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The map label for this gene is hypB [H]

Identifier: 21674612

GI number: 21674612

Start: 1702969

End: 1703808

Strand: Reverse

Name: hypB [H]

Synonym: CT1798

Alternate gene names: 21674612

Gene position: 1703808-1702969 (Counterclockwise)

Preceding gene: 21674613

Following gene: 21674611

Centisome position: 79.06

GC content: 61.79

Gene sequence:

>840_bases
ATGTGCGATACTTGCGGTTGTTCAGGCGATGGCGGAGCGGTGCTGCGCAAGCCGGGCGTCAAAGATTATCATGTCCACGT
CGGCGATGACGGCCATCACCATCACCACGACCACCAACACAGTCATGACCACGGACACAGCCACGATCATGCGCATGAGC
ACCATCACGATCACCACCATGGCGAGGCTCGCAAGGTGCAGATGGAGCAGGATGTGCTGTTGCAAAACAACATGCTTGCC
GAGCGCAACCGTGGCTGGTTCGAGGCGCGGCGGGTGCTGGCGCTGAACTTTCTCAGTTCGCCCGGCTCAGGCAAGACCTC
GATTCTCGAAAAAACCATCCCGGCGCTGCTGGAGCAGTGCCCCGTGACGGTGATCGAGGGCGACCAGCAGAGCACCAACG
ACGCCGACCGGATCGACGCGCTCGGCGTGCCGGTGATCCAGGTCAACACCGGCACCGGCTGCCACCTCGACGCGCAGATG
GTGCAGCGCGCCGTGCGTGAACTCGATCCGCCGGAGCGCTCGCTCGTCTGCATCGAGAACGTCGGCAACCTGGTCTGCCC
GGCGCTTTTCGACCTCGGTGAAGCGGCCAAGGTGGTGGTCATCAGCGTGACCGAGGGGGACGACAAGCCGCTCAAATATC
CCACCATGTTCCACGAGGCGGACATCTGCCTGCTCAACAAGACCGACCTGTTGCCGTACGTCGATTTCGACGTGGCCAAA
TGCCGCGAGTACGCCATGCAGGTCAATCACCATCTCGAATGGATCGAGGTTTCTGCCAAGACCGGCGAGGGCTTCGACCA
GTGGATTGCATGGCTGAAAACGAAGCTCGCGGCGCTCTGA

Upstream 100 bases:

>100_bases
ATCGAATCTGGCCGGGAGCTTGCGGTGCGTTCATTCACGATTGAGTGAAAACGGCTGCTCAACCATACTTGTAATTATAC
CATTCTGGGAGTAATCAACT

Downstream 100 bases:

>100_bases
GCGGTTTGTCTCAACGTGAGCGCCGCCGGATCGAGGTTCGCGGCATCGTGCAGGGGGTGGGCTTCCGTCCCTTCGTCTGG
CGGCTTGCTCATCAGCTCGA

Product: hydrogenase accessory protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MCDTCGCSGDGGAVLRKPGVKDYHVHVGDDGHHHHHDHQHSHDHGHSHDHAHEHHHDHHHGEARKVQMEQDVLLQNNMLA
ERNRGWFEARRVLALNFLSSPGSGKTSILEKTIPALLEQCPVTVIEGDQQSTNDADRIDALGVPVIQVNTGTGCHLDAQM
VQRAVRELDPPERSLVCIENVGNLVCPALFDLGEAAKVVVISVTEGDDKPLKYPTMFHEADICLLNKTDLLPYVDFDVAK
CREYAMQVNHHLEWIEVSAKTGEGFDQWIAWLKTKLAAL

Sequences:

>Translated_279_residues
MCDTCGCSGDGGAVLRKPGVKDYHVHVGDDGHHHHHDHQHSHDHGHSHDHAHEHHHDHHHGEARKVQMEQDVLLQNNMLA
ERNRGWFEARRVLALNFLSSPGSGKTSILEKTIPALLEQCPVTVIEGDQQSTNDADRIDALGVPVIQVNTGTGCHLDAQM
VQRAVRELDPPERSLVCIENVGNLVCPALFDLGEAAKVVVISVTEGDDKPLKYPTMFHEADICLLNKTDLLPYVDFDVAK
CREYAMQVNHHLEWIEVSAKTGEGFDQWIAWLKTKLAAL
>Mature_279_residues
MCDTCGCSGDGGAVLRKPGVKDYHVHVGDDGHHHHHDHQHSHDHGHSHDHAHEHHHDHHHGEARKVQMEQDVLLQNNMLA
ERNRGWFEARRVLALNFLSSPGSGKTSILEKTIPALLEQCPVTVIEGDQQSTNDADRIDALGVPVIQVNTGTGCHLDAQM
VQRAVRELDPPERSLVCIENVGNLVCPALFDLGEAAKVVVISVTEGDDKPLKYPTMFHEADICLLNKTDLLPYVDFDVAK
CREYAMQVNHHLEWIEVSAKTGEGFDQWIAWLKTKLAAL

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=297, Percent_Identity=47.8114478114478, Blast_Score=261, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 31074; Mature: 31074

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCDTCGCSGDGGAVLRKPGVKDYHVHVGDDGHHHHHDHQHSHDHGHSHDHAHEHHHDHHH
CCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCC
GEARKVQMEQDVLLQNNMLAERNRGWFEARRVLALNFLSSPGSGKTSILEKTIPALLEQC
CCHHEEEHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
PVTVIEGDQQSTNDADRIDALGVPVIQVNTGTGCHLDAQMVQRAVRELDPPERSLVCIEN
CEEEEECCCCCCCCHHHHHHCCCCEEEEECCCCCEECHHHHHHHHHHCCCCCCCEEEHHH
VGNLVCPALFDLGEAAKVVVISVTEGDDKPLKYPTMFHEADICLLNKTDLLPYVDFDVAK
CCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEECCCCEEEEECCCCCCCCCCCHHH
CREYAMQVNHHLEWIEVSAKTGEGFDQWIAWLKTKLAAL
HHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCDTCGCSGDGGAVLRKPGVKDYHVHVGDDGHHHHHDHQHSHDHGHSHDHAHEHHHDHHH
CCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCC
GEARKVQMEQDVLLQNNMLAERNRGWFEARRVLALNFLSSPGSGKTSILEKTIPALLEQC
CCHHEEEHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
PVTVIEGDQQSTNDADRIDALGVPVIQVNTGTGCHLDAQMVQRAVRELDPPERSLVCIEN
CEEEEECCCCCCCCHHHHHHCCCCEEEEECCCCCEECHHHHHHHHHHCCCCCCCEEEHHH
VGNLVCPALFDLGEAAKVVVISVTEGDDKPLKYPTMFHEADICLLNKTDLLPYVDFDVAK
CCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEECCCCEEEEECCCCCCCCCCCHHH
CREYAMQVNHHLEWIEVSAKTGEGFDQWIAWLKTKLAAL
HHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]