Click here to switch to the map view.

The map label for this gene is fusA-1 [H]

Identifier: 21675001

GI number: 21675001

Start: 2066057

End: 2068171

Strand: Reverse

Name: fusA-1 [H]

Synonym: CT2192

Alternate gene names: 21675001

Gene position: 2068171-2066057 (Counterclockwise)

Preceding gene: 21675002

Following gene: 21675000

Centisome position: 95.97

GC content: 54.23

Gene sequence:

>2115_bases
ATGGCACGGCAGGTTGCGTTAGATAGAGTCAGAAATATTGGTATCATGGCCCACATCGATGCGGGCAAGACCACGACGAC
AGAGAGGATTCTCTATTACACGGGTCGCCTGCACAAGATGGGCGAGGTGCATGAAGGTGGCGCCACCATGGACTGGATGG
AGCAGGAGAAGGAGCGTGGTATCACGATTACTTCCGCTGCGACAACCTGTTTTTGGACTCCGAAATACGGCAATTACGCC
GGTCTCAATCACAGGATCAATATTATTGATACTCCCGGCCACGTTGACTTCACGGTCGAGGTGGAGCGTTCCCTTCGTGT
GCTCGATGGAGCGGTTGCGCTATTCTGTGCTGTGGGCGGTGTCGAGCCCCAGTCTGAAACCGTCTGGCGTCAGGCTAACA
AGTATGGCGTTCCGAGGATCGCCTATGTTAACAAGATGGATCGCGTCGGCGCGAATTTCTTCGAGACTGTCAAGGCTATT
CGCGAGCGTCTCGGTGCCAACCCGGTACCGATTCAGATCCCGATCGGTCAGGGCGAGGTCTTCGCCGGCTTCGTCGATCT
GATCCGCATGAAGGGGATTATCTACGACAAGGAAGATGGTAGCACCTACACCGAAGTTGAAATTCCGCACGATCTTGAAA
ACGAGGCTCGCACCTGGCGCATCAACATGCTTGAGGCTGTTTCCGAGCTCGACGAGACCCTTCTTGAAAAATATCTTAAC
GGTGAGGACATTACTGAAGAGGAGATCCGCACCGTGCTTCGCCAGGCTACCCTGGGTGTGACTATCGTTCCGGTGCTCTG
CGGTTCGTCCTTCAAGAACAAGGGCGTGCAGTTCATGCTGGATGCCGTGATCGATTACCTGGCTTCGCCGGTCGATGATG
GTGAAGTGGAGGGGCATGATCCGAAAACTGAAGAACCGATCGTCCGTCAGCCGAAAGACGAGGAGCCGTTCGCTGCGCTT
GCTTTCAAGATTGCGACCGATCCGTTCGTCGGCAAGCTTACCTTCTTCCGGGTCTATTCAGGCGTGCTCAATGCAGGCAG
CTACGTGCTCAACTCGACTACCGGCAAAAAGGAGCGTGTTGGTCGCGTTTTGCAGATGCACTCCAACAAGCGCGAGGAGC
GTGATGCTGTGTATGCTGGCGATATCGCCGCTGCCGTTGGTCTCAAGGATGTGAGGACCGGTGATACGCTCTGCGACGAA
AGCAAGCCGATTGTACTTGAAAAGATGGTTTTCCCCGAACCGGTTATCGAGATTGCGGTTGAGCCAAAAACCAAGGCGGA
TAATGACAAACTCGGCATGTCTCTTGCCAAGCTTGCCGAAGAGGATCCGACCTTTAGGGTGAAAACTGATGAGGAAACCG
GTCAGACGCTTATCGCGGGTATGGGTGAGCTTCATCTCGAAATTCTGGTTGATCGTCTGAAGCGCGAATTCAAGGTCGAG
GCCAACGTTGGTCAGCCGCAGGTGGCCTATCGCGAAACCATTCGCGGTACGGTTGAATACGAAGGCAAGTTTGTTCGCCA
GTCTGGCGGTAAAGGTCAGTTCGGTCTGGTTGTGCTTAGGGTTGAGCCGCTCGAAGAGGGCAAAGGCTATGAGTTTGTCG
ATGAGATCAAGGGTGGCGTGATTCCGAAAGAGTATATCCCCGCAGTCAACGCAGGTATCCAGGAGGCAATGAAAGACGGT
GTTGTCGCCGGCTTTCCGATGCAGGATATCAAGGTTACACTGATCGACGGTAAGTACCACGAGGTTGACTCTTCGGAAAT
GGCATTCAAGATTGCAGGTTCAATCGGCTTCAAGGGTGCGGCCAAAAAGGCCAATCCGGTGCTGCTCGAACCGATTATGA
AGGTTGAGGTCATCACTCCGGAAGAGTATCTCGGCGATGTGATGGGTGATCTCTCGGGACGTCGCGGTCACATCGAAGGC
ATGGGTCAGCGTGCTGGTGCCCAGTTTGTCAGCGCAAAAGTGCCGCTGTCTCAAATGTTCGGCTACTCGACTGATCTCCG
ATCGATGACTCAGGGCCGCGCGAACTACTCGATGGAGTTCGAGAGCTATCGCGAGGTTCCTCGCAACATCGCCGAAGCAC
TGCAAGAGAAGAGGGTTGGCAAGGATTCCGAATAA

