CTTTAGGTATTTCAATGCGAGTGTTTGTACCTGTATTTTCTGAAACGGGTGAACAGCTTGGAGCGGTGGCGGTCGGTATT
TCAGCAGATAATGTAAAAGAGAGAGTTAAGGAAAGTAGATATATCATTTATATTGGAGTTGGAGTTGGAGTATTAGTCGG
AATTATAGGAGCGATATTGCTAGCTAGACATATAAAGAAAAGCTTATTCGGTCTTGAGCCGCACAGGATAGCAAAAATTC
TTGAAGAAAGAAATACGATGCTACAATCTGTAAAGGAAGGTATTATTGCTGTAGATAAAGAGGCTAGAGTAACGCTAATT
AATAATGAAGCGAAACGTCTATTTAAAAAAAGCGGACTTGAAGAAGATTTTATCGGTAAAGATGTTGAGTTGTATATGCC
GAATTCACGTATAAAAGAAGTATTGCAAACGGGAGAAGTACAATTAAACGAAGAACAAAATATTTATGGAATTACGATTG
TGACGAATCGAGTTCCTTTATATGTAAAAGGAGAAATAGTTGGCGCAATTGCAACATTTCGTGATAAAACAGAGATTCGA
AAACTAGCAGAGGAACTAACTGGTATTAGACTATATGCAGAAGCATTACGGGCACAATCTCATGAGTTTATGAATAAGAT
GCATGTCGTATTAGGGCTTACACATATGAAACAGTATGAAGAATTACAGAAGTATATAAGCGGTATGGTATCAGAGCATC
AATATGAAATTGGTGGAGTTATGAAAAGAATAAAAAATCCAGTATTTGCTGGTTTTTTACTAGGTAAGCTCAGCTATGCT
AGAGAGAAGAATATCAAATTAATTATAAGTGAAGATTCTCATATGCCGGAAATAGATGATGAAAGTATTACTCATGAACT
TATTACGATTGTGGGAAATTTAATTGATAATGCGTTAGAGGCAGTGACGAATTGTGAAAAGAAGCAAGTTGAAGTTGAGA
TACAATATGGGGATATATTAACTATTACAGTACAAGATACGGGAAAAGGTATACAAGAAAAAGAAATAGAGGCATTATTT
ACGAAAGGTTATTCAACAAAGGGAGATAACCGCGGTTATGGTTTGTATCTTGTAAAAGAAAGTATACAGCGAATAAATGG
GGAAATTGATATACATTCATTAGTAGGAAAGGGAACAACGATAACAATTGAAATACCTAAAGGTAGGGATGAGAGGCAAA
TATGA

Upstream 100 bases:

>100_bases
GACTAGTAAATTTAAAGTATAATAACAGCAGATAAGTAAGGTGAAAATAATATCACCTTACTTATTTTTCTATTCAGCAA
TTAGGGGAGTCATTAACGGG

Downstream 100 bases:

>100_bases
TTAAAGTTTTAATTGTAGAAGATGACCCGATGGTAGCAATGTTAAATACGCATTATTTAGAGCAAGTAGGAGGGTTTGAA
CTTGTTCAAGCAGTTAACTC

Product: sensory histidine kinase DcuS

Products: NA

Alternate protein names: Malate kinase sensor [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKKRRRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDNQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTN
RILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGI
SADNVKERVKESRYIIYIGVGVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLI
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKGEIVGAIATFRDKTEIR
KLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKNPVFAGFLLGKLSYA
REKNIKLIISEDSHMPEIDDESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGDILTITVQDTGKGIQEKEIEALF
TKGYSTKGDNRGYGLYLVKESIQRINGEIDIHSLVGKGTTITIEIPKGRDERQI

Sequences:

>Translated_534_residues
MKKRRRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDNQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTN
RILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGI
SADNVKERVKESRYIIYIGVGVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLI
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKGEIVGAIATFRDKTEIR
KLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKNPVFAGFLLGKLSYA
REKNIKLIISEDSHMPEIDDESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGDILTITVQDTGKGIQEKEIEALF
TKGYSTKGDNRGYGLYLVKESIQRINGEIDIHSLVGKGTTITIEIPKGRDERQI
>Mature_534_residues
MKKRRRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDNQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTN
RILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGI
SADNVKERVKESRYIIYIGVGVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLI
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKGEIVGAIATFRDKTEIR
KLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKNPVFAGFLLGKLSYA
REKNIKLIISEDSHMPEIDDESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGDILTITVQDTGKGIQEKEIEALF
TKGYSTKGDNRGYGLYLVKESIQRINGEIDIHSLVGKGTTITIEIPKGRDERQI

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=526, Percent_Identity=40.1140684410646, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1786837, Length=524, Percent_Identity=30.5343511450382, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1790436, Length=129, Percent_Identity=31.7829457364341, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59674; Mature: 59674

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRRRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDNQAEKAKTIAHIVANDS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
IVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGP
EEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCC
ALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGISADNVKERVKESRYIIYIGV
CCCCCCCCEEECCCCCEEEEEEEEEHHHCCHHHCEEEEECCHHHHHHHHHHCCEEEEEEC
GVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEE
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPL
CHHHHHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHCCCEEECCCCCEEEEEEEECCCCE
YVKGEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYE
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELQKYISGMVSEHQYEIGGVMKRIKNPVFAGFLLGKLSYAREKNIKLIISEDSHMPEIDD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
ESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGDILTITVQDTGKGIQEKEIEALF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCCCCHHHHHHHHH
TKGYSTKGDNRGYGLYLVKESIQRINGEIDIHSLVGKGTTITIEIPKGRDERQI
HCCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MKKRRRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDNQAEKAKTIAHIVANDS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
IVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGP
EEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCC
ALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGISADNVKERVKESRYIIYIGV
CCCCCCCCEEECCCCCEEEEEEEEEHHHCCHHHCEEEEECCHHHHHHHHHHCCEEEEEEC
GVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEE
NNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPL
CHHHHHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHCCCEEECCCCCEEEEEEEECCCCE
YVKGEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYE
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELQKYISGMVSEHQYEIGGVMKRIKNPVFAGFLLGKLSYAREKNIKLIISEDSHMPEIDD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
ESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGDILTITVQDTGKGIQEKEIEALF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCCCCHHHHHHHHH
TKGYSTKGDNRGYGLYLVKESIQRINGEIDIHSLVGKGTTITIEIPKGRDERQI
HCCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]