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The map label for this gene is tuf [H]

Identifier: 21675000

GI number: 21675000

Start: 2064824

End: 2066005

Strand: Reverse

Name: tuf [H]

Synonym: CT2191

Alternate gene names: 21675000

Gene position: 2066005-2064824 (Counterclockwise)

Preceding gene: 21675001

Following gene: 21674999

Centisome position: 95.87

GC content: 55.25

Gene sequence:

>1182_bases
ATGGCTAAAGAGTCATACAAGAGGGATAAACCTCACGTAAATATTGGTACAATCGGTCACGTTGACCACGGTAAAACCAC
TCTTACCGCTGCAATCACCAGCGTTCTCGCCAAGCAGGGCATGGCAACTTTACGTGAGTTCAGTGACATCGACAAAGCTC
CCGAAGAGAGAGAGCGCGGTATCACCATCTCGACGGCACACGTCGAGTATCAGACCGCAAAGCGTCACTACGCTCACATT
GATTGCCCTGGTCACGCTGACTATATCAAAAACATGATCACCGGTGCGGCCCAGATGGACGGCGCTATCCTCGTCGTTGC
CGGTACCGACGGCCCGATGCCCCAGACCCGTGAGCACATCCTGCTTGCCCGTCAGGTGAACGTTCCTGCGCTGGTCGTCT
TCCTGAACAAGGTTGACATCGCTGATCCGGAACTTCTTGAGCTGGTCGAGATGGAGCTTCGTGAGCTTCTTACCGAATAC
GGCTTCCCTGGCGACGATATTCCGATCATCAAAGGTTCGGCACTCAAGGCGCTCGAGGGGGATCCGGAGGCTGAGAAGCA
GATTATGGAACTCATGGACGCCGTTGACAGCTACATTCCGCAGCCTGTCCGTGACATTGACAAGCCGTTCCTAATGCCGG
TCGAAGACGTGTTCTCCATCTCTGGTCGCGGTACCGTCGGTACTGGTCGTATCGAGAGGGGCCGCATCAAGGTTGGCGAC
GAAGTCGAGATCGTCGGTATCAAGCCGACCGCAAAATCGGTTGTTACCGGTATTGAAATGTTCCAGAAGACCCTCGACGA
AGGTCAGGCAGGCGATAACGCAGGTCTGCTTCTCCGTGGTGTTGACAAGAATGCGCTCGAGCGCGGCATGGTTATCGCCA
AGCCGGGTTCGATTACCCCACACACCAAGTTCAAGGCTGAGGTTTACATCCTGAAGAAGGAAGAGGGTGGCCGTCACACT
CCGTTCTTCAACGGCTATCGTCCGCAGTTTTACTTCAGAACCACCGACGTTACCGGTTCGGTGACCCTTCCTGAGGGCGT
CGAAATGGTTATGCCTGGTGACAACCTCTCCATCGATGTCGAGCTGATCGCTCCTATCGCCATGGAAGAGAGCCTCAGGT
TCGCAATCCGCGAAGGCGGCCGTACGGTTGGTGCCGGTTCGGTAACCAAAATCGTCGAGTGA

Upstream 100 bases:

>100_bases
CATCGCCGAAGCACTGCAAGAGAAGAGGGTTGGCAAGGATTCCGAATAACAGTTTTTCATCACCGCATTACTAAACAGAT
AACCGACAGGGAGAAGAGTT

Downstream 100 bases:

>100_bases
TCGGATTGATCGTTATCCCGGGGCGAGGCAGCGCTCTCGCCCCTTTTTTATTGAAACCATCGAAACAGGGATAGACCAGT
GGCTGTTCAGCAAAAGATCA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MAKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKQGMATLREFSDIDKAPEERERGITISTAHVEYQTAKRHYAHI
DCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHILLARQVNVPALVVFLNKVDIADPELLELVEMELRELLTEY
GFPGDDIPIIKGSALKALEGDPEAEKQIMELMDAVDSYIPQPVRDIDKPFLMPVEDVFSISGRGTVGTGRIERGRIKVGD
EVEIVGIKPTAKSVVTGIEMFQKTLDEGQAGDNAGLLLRGVDKNALERGMVIAKPGSITPHTKFKAEVYILKKEEGGRHT
PFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSIDVELIAPIAMEESLRFAIREGGRTVGAGSVTKIVE

Sequences:

>Translated_393_residues
MAKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKQGMATLREFSDIDKAPEERERGITISTAHVEYQTAKRHYAHI
DCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHILLARQVNVPALVVFLNKVDIADPELLELVEMELRELLTEY
GFPGDDIPIIKGSALKALEGDPEAEKQIMELMDAVDSYIPQPVRDIDKPFLMPVEDVFSISGRGTVGTGRIERGRIKVGD
EVEIVGIKPTAKSVVTGIEMFQKTLDEGQAGDNAGLLLRGVDKNALERGMVIAKPGSITPHTKFKAEVYILKKEEGGRHT
PFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSIDVELIAPIAMEESLRFAIREGGRTVGAGSVTKIVE
>Mature_392_residues
AKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKQGMATLREFSDIDKAPEERERGITISTAHVEYQTAKRHYAHID
CPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHILLARQVNVPALVVFLNKVDIADPELLELVEMELRELLTEYG
FPGDDIPIIKGSALKALEGDPEAEKQIMELMDAVDSYIPQPVRDIDKPFLMPVEDVFSISGRGTVGTGRIERGRIKVGDE
VEIVGIKPTAKSVVTGIEMFQKTLDEGQAGDNAGLLLRGVDKNALERGMVIAKPGSITPHTKFKAEVYILKKEEGGRHTP
FFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSIDVELIAPIAMEESLRFAIREGGRTVGAGSVTKIVE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.6294416243655, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=31.651376146789, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=31.651376146789, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=28.9237668161435, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=29.1479820627803, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI54607086, Length=346, Percent_Identity=32.9479768786127, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI194018522, Length=444, Percent_Identity=26.1261261261261, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194097354, Length=444, Percent_Identity=26.1261261261261, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194018520, Length=444, Percent_Identity=26.1261261261261, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI46094014, Length=443, Percent_Identity=26.6365688487585, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4503507, Length=278, Percent_Identity=27.6978417266187, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI157426893, Length=238, Percent_Identity=28.1512605042017, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=35.0746268656716, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=71.7557251908397, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=71.7557251908397, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=381, Percent_Identity=30.1837270341207, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=33.6099585062241, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1789108, Length=328, Percent_Identity=29.5731707317073, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788922, Length=140, Percent_Identity=35, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789738, Length=151, Percent_Identity=34.4370860927152, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.1328320802005, Blast_Score=403, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=370, Percent_Identity=46.4864864864865, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=452, Percent_Identity=31.1946902654867, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=452, Percent_Identity=31.1946902654867, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=429, Percent_Identity=31.002331002331, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=29.0909090909091, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI115532065, Length=440, Percent_Identity=29.0909090909091, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32566629, Length=442, Percent_Identity=26.6968325791855, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=30.5369127516779, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=41.4965986394558, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17509919, Length=281, Percent_Identity=29.1814946619217, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=30.6382978723404, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556745, Length=133, Percent_Identity=34.5864661654135, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=67.5062972292191, Blast_Score=536, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=29.2792792792793, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=29.2792792792793, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=454, Percent_Identity=24.4493392070485, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6320863, Length=285, Percent_Identity=29.8245614035088, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320377, Length=344, Percent_Identity=28.1976744186047, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=29.8319327731092, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.0740740740741, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=58.8688946015424, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=58.8688946015424, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=48.0719794344473, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=30.2690582959641, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=30.2690582959641, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=30.6487695749441, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=30.6487695749441, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=30.6487695749441, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=30.6487695749441, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=32.8947368421053, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45550900, Length=447, Percent_Identity=27.9642058165548, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17137380, Length=449, Percent_Identity=25.6124721603563, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=28.9285714285714, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=28.9285714285714, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI78706572, Length=249, Percent_Identity=26.9076305220884, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=35.7575757575758, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI28574573, Length=140, Percent_Identity=36.4285714285714, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42900; Mature: 42769

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKQGMATLREFSDIDKAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
ITISTAHVEYQTAKRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHI
CEEEEEEEEEHHHHCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVNVPALVVFLNKVDIADPELLELVEMELRELLTEYGFPGDDIPIIKGSALKALEG
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHCCC
DPEAEKQIMELMDAVDSYIPQPVRDIDKPFLMPVEDVFSISGRGTVGTGRIERGRIKVGD
CCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCCCCCCCEECCEEEECC
EVEIVGIKPTAKSVVTGIEMFQKTLDEGQAGDNAGLLLRGVDKNALERGMVIAKPGSITP
EEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHCCEEEECCCCCCC
HTKFKAEVYILKKEEGGRHTPFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSIDV
CCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCCEEEE
ELIAPIAMEESLRFAIREGGRTVGAGSVTKIVE
EEECCHHHHHHHHHHHHCCCCEECCCCHHHHCC
>Mature Secondary Structure 
AKESYKRDKPHVNIGTIGHVDHGKTTLTAAITSVLAKQGMATLREFSDIDKAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
ITISTAHVEYQTAKRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAGTDGPMPQTREHI
CEEEEEEEEEHHHHCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVNVPALVVFLNKVDIADPELLELVEMELRELLTEYGFPGDDIPIIKGSALKALEG
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHCCC
DPEAEKQIMELMDAVDSYIPQPVRDIDKPFLMPVEDVFSISGRGTVGTGRIERGRIKVGD
CCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCCCCCCCEECCEEEECC
EVEIVGIKPTAKSVVTGIEMFQKTLDEGQAGDNAGLLLRGVDKNALERGMVIAKPGSITP
EEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHCCEEEECCCCCCC
HTKFKAEVYILKKEEGGRHTPFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLSIDV
CCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCCEEEE
ELIAPIAMEESLRFAIREGGRTVGAGSVTKIVE
EEECCHHHHHHHHHHHHCCCCEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8085791 [H]