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The map label for this gene is truA2 [H]

Identifier: 218230891

GI number: 218230891

Start: 478546

End: 479283

Strand: Direct

Name: truA2 [H]

Synonym: BCB4264_A0492

Alternate gene names: 218230891

Gene position: 478546-479283 (Clockwise)

Preceding gene: 218231359

Following gene: 218233836

Centisome position: 8.83

GC content: 33.88

Gene sequence:

>738_bases
ATGCATAATTATAAATTAACGATTCAGTACGACGGCGCACGTTTTAAAGGATGGCAACGTCTCGGTAATAACGATAATAC
AATTCAAGGAAAAATCGAAAGTGTCATATCTGAAATGGTCGGAAAAGAAATTGAAATTATCGGTTGTAGTAGAACAGACG
CTGGTGTACATGCTTTGAATCAAGTTGCTAACTTTCAAAGTGATGAGAAGTTAGTGGAACATAAAGTGAAAAAATATTTA
AATCAATATTTACCTAACGATATTAGCATTACGAATGTGGAAGAAGTACACGATCGTTTCCATGCTCGTTACAATTCTAA
AGCAAAAACATATCTGTATAAAATTTGGAATGAAGAGCATACAAATCCATTTATGCGTAAATACAGCATGCATGTGAATA
AAAAATTAAATGTGAAAAGCATGAAAGCAGCTGCAAAACATTTAGTTGGTTCACATGACTTTACTGCTTTTTCAAATGCG
AAATCAAAGAAAAAATCTATGGTTCGAGAAGTATATACACTTGATGTGATGGAAGAGGCAGGGTTTGTACAAATTAGAGT
AAGTGGTAACGGCTTCCTTCATAATATGGTAAGAAAAATTGTCGGGGCATTAATTGAAGTTGGATTAGGACAATTAGATG
CTGAAGCAATTCCAAACATTTTAGAGGAAAAGCAGCGCAATCAAATTAATTGTCTTGCTGAGGCAAGTGGGTTGTATTTA
GAGAATGTTGAGTTTTAA

Upstream 100 bases:

>100_bases
ATACAGCTCACGTAGAAAATGTAGTGAAGCTTGTTAGAAAGTAAAGTTTATTAATATTCCCTCATGGTATGAGGGAATAT
TTTCTATGAAGGAGAATATT

Downstream 100 bases:

>100_bases
TGGCAAATAAAAAAATCGGGCTAAGTAGCTCGATTTTTTTAGTATTACGAAGATTTTTCGCTTTTTATACGTAGCTTTAA
ATTCCAAAAAGCAGCAAGCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 2; tRNA-uridine isomerase I 2 [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MHNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKEIEIIGCSRTDAGVHALNQVANFQSDEKLVEHKVKKYL
NQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEHTNPFMRKYSMHVNKKLNVKSMKAAAKHLVGSHDFTAFSNA
KSKKKSMVREVYTLDVMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPNILEEKQRNQINCLAEASGLYL
ENVEF

Sequences:

>Translated_245_residues
MHNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKEIEIIGCSRTDAGVHALNQVANFQSDEKLVEHKVKKYL
NQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEHTNPFMRKYSMHVNKKLNVKSMKAAAKHLVGSHDFTAFSNA
KSKKKSMVREVYTLDVMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPNILEEKQRNQINCLAEASGLYL
ENVEF
>Mature_245_residues
MHNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKEIEIIGCSRTDAGVHALNQVANFQSDEKLVEHKVKKYL
NQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEHTNPFMRKYSMHVNKKLNVKSMKAAAKHLVGSHDFTAFSNA
KSKKKSMVREVYTLDVMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPNILEEKQRNQINCLAEASGLYL
ENVEF

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=280, Percent_Identity=28.2142857142857, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1788657, Length=240, Percent_Identity=34.1666666666667, Blast_Score=153, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI71985046, Length=266, Percent_Identity=28.1954887218045, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27993; Mature: 27993

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKEIEIIGCSRTDAGVHALN
CCCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
QVANFQSDEKLVEHKVKKYLNQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEH
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHEECCCCC
TNPFMRKYSMHVNKKLNVKSMKAAAKHLVGSHDFTAFSNAKSKKKSMVREVYTLDVMEEA
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPNILEEKQRNQINCLAEASGLYL
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEE
ENVEF
ECCCC
>Mature Secondary Structure
MHNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKEIEIIGCSRTDAGVHALN
CCCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
QVANFQSDEKLVEHKVKKYLNQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEH
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHEECCCCC
TNPFMRKYSMHVNKKLNVKSMKAAAKHLVGSHDFTAFSNAKSKKKSMVREVYTLDVMEEA
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPNILEEKQRNQINCLAEASGLYL
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEE
ENVEF
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]