Upstream 100 bases:

>100_bases
GTGACGAGGTGCATCGCATGGCTGAGGCCAACAAGGCATTTGCTCACTTCAGGTTCTGAGTCTGTAGGCAAGAAAGAATT
ACTTAAAAAACGATCGATTT

Downstream 100 bases:

>100_bases
CAGTTTTTCATCACCGCATTACTAAACAGATAACCGACAGGGAGAAGAGTTATGGCTAAAGAGTCATACAAGAGGGATAA
ACCTCACGTAAATATTGGTA

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MARQVALDRVRNIGIMAHIDAGKTTTTERILYYTGRLHKMGEVHEGGATMDWMEQEKERGITITSAATTCFWTPKYGNYA
GLNHRINIIDTPGHVDFTVEVERSLRVLDGAVALFCAVGGVEPQSETVWRQANKYGVPRIAYVNKMDRVGANFFETVKAI
RERLGANPVPIQIPIGQGEVFAGFVDLIRMKGIIYDKEDGSTYTEVEIPHDLENEARTWRINMLEAVSELDETLLEKYLN
GEDITEEEIRTVLRQATLGVTIVPVLCGSSFKNKGVQFMLDAVIDYLASPVDDGEVEGHDPKTEEPIVRQPKDEEPFAAL
AFKIATDPFVGKLTFFRVYSGVLNAGSYVLNSTTGKKERVGRVLQMHSNKREERDAVYAGDIAAAVGLKDVRTGDTLCDE
SKPIVLEKMVFPEPVIEIAVEPKTKADNDKLGMSLAKLAEEDPTFRVKTDEETGQTLIAGMGELHLEILVDRLKREFKVE
ANVGQPQVAYRETIRGTVEYEGKFVRQSGGKGQFGLVVLRVEPLEEGKGYEFVDEIKGGVIPKEYIPAVNAGIQEAMKDG
VVAGFPMQDIKVTLIDGKYHEVDSSEMAFKIAGSIGFKGAAKKANPVLLEPIMKVEVITPEEYLGDVMGDLSGRRGHIEG
MGQRAGAQFVSAKVPLSQMFGYSTDLRSMTQGRANYSMEFESYREVPRNIAEALQEKRVGKDSE

Sequences:

>Translated_704_residues
MARQVALDRVRNIGIMAHIDAGKTTTTERILYYTGRLHKMGEVHEGGATMDWMEQEKERGITITSAATTCFWTPKYGNYA
GLNHRINIIDTPGHVDFTVEVERSLRVLDGAVALFCAVGGVEPQSETVWRQANKYGVPRIAYVNKMDRVGANFFETVKAI
RERLGANPVPIQIPIGQGEVFAGFVDLIRMKGIIYDKEDGSTYTEVEIPHDLENEARTWRINMLEAVSELDETLLEKYLN
GEDITEEEIRTVLRQATLGVTIVPVLCGSSFKNKGVQFMLDAVIDYLASPVDDGEVEGHDPKTEEPIVRQPKDEEPFAAL
AFKIATDPFVGKLTFFRVYSGVLNAGSYVLNSTTGKKERVGRVLQMHSNKREERDAVYAGDIAAAVGLKDVRTGDTLCDE
SKPIVLEKMVFPEPVIEIAVEPKTKADNDKLGMSLAKLAEEDPTFRVKTDEETGQTLIAGMGELHLEILVDRLKREFKVE
ANVGQPQVAYRETIRGTVEYEGKFVRQSGGKGQFGLVVLRVEPLEEGKGYEFVDEIKGGVIPKEYIPAVNAGIQEAMKDG
VVAGFPMQDIKVTLIDGKYHEVDSSEMAFKIAGSIGFKGAAKKANPVLLEPIMKVEVITPEEYLGDVMGDLSGRRGHIEG
MGQRAGAQFVSAKVPLSQMFGYSTDLRSMTQGRANYSMEFESYREVPRNIAEALQEKRVGKDSE
>Mature_703_residues
ARQVALDRVRNIGIMAHIDAGKTTTTERILYYTGRLHKMGEVHEGGATMDWMEQEKERGITITSAATTCFWTPKYGNYAG
LNHRINIIDTPGHVDFTVEVERSLRVLDGAVALFCAVGGVEPQSETVWRQANKYGVPRIAYVNKMDRVGANFFETVKAIR
ERLGANPVPIQIPIGQGEVFAGFVDLIRMKGIIYDKEDGSTYTEVEIPHDLENEARTWRINMLEAVSELDETLLEKYLNG
EDITEEEIRTVLRQATLGVTIVPVLCGSSFKNKGVQFMLDAVIDYLASPVDDGEVEGHDPKTEEPIVRQPKDEEPFAALA
FKIATDPFVGKLTFFRVYSGVLNAGSYVLNSTTGKKERVGRVLQMHSNKREERDAVYAGDIAAAVGLKDVRTGDTLCDES
KPIVLEKMVFPEPVIEIAVEPKTKADNDKLGMSLAKLAEEDPTFRVKTDEETGQTLIAGMGELHLEILVDRLKREFKVEA
NVGQPQVAYRETIRGTVEYEGKFVRQSGGKGQFGLVVLRVEPLEEGKGYEFVDEIKGGVIPKEYIPAVNAGIQEAMKDGV
VAGFPMQDIKVTLIDGKYHEVDSSEMAFKIAGSIGFKGAAKKANPVLLEPIMKVEVITPEEYLGDVMGDLSGRRGHIEGM
GQRAGAQFVSAKVPLSQMFGYSTDLRSMTQGRANYSMEFESYREVPRNIAEALQEKRVGKDSE

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=692, Percent_Identity=43.3526011560694, Blast_Score=539, Evalue=1e-153,
Organism=Homo sapiens, GI19923640, Length=733, Percent_Identity=38.7448840381992, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI25306287, Length=733, Percent_Identity=36.5620736698499, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI25306283, Length=452, Percent_Identity=41.1504424778761, Blast_Score=306, Evalue=4e-83,
Organism=Homo sapiens, GI4503483, Length=419, Percent_Identity=28.6396181384248, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI94966754, Length=142, Percent_Identity=38.0281690140845, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI157426893, Length=146, Percent_Identity=33.5616438356164, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI310132016, Length=123, Percent_Identity=36.5853658536585, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI310110807, Length=123, Percent_Identity=36.5853658536585, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI310123363, Length=123, Percent_Identity=36.5853658536585, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1789738, Length=704, Percent_Identity=64.4886363636364, Blast_Score=913, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=487, Percent_Identity=27.3100616016427, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI48994988, Length=145, Percent_Identity=40, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1788922, Length=146, Percent_Identity=36.3013698630137, Blast_Score=89, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=688, Percent_Identity=42.8779069767442, Blast_Score=534, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17556745, Length=729, Percent_Identity=28.2578875171468, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17506493, Length=496, Percent_Identity=27.0161290322581, Blast_Score=113, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=165, Percent_Identity=36.3636363636364, Blast_Score=96, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=144, Percent_Identity=31.9444444444444, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=144, Percent_Identity=31.9444444444444, Blast_Score=77, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=703, Percent_Identity=39.6870554765292, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6322359, Length=789, Percent_Identity=32.8263624841572, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324707, Length=800, Percent_Identity=25.875, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320593, Length=800, Percent_Identity=25.875, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323320, Length=164, Percent_Identity=35.3658536585366, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=147, Percent_Identity=36.0544217687075, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=693, Percent_Identity=44.7330447330447, Blast_Score=573, Evalue=1e-163,
Organism=Drosophila melanogaster, GI221458488, Length=730, Percent_Identity=31.2328767123288, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI24585709, Length=827, Percent_Identity=25.6348246674728, Blast_Score=183, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24585711, Length=827, Percent_Identity=25.6348246674728, Blast_Score=183, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24585713, Length=827, Percent_Identity=25.6348246674728, Blast_Score=183, Evalue=4e-46,
Organism=Drosophila melanogaster, GI78706572, Length=165, Percent_Identity=33.9393939393939, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI28574573, Length=143, Percent_Identity=34.2657342657343, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77953; Mature: 77822

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQVALDRVRNIGIMAHIDAGKTTTTERILYYTGRLHKMGEVHEGGATMDWMEQEKERG
CCCHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCHHHCCCCCCCCCCHHHHHHHHHCC
ITITSAATTCFWTPKYGNYAGLNHRINIIDTPGHVDFTVEVERSLRVLDGAVALFCAVGG
EEEEECCEEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
VEPQSETVWRQANKYGVPRIAYVNKMDRVGANFFETVKAIRERLGANPVPIQIPIGQGEV
CCCCHHHHHHHHHHCCCCEEHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEECCCCCH
FAGFVDLIRMKGIIYDKEDGSTYTEVEIPHDLENEARTWRINMLEAVSELDETLLEKYLN
HHHHHHHHHHCCEEEECCCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCC
GEDITEEEIRTVLRQATLGVTIVPVLCGSSFKNKGVQFMLDAVIDYLASPVDDGEVEGHD
CCCCCHHHHHHHHHHHHCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
PKTEEPIVRQPKDEEPFAALAFKIATDPFVGKLTFFRVYSGVLNAGSYVLNSTTGKKERV
CCCCCCCCCCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
GRVLQMHSNKREERDAVYAGDIAAAVGLKDVRTGDTLCDESKPIVLEKMVFPEPVIEIAV
HHHHHHHCCCCHHHCCEEECCHHHHHCCCCCCCCCCCCCCCCCEEEEHHCCCCCEEEEEE
EPKTKADNDKLGMSLAKLAEEDPTFRVKTDEETGQTLIAGMGELHLEILVDRLKREFKVE
CCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEE
ANVGQPQVAYRETIRGTVEYEGKFVRQSGGKGQFGLVVLRVEPLEEGKGYEFVDEIKGGV
CCCCCCHHHHHHHHCCEEEECCEEEECCCCCCCEEEEEEEEECCCCCCCCCHHHHHCCCC
IPKEYIPAVNAGIQEAMKDGVVAGFPMQDIKVTLIDGKYHEVDSSEMAFKIAGSIGFKGA
CCHHHHCHHHHHHHHHHHCCCEECCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCC
AKKANPVLLEPIMKVEVITPEEYLGDVMGDLSGRRGHIEGMGQRAGAQFVSAKVPLSQMF
CCCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHCHHHHCCCCCHHHHC
GYSTDLRSMTQGRANYSMEFESYREVPRNIAEALQEKRVGKDSE
CCCHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARQVALDRVRNIGIMAHIDAGKTTTTERILYYTGRLHKMGEVHEGGATMDWMEQEKERG
CCHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCHHHCCCCCCCCCCHHHHHHHHHCC
ITITSAATTCFWTPKYGNYAGLNHRINIIDTPGHVDFTVEVERSLRVLDGAVALFCAVGG
EEEEECCEEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
VEPQSETVWRQANKYGVPRIAYVNKMDRVGANFFETVKAIRERLGANPVPIQIPIGQGEV
CCCCHHHHHHHHHHCCCCEEHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEECCCCCH
FAGFVDLIRMKGIIYDKEDGSTYTEVEIPHDLENEARTWRINMLEAVSELDETLLEKYLN
HHHHHHHHHHCCEEEECCCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCC
GEDITEEEIRTVLRQATLGVTIVPVLCGSSFKNKGVQFMLDAVIDYLASPVDDGEVEGHD
CCCCCHHHHHHHHHHHHCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
PKTEEPIVRQPKDEEPFAALAFKIATDPFVGKLTFFRVYSGVLNAGSYVLNSTTGKKERV
CCCCCCCCCCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
GRVLQMHSNKREERDAVYAGDIAAAVGLKDVRTGDTLCDESKPIVLEKMVFPEPVIEIAV
HHHHHHHCCCCHHHCCEEECCHHHHHCCCCCCCCCCCCCCCCCEEEEHHCCCCCEEEEEE
EPKTKADNDKLGMSLAKLAEEDPTFRVKTDEETGQTLIAGMGELHLEILVDRLKREFKVE
CCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEE
ANVGQPQVAYRETIRGTVEYEGKFVRQSGGKGQFGLVVLRVEPLEEGKGYEFVDEIKGGV
CCCCCCHHHHHHHHCCEEEECCEEEECCCCCCCEEEEEEEEECCCCCCCCCHHHHHCCCC
IPKEYIPAVNAGIQEAMKDGVVAGFPMQDIKVTLIDGKYHEVDSSEMAFKIAGSIGFKGA
CCHHHHCHHHHHHHHHHHCCCEECCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCC
AKKANPVLLEPIMKVEVITPEEYLGDVMGDLSGRRGHIEGMGQRAGAQFVSAKVPLSQMF
CCCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHCHHHHCCCCCHHHHC
GYSTDLRSMTQGRANYSMEFESYREVPRNIAEALQEKRVGKDSE
CCCHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA