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The map label for this gene is leuA [H]

Identifier: 218231353

GI number: 218231353

Start: 1394732

End: 1396234

Strand: Direct

Name: leuA [H]

Synonym: BCB4264_A1453

Alternate gene names: 218231353

Gene position: 1394732-1396234 (Clockwise)

Preceding gene: 218233670

Following gene: 218231765

Centisome position: 25.74

GC content: 39.12

Gene sequence:

>1503_bases
ATGGATACAACGCTTCGTGATGGCGAACAATCACCAGGAGTAAATTTAAATGAACAAGAGAAATTACAAATTGCTAGGCA
ATTAGAGAGGCTTGGTATTCATGTAATGGAAGCTGGATTCGCGGCAGCTTCCGAAGGCGACTTCCAATCGGTAAAACGTA
TTGCGAATACAATTCAAAATGCTACGGTTATGAGTTTAGCTAGAGCGAAAGAAAGTGACATTCGAAGAGCATATGAAGCT
GTGAAAGGCGCTGTATCTCCTCGTCTACACGTATTTCTAGCTACGAGTGATATTCATATGAAATATAAACTTTGTATGTC
AAAAGAAGATGTGTTAAATAGTATTCATCGCTCGGTTACGCTCGGAAAATCATTATTTCCGACAGTACAATTTTCTGCAG
AAGATGCGACAAGAACATCGAGAGCATTTTTAGCTGAAGCAGTAGAAATTGCGATTCGAGCAGGAGCGAATGTAATTAAT
ATTCCGGATACAGTTGGATATACAAATCCGGAAGAGTATTATTCTCTTTTTAAATACTTACAAGAATCCGTTCCATCATA
TGAAAAAGCGATTTTTTCTTGTCATTGTCACGATGATCTTGGTATGGCAGTAGCAAATTCGTTAGCTGCGGTTGAAGGGG
GAGCATTGCAAGTAGAAGGAACGATTAATGGCATTGGAGAAAGAGCAGGGAATGCAGCGTTAGAAGAAGTTGCGGTAGCT
CTTCATATTAGAAAAGATTTTTATAAAGCAGAGCCTTCGATGACTTTAAAAGAAATTAAGGCGACGAGTATATTAGTAAG
CAGGTTAACGGGTATGGTTGTACCGAAAAATAAAGCAATCGTTGGAGCGAATGCATTTGCTCATGAATCGGGCATCCACC
AAGACGGGGTTTTAAAAGAAGTAACAACTTATGAAATTATCGAACCAGCGCTTATAGGAGAATCTCAAAATCTCTTTGTA
CTTGGGAAACACTCTGGCCGTCATGCGTTTACGGAAAAAATGAAAGAGCTTGGTTATGAATTTACAAATGAAGAGCGAGA
TGCGGCATTCGAAGCATTTAAAAAATTGGCTGATCGTAAAAAGGAAATTACTGAAGAAGATTTACGCGCACTTATGCTTG
GTGAAGCAGCATTTGCAGCTCAGCAATATAACATTACGCAATTACAAGTGCATTTCGTATCAAATAGTACACAATGCGCA
ACAGTTGTGTTGAAGGATGAGGAAGGAAATGTATATGAAGATGCAGCCACTGGTTCTGGTAGTATTGAAGCGATATACAA
TGCGATTCAAAGAATTTTAGGATTAGAATGTGAATTGGCAGATTATCGCATACAATCTATTACACAAGGTCAAGATGCAC
TTGCTCACGTTCATGTTGAATTAAAAGAAGGTAGTCACCAAATATCAGGTTTCGGAGTTGCGCAAGACGTATTGGAAGCG
TCGGCGAGAGCTTATGTCCATGCAGCAGGGAAATTAAAATCTCTTATAGCGCTTGTGAAATAA

Upstream 100 bases:

>100_bases
CATGAAATCGAAGTAGTTGGACGTAAATTACGTGAAATGATGCCTTTCGTACAGCCGCGAGTAAAGGTAGGGGTTAAATA
ATATGAAGCAGGTTTTGTTC

Downstream 100 bases:

>100_bases
ATAATTGATAGGTGAGAGAAGCTTTCCTCACCTATCAAATTAAAGTTCTGAAAATTCGGTTAAAAGGAGTGTTAAAAATG
GAAAAACGTATCGTTTGTTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQNATVMSLARAKESDIRRAYEA
VKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLNSIHRSVTLGKSLFPTVQFSAEDATRTSRAFLAEAVEIAIRAGANVIN
IPDTVGYTNPEEYYSLFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAALEEVAVA
LHIRKDFYKAEPSMTLKEIKATSILVSRLTGMVVPKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIEPALIGESQNLFV
LGKHSGRHAFTEKMKELGYEFTNEERDAAFEAFKKLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCA
TVVLKDEEGNVYEDAATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGSHQISGFGVAQDVLEA
SARAYVHAAGKLKSLIALVK

Sequences:

>Translated_500_residues
MDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQNATVMSLARAKESDIRRAYEA
VKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLNSIHRSVTLGKSLFPTVQFSAEDATRTSRAFLAEAVEIAIRAGANVIN
IPDTVGYTNPEEYYSLFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAALEEVAVA
LHIRKDFYKAEPSMTLKEIKATSILVSRLTGMVVPKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIEPALIGESQNLFV
LGKHSGRHAFTEKMKELGYEFTNEERDAAFEAFKKLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCA
TVVLKDEEGNVYEDAATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGSHQISGFGVAQDVLEA
SARAYVHAAGKLKSLIALVK
>Mature_500_residues
MDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQNATVMSLARAKESDIRRAYEA
VKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLNSIHRSVTLGKSLFPTVQFSAEDATRTSRAFLAEAVEIAIRAGANVIN
IPDTVGYTNPEEYYSLFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAALEEVAVA
LHIRKDFYKAEPSMTLKEIKATSILVSRLTGMVVPKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIEPALIGESQNLFV
LGKHSGRHAFTEKMKELGYEFTNEERDAAFEAFKKLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCA
TVVLKDEEGNVYEDAATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGSHQISGFGVAQDVLEA
SARAYVHAAGKLKSLIALVK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=488, Percent_Identity=46.1065573770492, Blast_Score=401, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6320071, Length=359, Percent_Identity=28.9693593314763, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320019, Length=360, Percent_Identity=28.8888888888889, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6324225, Length=529, Percent_Identity=24.5746691871456, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324682, Length=530, Percent_Identity=24.7169811320755, Blast_Score=128, Evalue=2e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 54566; Mature: 54566

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHHHHHHHH
ATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLNSIHRSVT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEHHHHCCHHHHHHHHHHHHH
LGKSLFPTVQFSAEDATRTSRAFLAEAVEIAIRAGANVINIPDTVGYTNPEEYYSLFKYL
HCCHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHH
QESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAALEEVAVA
HHHCCHHHHHHEEEECCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCHHHHHHHHHH
LHIRKDFYKAEPSMTLKEIKATSILVSRLTGMVVPKNKAIVGANAFAHESGIHQDGVLKE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCEEECCCCEEEECCHHHHCCCCCCCCCHHH
VTTYEIIEPALIGESQNLFVLGKHSGRHAFTEKMKELGYEFTNEERDAAFEAFKKLADRK
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVYEDAATGSG
HHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCEEECCCCCCC
SIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGSHQISGFGVAQDVLEA
CHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHH
SARAYVHAAGKLKSLIALVK
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHHHHHHHH
ATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLNSIHRSVT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEHHHHCCHHHHHHHHHHHHH
LGKSLFPTVQFSAEDATRTSRAFLAEAVEIAIRAGANVINIPDTVGYTNPEEYYSLFKYL
HCCHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHH
QESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAALEEVAVA
HHHCCHHHHHHEEEECCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCHHHHHHHHHH
LHIRKDFYKAEPSMTLKEIKATSILVSRLTGMVVPKNKAIVGANAFAHESGIHQDGVLKE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCEEECCCCEEEECCHHHHCCCCCCCCCHHH
VTTYEIIEPALIGESQNLFVLGKHSGRHAFTEKMKELGYEFTNEERDAAFEAFKKLADRK
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVYEDAATGSG
HHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCEEECCCCCCC
SIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGSHQISGFGVAQDVLEA
CHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHH
SARAYVHAAGKLKSLIALVK
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA