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The map label for this gene is purA [H]

Identifier: 21674963

GI number: 21674963

Start: 2041807

End: 2043114

Strand: Reverse

Name: purA [H]

Synonym: CT2154

Alternate gene names: 21674963

Gene position: 2043114-2041807 (Counterclockwise)

Preceding gene: 21674964

Following gene: 21674962

Centisome position: 94.81

GC content: 59.94

Gene sequence:

>1308_bases
GTGGAAGAAAAAATATTCAGCAGGCCGGCAGCGTCTGCTACCGTGCTTGTGGGCACGCAGTTCGGCGACGAGGGCAAAGG
CAAGCTGGTCGATTACCTTTCGGACAAATACGACATCGTGGTGCGTTACCAGGGCGGTGCCAACGCCGGTCACACCATCT
GCTTCGACGGCAAAACCGTCGTGCTCCACCTCATCCCCTCGGGCATCTTCAACAAGGATTGCATCTGCGTGATCGGCAAC
GGCGTCGTGATCGATCCGAACGCGCTCATGGATGAGATCAAAAAGGTTGAGGAGCTGGGCTACGACGTCAAGGGGCGTCT
CTACATCAGCCACAACGCGCACCTGATCATGCCGTACCACAAGCGGCTCGATTCGCTGAGCGAGTCCTGCCTGAGTGGCG
ATAACAAGATTGGCACCACCGGTCGTGGTATCGGCCCGAGCTACGAGGATAAGTTCGCCCGCAAAGGCATCCGGGTGGTC
GATCTGCTCGATCGCGACGTGCTGAAGGAGAAGCTGCGCGAGAACCTCGCGGCCAAGAACAAGCTGATCAGCAAGGTGTA
CGAGCAGGAGGAGATCGACGTCGAGGCGATCATCCGCGAGTACGAGGAGTTCGACAAGGCGATCGATCCCTACGTGACCA
ACACCCAGCTCTTCCTGAATCGCCAGATCAAGGCGGGCAAGACCATCCTGCTCGAAGGCGCGCAGGGTTGCCTGCTCGAT
GTCGATCACGGCACCTATCCCTTCGTGACCTCCTCCAACCCGACCTCCGGCGGCGCTTGCACCGGTTCCGGTGTGGCTCC
GAACCACGTCGGCAAGATCATCGGCGTCTGCAAGGCCTACACGACAAGGGTGGGCAACGGCGATTTCCCGACCGAGCTGG
ATGACGAGACTGGCGAAGCGCTTGGTCGCATCGGCTGCGAGTTTGGCGCGACCACGGGGCGTAAGCGTCGCTGCGGCTGG
CTCGATCTCGTCGCCCTGCGCTATTCGGTTACCGTCAGCGGCGTGACCGAGCTGGCGCTGACCAAGCTCGACGTGCTCGA
CACCTTCGAGGAGATCAAGGTGTGCACCTCATACATGCTCGACGGTAAGGAAATTTTCGACTTCCCGACCGAGCACCAGA
CCCTGTCGCGAGTGCAGCCGGTTTACAAGTCGCTCAAGGGCTGGATGGCTTCGAATGCCAAGGCAAAGAGCTTCGCGGAG
ATGCATCCCAACGCTCAGGCATACGTCAACTTCCTCGAAGAGGCTCTGGAGGTTCCGGTGACCTTCATCTCTGTCGGACC
GGGCAGGGACGAGACGGTCTTCAAGTAA

Upstream 100 bases:

>100_bases
CGAATGCCAGATTTATTGTAAATTATTCGTCTTTGTCGGGCAGTGGCCGGGACAGGCTTCTGGCTTCGATCGATACTTTT
ACTCAGTAAAAACAAGCGAC

Downstream 100 bases:

>100_bases
GAAATTGGGCTGAAGCCCGGATTTTTTTGCTCCGTAACCCCGGCGTGAAAGCCGGGGCAACATGAATGCAAGAGGGGATG
AATGTCCGTCGGGCTGGACC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MEEKIFSRPAASATVLVGTQFGDEGKGKLVDYLSDKYDIVVRYQGGANAGHTICFDGKTVVLHLIPSGIFNKDCICVIGN
GVVIDPNALMDEIKKVEELGYDVKGRLYISHNAHLIMPYHKRLDSLSESCLSGDNKIGTTGRGIGPSYEDKFARKGIRVV
DLLDRDVLKEKLRENLAAKNKLISKVYEQEEIDVEAIIREYEEFDKAIDPYVTNTQLFLNRQIKAGKTILLEGAQGCLLD
VDHGTYPFVTSSNPTSGGACTGSGVAPNHVGKIIGVCKAYTTRVGNGDFPTELDDETGEALGRIGCEFGATTGRKRRCGW
LDLVALRYSVTVSGVTELALTKLDVLDTFEEIKVCTSYMLDGKEIFDFPTEHQTLSRVQPVYKSLKGWMASNAKAKSFAE
MHPNAQAYVNFLEEALEVPVTFISVGPGRDETVFK

Sequences:

>Translated_435_residues
MEEKIFSRPAASATVLVGTQFGDEGKGKLVDYLSDKYDIVVRYQGGANAGHTICFDGKTVVLHLIPSGIFNKDCICVIGN
GVVIDPNALMDEIKKVEELGYDVKGRLYISHNAHLIMPYHKRLDSLSESCLSGDNKIGTTGRGIGPSYEDKFARKGIRVV
DLLDRDVLKEKLRENLAAKNKLISKVYEQEEIDVEAIIREYEEFDKAIDPYVTNTQLFLNRQIKAGKTILLEGAQGCLLD
VDHGTYPFVTSSNPTSGGACTGSGVAPNHVGKIIGVCKAYTTRVGNGDFPTELDDETGEALGRIGCEFGATTGRKRRCGW
LDLVALRYSVTVSGVTELALTKLDVLDTFEEIKVCTSYMLDGKEIFDFPTEHQTLSRVQPVYKSLKGWMASNAKAKSFAE
MHPNAQAYVNFLEEALEVPVTFISVGPGRDETVFK
>Mature_435_residues
MEEKIFSRPAASATVLVGTQFGDEGKGKLVDYLSDKYDIVVRYQGGANAGHTICFDGKTVVLHLIPSGIFNKDCICVIGN
GVVIDPNALMDEIKKVEELGYDVKGRLYISHNAHLIMPYHKRLDSLSESCLSGDNKIGTTGRGIGPSYEDKFARKGIRVV
DLLDRDVLKEKLRENLAAKNKLISKVYEQEEIDVEAIIREYEEFDKAIDPYVTNTQLFLNRQIKAGKTILLEGAQGCLLD
VDHGTYPFVTSSNPTSGGACTGSGVAPNHVGKIIGVCKAYTTRVGNGDFPTELDDETGEALGRIGCEFGATTGRKRRCGW
LDLVALRYSVTVSGVTELALTKLDVLDTFEEIKVCTSYMLDGKEIFDFPTEHQTLSRVQPVYKSLKGWMASNAKAKSFAE
MHPNAQAYVNFLEEALEVPVTFISVGPGRDETVFK

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=436, Percent_Identity=47.2477064220183, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI22748717, Length=430, Percent_Identity=46.5116279069767, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=45.959595959596, Blast_Score=331, Evalue=1e-90,
Organism=Escherichia coli, GI1790620, Length=421, Percent_Identity=47.2684085510689, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=43.9814814814815, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=43.9814814814815, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=45.3703703703704, Blast_Score=373, Evalue=1e-104,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=45.9715639810426, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47774; Mature: 47774

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKIFSRPAASATVLVGTQFGDEGKGKLVDYLSDKYDIVVRYQGGANAGHTICFDGKTV
CCCCHHCCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEEECCCEE
VLHLIPSGIFNKDCICVIGNGVVIDPNALMDEIKKVEELGYDVKGRLYISHNAHLIMPYH
EEEEECCCCCCCCEEEEECCCEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHH
KRLDSLSESCLSGDNKIGTTGRGIGPSYEDKFARKGIRVVDLLDRDVLKEKLRENLAAKN
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KLISKVYEQEEIDVEAIIREYEEFDKAIDPYVTNTQLFLNRQIKAGKTILLEGAQGCLLD
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCEEE
VDHGTYPFVTSSNPTSGGACTGSGVAPNHVGKIIGVCKAYTTRVGNGDFPTELDDETGEA
CCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
LGRIGCEFGATTGRKRRCGWLDLVALRYSVTVSGVTELALTKLDVLDTFEEIKVCTSYML
HHHHCCCCCCCCCCCCCCCHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGKEIFDFPTEHQTLSRVQPVYKSLKGWMASNAKAKSFAEMHPNAQAYVNFLEEALEVPV
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCE
TFISVGPGRDETVFK
EEEEECCCCCCCCCC
>Mature Secondary Structure
MEEKIFSRPAASATVLVGTQFGDEGKGKLVDYLSDKYDIVVRYQGGANAGHTICFDGKTV
CCCCHHCCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEEECCCEE
VLHLIPSGIFNKDCICVIGNGVVIDPNALMDEIKKVEELGYDVKGRLYISHNAHLIMPYH
EEEEECCCCCCCCEEEEECCCEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHH
KRLDSLSESCLSGDNKIGTTGRGIGPSYEDKFARKGIRVVDLLDRDVLKEKLRENLAAKN
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KLISKVYEQEEIDVEAIIREYEEFDKAIDPYVTNTQLFLNRQIKAGKTILLEGAQGCLLD
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCEEE
VDHGTYPFVTSSNPTSGGACTGSGVAPNHVGKIIGVCKAYTTRVGNGDFPTELDDETGEA
CCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
LGRIGCEFGATTGRKRRCGWLDLVALRYSVTVSGVTELALTKLDVLDTFEEIKVCTSYML
HHHHCCCCCCCCCCCCCCCHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGKEIFDFPTEHQTLSRVQPVYKSLKGWMASNAKAKSFAEMHPNAQAYVNFLEEALEVPV
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCE
TFISVGPGRDETVFK
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA