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The map label for this gene is gatB [H]

Identifier: 21675024

GI number: 21675024

Start: 2083861

End: 2085288

Strand: Reverse

Name: gatB [H]

Synonym: CT2215

Alternate gene names: 21675024

Gene position: 2085288-2083861 (Counterclockwise)

Preceding gene: 21675025

Following gene: 21675022

Centisome position: 96.77

GC content: 60.08

Gene sequence:

>1428_bases
ATGAACTATGAAATTGTCGTGGGCCTCGAGGTCCACTGTCAGCTCAATACCGAAAGCAAGGCATTCTGCGGCTGTTCTGC
CAAGTTCGGCAAACCGGCCAACACCAACGTCTGTCCGGTCTGCCTGGCGCTTCCCGGCGCGCTGCCGGTGCTGAACGCCC
GCGTGGTTGAGGACGCCGTCAAGCTCGGCCTGGCCACCAACTGCACCATCGCCCGTCACTCGATTCTGGCGCGCAAGAAC
TACTTCTATCCTGACCTTCCCAAGGGCTACCAGATTTCGCAGTACGAAGAGCCGATCTGCTCGGAGGGAGTGATCCATAT
CGATCTCGAAGAGGGGGGCAAGGATGTTCGTCTGGTGAGGATTCACATTGAGGAGGACGCGGGTAAATCGATCCACGACA
TCGGCGACGACACCTACATCGACGTGAACCGTTGCGGCGTGCCGCTGCTGGAGATCGTGAGCTACCCCGACATGCGCACG
CCGAAGGAGGCCTCGGCCTACCTGCAGAAGCTGCGCCAGATCGTCAGGTACCTCGGTATCTCCGACGGCAACATGGAGGA
GGGTAGCCTTCGCTGCGACGCCAACGTCTCGGTCAGGCCGGTTGGTGCGACCGAGTACGGCACCCGCACGGAGATCAAGA
ACATGAACTCGTTCCGCAACGTCGAGCGCGCCATCGAGTACGAAGCCAAGCGCCACATCGAGGTGATCGAGGGCGGCGGC
ACGATTGTGCAGGAGACGCGCCTGTGGGATGCCGACAAGCTCGAAACCCGTTCGATGCGCGGCAAGGAGCACGCCCACGA
CTACCGCTACTTTCCTGATCCGGACCTTGTGCCGGTGCTGGTAGACGACGGTATGATCCGCCGGATGCAGGAGGAGTTGC
CGGAGTTCCCGGAAGATCGCGCGGCGCGTTTCGTTTCCGAGTTCGGCATTCCGGCGTACGACGCTGGCGTTATCACCGTG
GACCGAGAGCTGGCCGACTATTTCGAATCAACGGTCAAGGTGTCGGGCGACGCCAAAGCCTCGTCGAACTGGGTGATGGG
TGAAGTGATGCGCACGCTGAAGGAGAAGTATCTCGACATTCACAAATTCGCCATTTCGCCGGAGCGGCTCGGCGGCCTGA
TCAAACTCATCAACGCGGGCGCGATCAGCAACACCATTGCCAAGCAGGTGTTCGAGATCATGCAGCAGGACGAGGCGACG
GCGGAAGCCATTGTCGAGCGCGAGGGGCTGGCGCAGGTTTCGGACAGGGGAGCCATCGAGGCGGCCATCAGGGAGATTCT
CGAAGCGAATCAGAAGCAGCTCGAACAGTACCGCAGCGGCAAAACGCAGCTTTTTGGTTTCTTCGTCGGCCAATGTATGC
AGAAGATGAAGGGCAAGGCGAACCCGAAAATGGTGAACGATATTCTGAGGTCGATGCTCGACGCCTGA

Upstream 100 bases:

>100_bases
GATGGGAGAACTCACTGTTGGCGCCCAGAAGTTCACTTTCAGGCAGTACCGGTGATTCGACCGGCATTCGACAAGGTTTT
TATCACTATCTCATTTTCTG

Downstream 100 bases:

>100_bases
GCAGGGTTGACTCGTCAGCTCTCCGTGCCGAACAGATCGTTCATGCGGGGGCTTTCGGGAGCGTTTTCAGGTTTGGCGGG
CCGGGCGGTCTCGGCGGGGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNYEIVVGLEVHCQLNTESKAFCGCSAKFGKPANTNVCPVCLALPGALPVLNARVVEDAVKLGLATNCTIARHSILARKN
YFYPDLPKGYQISQYEEPICSEGVIHIDLEEGGKDVRLVRIHIEEDAGKSIHDIGDDTYIDVNRCGVPLLEIVSYPDMRT
PKEASAYLQKLRQIVRYLGISDGNMEEGSLRCDANVSVRPVGATEYGTRTEIKNMNSFRNVERAIEYEAKRHIEVIEGGG
TIVQETRLWDADKLETRSMRGKEHAHDYRYFPDPDLVPVLVDDGMIRRMQEELPEFPEDRAARFVSEFGIPAYDAGVITV
DRELADYFESTVKVSGDAKASSNWVMGEVMRTLKEKYLDIHKFAISPERLGGLIKLINAGAISNTIAKQVFEIMQQDEAT
AEAIVEREGLAQVSDRGAIEAAIREILEANQKQLEQYRSGKTQLFGFFVGQCMQKMKGKANPKMVNDILRSMLDA

Sequences:

>Translated_475_residues
MNYEIVVGLEVHCQLNTESKAFCGCSAKFGKPANTNVCPVCLALPGALPVLNARVVEDAVKLGLATNCTIARHSILARKN
YFYPDLPKGYQISQYEEPICSEGVIHIDLEEGGKDVRLVRIHIEEDAGKSIHDIGDDTYIDVNRCGVPLLEIVSYPDMRT
PKEASAYLQKLRQIVRYLGISDGNMEEGSLRCDANVSVRPVGATEYGTRTEIKNMNSFRNVERAIEYEAKRHIEVIEGGG
TIVQETRLWDADKLETRSMRGKEHAHDYRYFPDPDLVPVLVDDGMIRRMQEELPEFPEDRAARFVSEFGIPAYDAGVITV
DRELADYFESTVKVSGDAKASSNWVMGEVMRTLKEKYLDIHKFAISPERLGGLIKLINAGAISNTIAKQVFEIMQQDEAT
AEAIVEREGLAQVSDRGAIEAAIREILEANQKQLEQYRSGKTQLFGFFVGQCMQKMKGKANPKMVNDILRSMLDA
>Mature_475_residues
MNYEIVVGLEVHCQLNTESKAFCGCSAKFGKPANTNVCPVCLALPGALPVLNARVVEDAVKLGLATNCTIARHSILARKN
YFYPDLPKGYQISQYEEPICSEGVIHIDLEEGGKDVRLVRIHIEEDAGKSIHDIGDDTYIDVNRCGVPLLEIVSYPDMRT
PKEASAYLQKLRQIVRYLGISDGNMEEGSLRCDANVSVRPVGATEYGTRTEIKNMNSFRNVERAIEYEAKRHIEVIEGGG
TIVQETRLWDADKLETRSMRGKEHAHDYRYFPDPDLVPVLVDDGMIRRMQEELPEFPEDRAARFVSEFGIPAYDAGVITV
DRELADYFESTVKVSGDAKASSNWVMGEVMRTLKEKYLDIHKFAISPERLGGLIKLINAGAISNTIAKQVFEIMQQDEAT
AEAIVEREGLAQVSDRGAIEAAIREILEANQKQLEQYRSGKTQLFGFFVGQCMQKMKGKANPKMVNDILRSMLDA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=36.1616161616162, Blast_Score=301, Evalue=7e-82,
Organism=Caenorhabditis elegans, GI115534094, Length=464, Percent_Identity=30.3879310344828, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6319391, Length=432, Percent_Identity=31.25, Blast_Score=209, Evalue=6e-55,
Organism=Drosophila melanogaster, GI45550808, Length=490, Percent_Identity=34.0816326530612, Blast_Score=292, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53092; Mature: 53092

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYEIVVGLEVHCQLNTESKAFCGCSAKFGKPANTNVCPVCLALPGALPVLNARVVEDAV
CCEEEEEEEEEEEEECCCCCCEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
KLGLATNCTIARHSILARKNYFYPDLPKGYQISQYEEPICSEGVIHIDLEEGGKDVRLVR
HHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEE
IHIEEDAGKSIHDIGDDTYIDVNRCGVPLLEIVSYPDMRTPKEASAYLQKLRQIVRYLGI
EEEECCCCCHHHHCCCCCEEEHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
SDGNMEEGSLRCDANVSVRPVGATEYGTRTEIKNMNSFRNVERAIEYEAKRHIEVIEGGG
CCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
TIVQETRLWDADKLETRSMRGKEHAHDYRYFPDPDLVPVLVDDGMIRRMQEELPEFPEDR
CEEECCCCCCCHHHHHHHHCCCHHCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHH
AARFVSEFGIPAYDAGVITVDRELADYFESTVKVSGDAKASSNWVMGEVMRTLKEKYLDI
HHHHHHHCCCCCCCCCEEEECHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHH
HKFAISPERLGGLIKLINAGAISNTIAKQVFEIMQQDEATAEAIVEREGLAQVSDRGAIE
HHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHH
AAIREILEANQKQLEQYRSGKTQLFGFFVGQCMQKMKGKANPKMVNDILRSMLDA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYEIVVGLEVHCQLNTESKAFCGCSAKFGKPANTNVCPVCLALPGALPVLNARVVEDAV
CCEEEEEEEEEEEEECCCCCCEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
KLGLATNCTIARHSILARKNYFYPDLPKGYQISQYEEPICSEGVIHIDLEEGGKDVRLVR
HHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEE
IHIEEDAGKSIHDIGDDTYIDVNRCGVPLLEIVSYPDMRTPKEASAYLQKLRQIVRYLGI
EEEECCCCCHHHHCCCCCEEEHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
SDGNMEEGSLRCDANVSVRPVGATEYGTRTEIKNMNSFRNVERAIEYEAKRHIEVIEGGG
CCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
TIVQETRLWDADKLETRSMRGKEHAHDYRYFPDPDLVPVLVDDGMIRRMQEELPEFPEDR
CEEECCCCCCCHHHHHHHHCCCHHCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHH
AARFVSEFGIPAYDAGVITVDRELADYFESTVKVSGDAKASSNWVMGEVMRTLKEKYLDI
HHHHHHHCCCCCCCCCEEEECHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHH
HKFAISPERLGGLIKLINAGAISNTIAKQVFEIMQQDEATAEAIVEREGLAQVSDRGAIE
HHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHH
AAIREILEANQKQLEQYRSGKTQLFGFFVGQCMQKMKGKANPKMVNDILRSMLDA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA