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The map label for this gene is tig

Identifier: 21674746

GI number: 21674746

Start: 1828293

End: 1829573

Strand: Direct

Name: tig

Synonym: CT1934

Alternate gene names: 21674746

Gene position: 1828293-1829573 (Clockwise)

Preceding gene: 21674744

Following gene: 21674749

Centisome position: 84.84

GC content: 52.77

Gene sequence:

>1281_bases
TTGCAAAAGAATATCACGAATGTCAGCGAAATCGCGCAGGAACTTGAAATTATTCTGACTGCCGAAGAGTACCAGCCCGA
ATATGACCAGCAGCTCGAAGAAGCGCGCAAGTCCGTCAGAATTAAAGGCTTCAGGCAGGGGCACGTGCCCGTGGGCATGC
TCAAGCGCATTATCGGACCCTCCATCGAGGCCGAGGTTGCCGAGAAGATGGCCTCGAAGTATTTCGCAGCCATCGCCGAG
GAGGAGAAGATCAACCCAGCCAGCCGCGCCCAGATCGAGAGCTACAACTACGAGGATGGCAAACTGACCATCAAAATTTC
TTACGAAATCCACCCCGAATTCGAGTTGAAGGATTTCAGCGAATACACCTTCACACAGGCAGAATACACCATCTCCGACG
AGGATGTCGATCGTGAAATCAAACTGATCCTGCGTGGTCACGGCACCATGGTAACCAGCGAAGATGCTGCAGCCGAGGGC
GACACGGTCATCGGCGACGTTACGAAGCTTGACGCCGATGGTGCTGACATTGAAGGCTCGAAGAATGAAAACCACCATTT
CAACCTCGAATACCTCCCGGCTGACAACCCGTTCCGCATGGCCCTCGAAGGCAAGAAAGCCGGGGATGTGGTCGATGTCA
CCGTCAAGCCAAAGGAAGAGGGCGGCGAAACGAACCGCTTCCGCATCGAAATCAAAGAGGTCAAACACCTCGAACTGCCC
GAACTGGACGACGAACTGGTCAAGGAGATCAGCCAGCAGCGCTTCGAGAAGGTCGAGGACTTCAGAAACGACATCCGTTT
GCAGCTCCAGGCACATTTCTCCGACAAATCCGAGTATGATCTGCTCGAAGCCATTTCGTCAAAGCTTATCGAAGAGCATC
CGGTGCCCACGCCAAGCGCCATGGTGGCCCATTTCCAAAATATACTGCTCGAAAACGCCAAGCGCCAGGTTGGCGGCCAG
TTCCCTAAAGGCTTTGACGAGAGAGAGTTCTTCAACGCCATGAAGCCGAATGCTGAAAAGCACGCCCGCTGGCTTCTGAT
CAGCCAGAAGATCGCCAAAGAGAACAATCTTGAGGTAACTGACGAGGATATCAAGGCATTTGCCGAAAAAGAGGCAGAAA
AGGAACCGTCACTGACTGTGGATCAGCTTCTCAACACCTACCTGTCCACCGAGTTCAAGGACTACATCATCGACACGATC
CTGAAAGAGAAAATCTACGATGTGATCAAATCGAAGGTGACCATCACCAAAGAAGCTACACCGGTTCCGGCGCACAACTG
A

Upstream 100 bases:

>100_bases
ATTCAGGCTTTAAAATTTCAGGGATACTCTCTGTACCGATCCACGCTTTCCGGCTCACCGGAAACGGAGAGACCCGCAAC
TTAACCGGTCACGAAAAGCC

Downstream 100 bases:

>100_bases
AGTAATCGAAAAATAAGGCTTTCTGAAGCAAAAAGGCTCATCGACTGATGAGCCTTTTTTTATGCGCGACGGGGAAATTG
CCGGATGCTCAGCGCGGCTC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MQKNITNVSEIAQELEIILTAEEYQPEYDQQLEEARKSVRIKGFRQGHVPVGMLKRIIGPSIEAEVAEKMASKYFAAIAE
EEKINPASRAQIESYNYEDGKLTIKISYEIHPEFELKDFSEYTFTQAEYTISDEDVDREIKLILRGHGTMVTSEDAAAEG
DTVIGDVTKLDADGADIEGSKNENHHFNLEYLPADNPFRMALEGKKAGDVVDVTVKPKEEGGETNRFRIEIKEVKHLELP
ELDDELVKEISQQRFEKVEDFRNDIRLQLQAHFSDKSEYDLLEAISSKLIEEHPVPTPSAMVAHFQNILLENAKRQVGGQ
FPKGFDEREFFNAMKPNAEKHARWLLISQKIAKENNLEVTDEDIKAFAEKEAEKEPSLTVDQLLNTYLSTEFKDYIIDTI
LKEKIYDVIKSKVTITKEATPVPAHN

Sequences:

>Translated_426_residues
MQKNITNVSEIAQELEIILTAEEYQPEYDQQLEEARKSVRIKGFRQGHVPVGMLKRIIGPSIEAEVAEKMASKYFAAIAE
EEKINPASRAQIESYNYEDGKLTIKISYEIHPEFELKDFSEYTFTQAEYTISDEDVDREIKLILRGHGTMVTSEDAAAEG
DTVIGDVTKLDADGADIEGSKNENHHFNLEYLPADNPFRMALEGKKAGDVVDVTVKPKEEGGETNRFRIEIKEVKHLELP
ELDDELVKEISQQRFEKVEDFRNDIRLQLQAHFSDKSEYDLLEAISSKLIEEHPVPTPSAMVAHFQNILLENAKRQVGGQ
FPKGFDEREFFNAMKPNAEKHARWLLISQKIAKENNLEVTDEDIKAFAEKEAEKEPSLTVDQLLNTYLSTEFKDYIIDTI
LKEKIYDVIKSKVTITKEATPVPAHN
>Mature_426_residues
MQKNITNVSEIAQELEIILTAEEYQPEYDQQLEEARKSVRIKGFRQGHVPVGMLKRIIGPSIEAEVAEKMASKYFAAIAE
EEKINPASRAQIESYNYEDGKLTIKISYEIHPEFELKDFSEYTFTQAEYTISDEDVDREIKLILRGHGTMVTSEDAAAEG
DTVIGDVTKLDADGADIEGSKNENHHFNLEYLPADNPFRMALEGKKAGDVVDVTVKPKEEGGETNRFRIEIKEVKHLELP
ELDDELVKEISQQRFEKVEDFRNDIRLQLQAHFSDKSEYDLLEAISSKLIEEHPVPTPSAMVAHFQNILLENAKRQVGGQ
FPKGFDEREFFNAMKPNAEKHARWLLISQKIAKENNLEVTDEDIKAFAEKEAEKEPSLTVDQLLNTYLSTEFKDYIIDTI
LKEKIYDVIKSKVTITKEATPVPAHN

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=432, Percent_Identity=23.6111111111111, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_CHLTE (Q8KB57)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662811.1
- ProteinModelPortal:   Q8KB57
- SMR:   Q8KB57
- GeneID:   1008269
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1934
- NMPDR:   fig|194439.1.peg.1905
- TIGR:   CT1934
- HOGENOM:   HBG405050
- OMA:   YIKEKAF
- ProtClustDB:   CLSK638004
- BioCyc:   CTEP194439:CT_1934-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48609; Mature: 48609

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKNITNVSEIAQELEIILTAEEYQPEYDQQLEEARKSVRIKGFRQGHVPVGMLKRIIGP
CCCCCCCHHHHHHHHHEEEEEHHHCCHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHCC
SIEAEVAEKMASKYFAAIAEEEKINPASRAQIESYNYEDGKLTIKISYEIHPEFELKDFS
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCC
EYTFTQAEYTISDEDVDREIKLILRGHGTMVTSEDAAAEGDTVIGDVTKLDADGADIEGS
CCEEECEEEEECCCCCCHHEEEEEECCCCEEECCCCCCCCCEEEECCEEECCCCCCCCCC
KNENHHFNLEYLPADNPFRMALEGKKAGDVVDVTVKPKEEGGETNRFRIEIKEVKHLELP
CCCCCEEEEEEECCCCCEEEEECCCCCCCEEEEEECCHHCCCCCCEEEEEEECCCCCCCC
ELDDELVKEISQQRFEKVEDFRNDIRLQLQAHFSDKSEYDLLEAISSKLIEEHPVPTPSA
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHH
MVAHFQNILLENAKRQVGGQFPKGFDEREFFNAMKPNAEKHARWLLISQKIAKENNLEVT
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEC
DEDIKAFAEKEAEKEPSLTVDQLLNTYLSTEFKDYIIDTILKEKIYDVIKSKVTITKEAT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
PVPAHN
CCCCCC
>Mature Secondary Structure
MQKNITNVSEIAQELEIILTAEEYQPEYDQQLEEARKSVRIKGFRQGHVPVGMLKRIIGP
CCCCCCCHHHHHHHHHEEEEEHHHCCHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHCC
SIEAEVAEKMASKYFAAIAEEEKINPASRAQIESYNYEDGKLTIKISYEIHPEFELKDFS
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCC
EYTFTQAEYTISDEDVDREIKLILRGHGTMVTSEDAAAEGDTVIGDVTKLDADGADIEGS
CCEEECEEEEECCCCCCHHEEEEEECCCCEEECCCCCCCCCEEEECCEEECCCCCCCCCC
KNENHHFNLEYLPADNPFRMALEGKKAGDVVDVTVKPKEEGGETNRFRIEIKEVKHLELP
CCCCCEEEEEEECCCCCEEEEECCCCCCCEEEEEECCHHCCCCCCEEEEEEECCCCCCCC
ELDDELVKEISQQRFEKVEDFRNDIRLQLQAHFSDKSEYDLLEAISSKLIEEHPVPTPSA
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHH
MVAHFQNILLENAKRQVGGQFPKGFDEREFFNAMKPNAEKHARWLLISQKIAKENNLEVT
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEC
DEDIKAFAEKEAEKEPSLTVDQLLNTYLSTEFKDYIIDTILKEKIYDVIKSKVTITKEAT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
PVPAHN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901