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The map label for this gene is rumA [C]

Identifier: 218231359

GI number: 218231359

Start: 477110

End: 478489

Strand: Direct

Name: rumA [C]

Synonym: BCB4264_A0491

Alternate gene names: 218231359

Gene position: 477110-478489 (Clockwise)

Preceding gene: 218232083

Following gene: 218230891

Centisome position: 8.8

GC content: 37.9

Gene sequence:

>1380_bases
ATGATACAAAAGCAACATGAGAGTAAGTTGGAAGTTGGTCAAACGTTTCCTGTGACAATTAAACGTCTTGGGATTAACGG
AGAAGGCGTTGGTTATTTTAAGAGACAAGTTGTTTTCATTCCAGGGGCATTACCAGGAGAAGAAGTTGTTGCAGAAACAA
CGAAAATTCAGCGTGGCTTCGCTGAAGCGAAAGTGAAAAAAGTTCGTAAAGCTTCACCGCATCGTGTGAAAGCACCGTGT
CCAGTATATGAGGAGTGTGGCGGTTGTCAGCTGCAACATTTAGATTATAAAGAACAATTGAATCAAAAGCGTGATATCGT
TGTACAAGCATTTGAGAAGTACATGAAAAATAGTATGGAAGAGAAAATTCGCCCAACGCTTGGCATGGAAAATCCATGGC
ATTATCGTAATAAGAGTCAATTACAAGTGGGGCGTAAAGACGAAAAGGTTATTACAGGGCTGTATAAACAAAACTCACAT
CAGTTAATTGATATCGCTCACTGTATGATTCAACATAAAGCAACGAATGAAGCGACAAAAGTTGTAAGACGTATTTTAGA
AAAATTAAATGTTTCTATTTACAATGAGAAAAAACAAAAAGGTTTAGTACGCACAATTGTAACACGTACTGCAGTTCAAA
CAGGGGAAGTACAAGTTACACTTATTACAACAAAAGAAGAATTACCAAATAAAGAACAATTTATTGCAGAAGTACAAAAA
CAGATGCCAGCGGTTAAATCAATTATGCAAAATGTAAACTGGCGTAAAACATCTGTTATTTTTGGCGACAAAACATTTAA
ATTAGCTGGAAAAGAAGTCATTCAAGAAACACTTGGCGATTTATCATTTGAATTATCAGCACGTGCATTCTTCCAGTTGA
ATCCAGAGCAAACAGTTGTTTTGTATAATGAAGCGAAAAAAGCAGCTGCGTTAACAGGAAACGAGAAGATTGTGGATGCG
TACTGTGGTGTTGGTACAATCGGTCTTTGGCTTGCAAATGATGCAGCGGAAGTACGTGGTATGGATGTAATTCCAGAAGC
AATTGCAGATGCAAGAAAAAATGCAAAGCGTCACGGATTTACAAATACGAAATATGAAGCAGGTAAAGCTGAACAATGGT
TACCGAAATGGGTAAAAGAAGGATGGCGTCCAGATGTAATTGTTGTCGACCCACCACGTACAGGTTGCGATGATAAATTA
CTGGAAACAATTTTAAAAGTGAAGCCGAAACAAGTTGTGTACGTGTCTTGTAATCCTTCTTCATTAGCACGTGATGTACA
AGCATTAATGAAGAGCTATGAAGTGGAGTATGTGCAACCAGTTGATATGTTCCCGCATACAGCTCACGTAGAAAATGTAG
TGAAGCTTGTTAGAAAGTAA

Upstream 100 bases:

>100_bases
CCTGATGATGCATTTTTAGAAAAAGTGAAACAAGAGTGTGAACCATACTGCAGTTATGCAGCGTTATATTTATGGAAAAG
TATAGAGTAGAGGTGTAATA

Downstream 100 bases:

>100_bases
AGTTTATTAATATTCCCTCATGGTATGAGGGAATATTTTCTATGAAGGAGAATATTATGCATAATTATAAATTAACGATT
CAGTACGACGGCGCACGTTT

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MIQKQHESKLEVGQTFPVTIKRLGINGEGVGYFKRQVVFIPGALPGEEVVAETTKIQRGFAEAKVKKVRKASPHRVKAPC
PVYEECGGCQLQHLDYKEQLNQKRDIVVQAFEKYMKNSMEEKIRPTLGMENPWHYRNKSQLQVGRKDEKVITGLYKQNSH
QLIDIAHCMIQHKATNEATKVVRRILEKLNVSIYNEKKQKGLVRTIVTRTAVQTGEVQVTLITTKEELPNKEQFIAEVQK
QMPAVKSIMQNVNWRKTSVIFGDKTFKLAGKEVIQETLGDLSFELSARAFFQLNPEQTVVLYNEAKKAAALTGNEKIVDA
YCGVGTIGLWLANDAAEVRGMDVIPEAIADARKNAKRHGFTNTKYEAGKAEQWLPKWVKEGWRPDVIVVDPPRTGCDDKL
LETILKVKPKQVVYVSCNPSSLARDVQALMKSYEVEYVQPVDMFPHTAHVENVVKLVRK

Sequences:

>Translated_459_residues
MIQKQHESKLEVGQTFPVTIKRLGINGEGVGYFKRQVVFIPGALPGEEVVAETTKIQRGFAEAKVKKVRKASPHRVKAPC
PVYEECGGCQLQHLDYKEQLNQKRDIVVQAFEKYMKNSMEEKIRPTLGMENPWHYRNKSQLQVGRKDEKVITGLYKQNSH
QLIDIAHCMIQHKATNEATKVVRRILEKLNVSIYNEKKQKGLVRTIVTRTAVQTGEVQVTLITTKEELPNKEQFIAEVQK
QMPAVKSIMQNVNWRKTSVIFGDKTFKLAGKEVIQETLGDLSFELSARAFFQLNPEQTVVLYNEAKKAAALTGNEKIVDA
YCGVGTIGLWLANDAAEVRGMDVIPEAIADARKNAKRHGFTNTKYEAGKAEQWLPKWVKEGWRPDVIVVDPPRTGCDDKL
LETILKVKPKQVVYVSCNPSSLARDVQALMKSYEVEYVQPVDMFPHTAHVENVVKLVRK
>Mature_459_residues
MIQKQHESKLEVGQTFPVTIKRLGINGEGVGYFKRQVVFIPGALPGEEVVAETTKIQRGFAEAKVKKVRKASPHRVKAPC
PVYEECGGCQLQHLDYKEQLNQKRDIVVQAFEKYMKNSMEEKIRPTLGMENPWHYRNKSQLQVGRKDEKVITGLYKQNSH
QLIDIAHCMIQHKATNEATKVVRRILEKLNVSIYNEKKQKGLVRTIVTRTAVQTGEVQVTLITTKEELPNKEQFIAEVQK
QMPAVKSIMQNVNWRKTSVIFGDKTFKLAGKEVIQETLGDLSFELSARAFFQLNPEQTVVLYNEAKKAAALTGNEKIVDA
YCGVGTIGLWLANDAAEVRGMDVIPEAIADARKNAKRHGFTNTKYEAGKAEQWLPKWVKEGWRPDVIVVDPPRTGCDDKL
LETILKVKPKQVVYVSCNPSSLARDVQALMKSYEVEYVQPVDMFPHTAHVENVVKLVRK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=350, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI51173878, Length=350, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI269784678, Length=385, Percent_Identity=25.974025974026, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI269784680, Length=208, Percent_Identity=31.7307692307692, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI269784676, Length=208, Percent_Identity=31.7307692307692, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI20149688, Length=208, Percent_Identity=31.7307692307692, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1789148, Length=458, Percent_Identity=26.8558951965066, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1787083, Length=383, Percent_Identity=24.8041775456919, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1790403, Length=211, Percent_Identity=25.5924170616114, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71997438, Length=165, Percent_Identity=32.7272727272727, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322909, Length=429, Percent_Identity=24.9417249417249, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24666579, Length=445, Percent_Identity=28.5393258426966, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 52008; Mature: 52008

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKQHESKLEVGQTFPVTIKRLGINGEGVGYFKRQVVFIPGALPGEEVVAETTKIQRGF
CCCCCCCCHHHCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHH
AEAKVKKVRKASPHRVKAPCPVYEECGGCQLQHLDYKEQLNQKRDIVVQAFEKYMKNSME
HHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEECCCHHHHHCHHHHHHHHHHHHHHHHHHH
EKIRPTLGMENPWHYRNKSQLQVGRKDEKVITGLYKQNSHQLIDIAHCMIQHKATNEATK
HHHCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHH
VVRRILEKLNVSIYNEKKQKGLVRTIVTRTAVQTGEVQVTLITTKEELPNKEQFIAEVQK
HHHHHHHHCCCEEECCHHHHHHHHHHHHHHHEECCCEEEEEEECHHHCCCHHHHHHHHHH
QMPAVKSIMQNVNWRKTSVIFGDKTFKLAGKEVIQETLGDLSFELSARAFFQLNPEQTVV
HHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCEEE
LYNEAKKAAALTGNEKIVDAYCGVGTIGLWLANDAAEVRGMDVIPEAIADARKNAKRHGF
EEECCCHHHHCCCCCHHHHHHCCCCHHHEEEECCHHHHCCCHHHHHHHHHHHHHHHHCCC
TNTKYEAGKAEQWLPKWVKEGWRPDVIVVDPPRTGCDDKLLETILKVKPKQVVYVSCNPS
CCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCHH
SLARDVQALMKSYEVEYVQPVDMFPHTAHVENVVKLVRK
HHHHHHHHHHHHCCCCEECCHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIQKQHESKLEVGQTFPVTIKRLGINGEGVGYFKRQVVFIPGALPGEEVVAETTKIQRGF
CCCCCCCCHHHCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHH
AEAKVKKVRKASPHRVKAPCPVYEECGGCQLQHLDYKEQLNQKRDIVVQAFEKYMKNSME
HHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEECCCHHHHHCHHHHHHHHHHHHHHHHHHH
EKIRPTLGMENPWHYRNKSQLQVGRKDEKVITGLYKQNSHQLIDIAHCMIQHKATNEATK
HHHCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHH
VVRRILEKLNVSIYNEKKQKGLVRTIVTRTAVQTGEVQVTLITTKEELPNKEQFIAEVQK
HHHHHHHHCCCEEECCHHHHHHHHHHHHHHHEECCCEEEEEEECHHHCCCHHHHHHHHHH
QMPAVKSIMQNVNWRKTSVIFGDKTFKLAGKEVIQETLGDLSFELSARAFFQLNPEQTVV
HHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCEEE
LYNEAKKAAALTGNEKIVDAYCGVGTIGLWLANDAAEVRGMDVIPEAIADARKNAKRHGF
EEECCCHHHHCCCCCHHHHHHCCCCHHHEEEECCHHHHCCCHHHHHHHHHHHHHHHHCCC
TNTKYEAGKAEQWLPKWVKEGWRPDVIVVDPPRTGCDDKLLETILKVKPKQVVYVSCNPS
CCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCHH
SLARDVQALMKSYEVEYVQPVDMFPHTAHVENVVKLVRK
HHHHHHHHHHHHCCCCEECCHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]